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Cytokeratin 5 (KRT5) (NM_000424) Human Tagged ORF Clone
CAT＃: RC206870
KRT5 (Myc-DDK-tagged)-Human keratin 5 (KRT5)
ORF Plasmid: tGFP
"NM_000424" in other vectors (6)
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CNY 4,392.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CK5; DDD; DDD1; EBS2; K5; KRT5A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206870 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCGCCAGTCAAGTGTGTCCTTCCGGAGCGGGGGCAGTCGTAGCTTCAGCACCGCCTCTGCCATCA CCCCGTCTGTCTCCCGCACCAGCTTCACCTCCGTGTCCCGGTCCGGGGGTGGCGGTGGTGGTGGCTTCGG CAGGGTCAGCCTTGCGGGTGCTTGTGGAGTGGGTGGCTATGGCAGCCGGAGCCTCTACAACCTGGGGGGC TCCAAGAGGATATCCATCAGCACTAGTGGTGGCAGCTTCAGGAACCGGTTTGGTGCTGGTGCTGGAGGCG GCTATGGCTTTGGAGGTGGTGCCGGTAGTGGATTTGGTTTCGGCGGTGGAGCTGGTGGTGGCTTTGGGCT TGGTGGCGGAGCTGGCTTTGGAGGTGGCTTCGGTGGCCCTGGCTTTCCTGTCTGCCCTCCTGGAGGTATC CAAGAGGTCACTGTCAACCAGAGTCTCCTGACTCCCCTCAACCTGCAAATCGACCCCAGCATCCAGAGGG TGAGGACCGAGGAGCGCGAGCAGATCAAGACCCTCAACAATAAGTTTGCCTCCTTCATCGACAAGGTGCG GTTCCTGGAGCAGCAGAACAAGGTTCTGGACACCAAGTGGACCCTGCTGCAGGAGCAGGGCACCAAGACC GTGAGGCAGAACCTGGAGCCGTTGTTCGAGCAGTACATCAACAACCTCAGGAGGCAGCTGGACAGCATCG TGGGGGAACGGGGCCGCCTGGACTCAGAGCTAAGAAACATGCAGGACCTGGTGGAAGACTTCAAGAACAA GTATGAGGATGAAATCAACAAGCGTACCACTGCTGAGAATGAGTTTGTGATGCTGAAGAAGGATGTAGAT GCTGCCTACATGAACAAGGTGGAGCTGGAGGCCAAGGTTGATGCACTGATGGATGAGATTAACTTCATGA AGATGTTCTTTGATGCGGAGCTGTCCCAGATGCAGACGCATGTCTCTGACACCTCAGTGGTCCTCTCCAT GGACAACAACCGCAACCTGGACCTGGATAGCATCATCGCTGAGGTCAAGGCCCAGTATGAGGAGATTGCC AACCGCAGCCGGACCGAAGCCGAGTCCTGGTATCAGACCAAGTATGAGGAGCTGCAGCAGACAGCTGGCC GGCATGGCGATGACCTCCGCAACACCAAGCATGAGATCTCTGAGATGAACCGGATGATCCAGAGGCTGAG AGCCGAGATTGACAATGTCAAGAAACAGTGCGCCAATCTGCAGAACGCCATTGCGGATGCCGAGCAGCGT GGGGAGCTGGCCCTCAAGGATGCCAGGAACAAGCTGGCCGAGCTGGAGGAGGCCCTGCAGAAGGCCAAGC AGGACATGGCCCGGCTGCTGCGTGAGTACCAGGAGCTCATGAACACCAAGCTGGCCCTGGACGTGGAGAT CGCCACTTACCGCAAGCTGCTGGAGGGCGAGGAATGCAGACTCAGTGGAGAAGGAGTTGGACCAGTCAAC ATCTCTGTTGTCACAAGCAGTGTTTCCTCTGGATATGGCAGTGGCAGTGGCTATGGCGGTGGCCTCGGTG GAGGTCTTGGCGGCGGCCTCGGTGGAGGTCTTGCCGGAGGTGGCAGTGGAAGCTACTACTCCAGCAGCAG TGGGGGTGTCGGCCTAAGTGGTGGGCTCAGTGTGGGGGGCTCTGGCTTCAGTGCAAGCAGTGGCCGAGGG CTGGGGGTGGGCTTTGGCAGTGGCGGGGGTAGCAGCTCCAGCGTCAAATTTGTCTCCACCACCTCCTCCT CCCGGAAGAGCTTCAAGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206870 protein sequence
Red=Cloning site Green=Tags(s) MSRQSSVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGYGSRSLYNLGG SKRISISTSGGSFRNRFGAGAGGGYGFGGGAGSGFGFGGGAGGGFGLGGGAGFGGGFGGPGFPVCPPGGI QEVTVNQSLLTPLNLQIDPSIQRVRTEEREQIKTLNNKFASFIDKVRFLEQQNKVLDTKWTLLQEQGTKT VRQNLEPLFEQYINNLRRQLDSIVGERGRLDSELRNMQDLVEDFKNKYEDEINKRTTAENEFVMLKKDVD AAYMNKVELEAKVDALMDEINFMKMFFDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIA NRSRTEAESWYQTKYEELQQTAGRHGDDLRNTKHEISEMNRMIQRLRAEIDNVKKQCANLQNAIADAEQR GELALKDARNKLAELEEALQKAKQDMARLLREYQELMNTKLALDVEIATYRKLLEGEECRLSGEGVGPVN ISVVTSSVSSGYGSGSGYGGGLGGGLGGGLGGGLAGGGSGSYYSSSSGGVGLSGGLSVGGSGFSASSGRG LGVGFGSGGGSSSSVKFVSTTSSSRKSFKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000424 |
| ORF Size | 1770 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000424.4 |
| RefSeq Size | 2320 bp |
| RefSeq ORF | 1773 bp |
| Locus ID | 3852 |
| UniProt ID | P13647 |
| Domains | filament |
| MW | 62.4 kDa |
| Gene Summary | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206870L1 | Lenti ORF clone of Human keratin 5 (KRT5), Myc-DDK-tagged |
CNY 6,792.00 |
|
| RC206870L2 | Lenti ORF clone of Human keratin 5 (KRT5), mGFP tagged |
CNY 5,890.00 |
|
| RC206870L3 | Lenti ORF clone of Human keratin 5 (KRT5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206870L4 | Lenti ORF clone of Human keratin 5 (KRT5), mGFP tagged |
CNY 5,890.00 |
|
| RG206870 | KRT5 (tGFP-tagged) - Human keratin 5 (KRT5) |
CNY 5,992.00 |
|
| SC319550 | KRT5 (untagged)-Human keratin 5 (KRT5) |
CNY 4,400.00 |
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