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PPP2R5B (NM_006244) Human Tagged ORF Clone
CAT＃: RC206889
PPP2R5B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B', beta (PPP2R5B)
ORF Plasmid: tGFP
"NM_006244" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | B56B; B56beta; PR61B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206889 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACGAAGCTGCCCCCTGCAAGCACCCCCACTAGCCCCTCCTCCCCCGGGCTGTCGCCTGTGCCCC CACCCGACAAGGTGGACGGCTTCTCCCGCCGTTCCCTCCGCAGAGCCCGGCCCCGCCGCTCCCACAGCTC CTCTCAGTTCCGCTATCAGAGCAACCAGCAAGAGCTCACACCGCTGCCCCTGCTCAAAGATGTGCCGGCT TCCGAGCTGCACGAGCTGCTGAGCCGGAAGCTGGCCCAGTGTGGGGTGATGTTTGACTTCTTGGACTGTG TGGCCGACCTCAAGGGGAAGGAGGTGAAGCGGGCAGCCCTCAACGAGCTGGTGGAGTGTGTGGGGAGCAC CCGGGGTGTCCTCATCGAGCCCGTCTACCCAGACATCATCCGCATGATCTCAGTGAATATCTTCCGGACT CTGCCGCCCAGTGAGAACCCTGAATTTGACCCTGAAGAGGATGAGCCCAATCTTGAGCCTTCGTGGCCAC ACCTGCAGCTGGTATATGAGTTTTTCCTGCGTTTCTTGGAGAGCCCAGACTTCCAGCCCTCCGTGGCCAA GAGATATGTGGATCAAAAGTTTGTCCTGATGCTCCTGGAGCTATTTGATAGTGAGGATCCCCGGGAGCGT GAGTACCTCAAGACCATCCTGCACCGGGTCTATGGCAAGTTCCTGGGTCTCCGGGCCTACATCCGCAAAC AGTGCAACCACATCTTCCTCCGGTTCATCTATGAATTCGAGCACTTCAATGGTGTGGCTGAGCTGCTGGA GATCCTAGGAAGCATCATCAATGGCTTTGCGCTGCCCCTGAAGACGGAGCACAAGCAGTTCCTGGTTCGC GTCCTGATCCCCCTGCACTCTGTCAAGTCGCTGTCTGTCTTCCATGCCCAGCTGGCATACTGTGTGGTGC AGTTCCTGGAGAAGGATGCCACTCTGACAGAGCACGTGATCCGGGGGCTGCTCAAATACTGGCCAAAAAC CTGCACCCAGAAGGAGGTGATGTTTCTGGGGGAGATGGAAGAGATTCTTGATGTCATCGAGCCCTCCCAG TTTGTGAAGATCCAGGAGCCCCTTTTTAAGCAGGTGGCTCGCTGTGTTTCCAGCCCCCATTTCCAGGTTG CAGAGCGGGCTCTGTATTTCTGGAACAATGAGTATATCCTAAGCCTCATTGAGGACAACTGCCACACTGT GCTGCCTGCTGTGTTTGGGACCCTCTACCAAGTCTCCAAGGAGCACTGGAACCAAACCATCGTATCACTG ATCTACAATGTGCTCAAGACCTTCATGGAGATGAATGGGAAGCTGTTTGATGAGCTCACAGCCTCCTACA AGCTGGAAAAGCAGCAGGAGCAGCAGAAGGCCCAGGAGCGTCAGGAGTTATGGCAAGGTCTGGAGGAGCT GCGGCTACGCCGGCTACAGGGGACCCAGGGGGCCAAGGAGGCCCCCCTCCAGCGGCTTACACCCCAGGTG GCCGCCAGTGGGGGTCAGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206889 protein sequence
Red=Cloning site Green=Tags(s) METKLPPASTPTSPSSPGLSPVPPPDKVDGFSRRSLRRARPRRSHSSSQFRYQSNQQELTPLPLLKDVPA SELHELLSRKLAQCGVMFDFLDCVADLKGKEVKRAALNELVECVGSTRGVLIEPVYPDIIRMISVNIFRT LPPSENPEFDPEEDEPNLEPSWPHLQLVYEFFLRFLESPDFQPSVAKRYVDQKFVLMLLELFDSEDPRER EYLKTILHRVYGKFLGLRAYIRKQCNHIFLRFIYEFEHFNGVAELLEILGSIINGFALPLKTEHKQFLVR VLIPLHSVKSLSVFHAQLAYCVVQFLEKDATLTEHVIRGLLKYWPKTCTQKEVMFLGEMEEILDVIEPSQ FVKIQEPLFKQVARCVSSPHFQVAERALYFWNNEYILSLIEDNCHTVLPAVFGTLYQVSKEHWNQTIVSL IYNVLKTFMEMNGKLFDELTASYKLEKQQEQQKAQERQELWQGLEELRLRRLQGTQGAKEAPLQRLTPQV AASGGQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006244 |
| ORF Size | 1491 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006244.4 |
| RefSeq Size | 2766 bp |
| RefSeq ORF | 1494 bp |
| Locus ID | 5526 |
| UniProt ID | Q15173 |
| Domains | B56 |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Oocyte meiosis, Wnt signaling pathway |
| MW | 57.4 kDa |
| Gene Summary | The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B56 subfamily. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206889L1 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B', beta (PPP2R5B), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC206889L2 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B', beta (PPP2R5B), mGFP tagged |
CNY 5,890.00 |
|
| RC206889L3 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B', beta (PPP2R5B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206889L4 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B', beta (PPP2R5B), mGFP tagged |
CNY 5,890.00 |
|
| RG206889 | PPP2R5B (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B', beta (PPP2R5B) |
CNY 5,256.00 |
|
| SC110982 | PPP2R5B (untagged)-Human protein phosphatase 2, regulatory subunit B', beta (PPP2R5B) |
CNY 3,656.00 |
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