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STAT4 (NM_003151) Human Tagged ORF Clone
CAT＃: RC206892
STAT4 (Myc-DDK-tagged)-Human signal transducer and activator of transcription 4 (STAT4)
ORF Plasmid: tGFP
"NM_003151" in other vectors (6)
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CNY 5,480.00
CNY 5,800.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SLEB11 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206892 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCAGTGGAATCAAGTCCAACAGTTAGAAATCAAGTTTTTGGAGCAGGTGGATCAATTCTATGATG ACAACTTTCCCATGGAAATTCGGCATCTGTTGGCCCAATGGATTGAAAATCAAGACTGGGAGGCAGCTTC TAACAATGAAACCATGGCAACGATTCTTCTTCAAAACTTGTTAATACAACTGGATGAACAGTTAGGTCGT GTTTCCAAAGAGAAAAACCTACTCTTGATACACAATCTAAAAAGAATTAGGAAGGTCCTTCAGGGAAAAT TTCATGGAAATCCAATGCATGTAGCTGTGGTTATTTCAAACTGTTTAAGGGAAGAGAGGAGAATATTGGC TGCAGCCAACATGCCTGTCCAGGGGCCTCTAGAGAAATCCTTACAAAGTTCTTCAGTTTCAGAAAGACAG AGGAATGTGGAGCACAAAGTGGCTGCCATTAAAAACAGTGTGCAGATGACAGAACAAGATACCAAATACT TAGAAGATCTGCAAGACGAATTTGACTACAGGTATAAAACAATTCAGACAATGGATCAGAGTGACAAGAA TAGTGCCATGGTGAATCAGGAAGTTTTGACACTGCAGGAAATGCTTAACAGCCTCGATTTCAAGAGAAAG GAGGCTCTCAGTAAAATGACCCAAATCATCCATGAGACAGACCTGTTAATGAACACCATGCTCATAGAAG AGCTGCAAGACTGGAAGCGGCGGCAGCAAATCGCCTGCATCGGGGGTCCACTCCACAATGGGCTCGACCA GCTTCAGAACTGCTTTACACTATTGGCAGAAAGTCTTTTCCAACTGAGAAGGCAATTGGAGAAACTAGAG GAGCAATCTACCAAAATGACATATGAAGGTGATCCCATTCCAATGCAAAGAACTCACATGCTAGAAAGAG TCACCTTCTTGATCTACAACCTTTTCAAGAACTCATTTGTGGTTGAGCGACAGCCATGTATGCCAACCCA CCCTCAGAGGCCGTTGGTACTTAAAACCCTAATTCAGTTCACTGTAAAACTAAGGCTACTAATAAAATTG CCAGAACTAAACTATCAGGTAAAGGTTAAGGCATCAATTGACAAGAATGTTTCAACTCTAAGCAACCGAA GATTTGTACTTTGTGGAACTAATGTCAAAGCCATGTCTATTGAAGAATCTTCCAATGGGAGTCTCTCAGT AGAATTTCGACATTTGCAACCAAAGGAAATGAAGTCCAGTGCTGGAGGTAAAGGAAATGAGGGCTGTCAC ATGGTGACTGAAGAACTTCATTCCATAACGTTTGAAACACAGATCTGCCTCTATGGCCTGACCATAGATT TGGAGACCAGCTCATTGCCTGTGGTGATGATTTCCAATGTCAGTCAGTTACCTAATGCTTGGGCATCCAT CATTTGGTACAACGTGTCAACCAACGATTCCCAGAACTTGGTTTTCTTTAATAATCCTCCACCTGCCACA TTGAGTCAACTACTGGAGGTGATGAGCTGGCAGTTTTCATCGTACGTTGGTCGTGGTCTTAACTCAGATC AACTCCATATGCTGGCAGAGAAGCTTACAGTCCAATCTAGCTACAGTGATGGTCACCTCACCTGGGCCAA GTTCTGCAAGGAACATTTACCTGGTAAATCATTTACCTTTTGGACATGGCTTGAAGCAATATTGGATCTA ATTAAGAAACACATTCTTCCCCTTTGGATTGATGGGTATGTCATGGGCTTTGTTAGCAAAGAGAAGGAAC GGCTGTTGCTAAAGGATAAAATGCCTGGCACCTTTTTATTAAGATTCAGTGAAAGCCATCTCGGAGGAAT AACTTTCACCTGGGTGGACCATTCTGAAAGTGGGGAAGTGAGATTCCACTCTGTAGAACCCTACAATAAA GGCCGGTTGTCTGCTCTGCCATTCGCTGACATCCTGCGAGACTACAAAGTTATTATGGCTGAAAACATTC CTGAAAACCCTCTGAAGTACCTATATCCTGACATTCCCAAAGACAAAGCCTTCGGTAAACACTACAGCTC TCAGCCTTGCGAAGTTTCAAGACCAACAGAAAGGGGTGACAAAGGTTATGTTCCTTCTGTTTTTATCCCC ATCTCAACAATCCGAAGTGATTCAACAGAGCCACATTCTCCATCAGACCTTCTTCCCATGTCTCCAAGTG TGTATGCGGTGTTGAGAGAAAACCTGAGTCCCACAACAATTGAAACTGCAATGAAGTCTCCTTATTCTGC TGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206892 protein sequence
Red=Cloning site Green=Tags(s) MSQWNQVQQLEIKFLEQVDQFYDDNFPMEIRHLLAQWIENQDWEAASNNETMATILLQNLLIQLDEQLGR VSKEKNLLLIHNLKRIRKVLQGKFHGNPMHVAVVISNCLREERRILAAANMPVQGPLEKSLQSSSVSERQ RNVEHKVAAIKNSVQMTEQDTKYLEDLQDEFDYRYKTIQTMDQSDKNSAMVNQEVLTLQEMLNSLDFKRK EALSKMTQIIHETDLLMNTMLIEELQDWKRRQQIACIGGPLHNGLDQLQNCFTLLAESLFQLRRQLEKLE EQSTKMTYEGDPIPMQRTHMLERVTFLIYNLFKNSFVVERQPCMPTHPQRPLVLKTLIQFTVKLRLLIKL PELNYQVKVKASIDKNVSTLSNRRFVLCGTNVKAMSIEESSNGSLSVEFRHLQPKEMKSSAGGKGNEGCH MVTEELHSITFETQICLYGLTIDLETSSLPVVMISNVSQLPNAWASIIWYNVSTNDSQNLVFFNNPPPAT LSQLLEVMSWQFSSYVGRGLNSDQLHMLAEKLTVQSSYSDGHLTWAKFCKEHLPGKSFTFWTWLEAILDL IKKHILPLWIDGYVMGFVSKEKERLLLKDKMPGTFLLRFSESHLGGITFTWVDHSESGEVRFHSVEPYNK GRLSALPFADILRDYKVIMAENIPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIP ISTIRSDSTEPHSPSDLLPMSPSVYAVLRENLSPTTIETAMKSPYSAE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003151 |
| ORF Size | 2244 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003151.4 |
| RefSeq Size | 2840 bp |
| RefSeq ORF | 2247 bp |
| Locus ID | 6775 |
| UniProt ID | Q14765 |
| Domains | SH2, STAT |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Jak-STAT signaling pathway |
| MW | 85.9 kDa |
| Gene Summary | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Paracoccidioidomycosis associated with a heterozygous STAT4 mutation and impaired IFN-γ immunity
,Schimke, LF;Hibbard, J;Martinez-Barricarte, R;Khan, TA;de Souza Cavalcante, R;Borges de Oliveira Junior, E;Takahashi França, T;Iqbal, A;Yamamoto, G;Arslanian, C;Feriotti, C;da Costa, TA;Bustamante, J;Boisson-Dupuis, S;Casanova, JL;Marzagao Barbuto, JA;Zatz, M;Poncio Mendes, R;Garcia Calich, VL;Ochs, HD;Torgerson, TR;Cabral-Marques, O;Condino-Neto, A;,
J. Infect. Dis.
,PubMed ID 29029192
[STAT4]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206892L1 | Lenti ORF clone of Human signal transducer and activator of transcription 4 (STAT4), Myc-DDK-tagged |
CNY 7,880.00 |
|
| RC206892L2 | Lenti ORF clone of Human signal transducer and activator of transcription 4 (STAT4), mGFP tagged |
CNY 7,700.00 |
|
| RC206892L3 | Lenti ORF clone of Human signal transducer and activator of transcription 4 (STAT4), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC206892L4 | Lenti ORF clone of Human signal transducer and activator of transcription 4 (STAT4), mGFP tagged |
CNY 7,700.00 |
|
| RG206892 | STAT4 (tGFP-tagged) - Human signal transducer and activator of transcription 4 (STAT4) |
CNY 7,080.00 |
|
| SC118193 | STAT4 (untagged)-Human signal transducer and activator of transcription 4 (STAT4) |
CNY 5,488.00 |
