PALM2AKAP2 (NM_001037293) Human Tagged ORF Clone

CAT#: RC206919

PALM2 (Myc-DDK-tagged)-Human paralemmin 2 (PALM2), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001037293" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal PALM2 Antibody
    • 100 ug

CNY 5,302.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms AKAP-2; AKAP-KL; AKAP2; AKAPKL; MISP2; PALM2; PALM2-AKAP2; PRKA2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206919 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGGCGGAATTGCACAAGGAAAGGCTGCAAGCCATAGCAGAAAAAAGAAAGAGGCAGACTGAAA
TAGAAGGCAAGCGACAACAGCTTGACGAGCAGATACTTCTGCTGCAGCATTCCAAGTCCAAAGTGCTTCG
GGAGAAATGGCTGCTGCAGGGCATACCCGCTGGAACTGCCGAAGAGGAGGAAGCCAGGAGGCGGCAGTCT
GAAGAGGATGAGTTCAGAGTCAAGCAACTTGAAGATAACATTCAGAGGCTGGAGCAAGAAATACAAACGC
TAGAAAGTGAAGAGTCCCGGATATCTGCCAAAGAGCAAATCATCCTAGAGAAACTGAAGGAAACAGAAAA
ATCCTTCAAGGACTTTCAGAAGGGTTTCTCCAGTACGGATGGAGCTGTGTACGCCATGGAAATTAATGTG
GAGAAAGACAAACAAACAGGAGAGACCAAGATCCTCTCTACATCTACCATTGGCCCAGAGGGGGTCCATC
AGAAAGGAGTCAAAGTCTATGATGATGGTACCAAAGTAGTGTATGAGGTGCGCTCAGGAGGCACCGTAGT
AGAAAATGGAGTGCACAAATTAAGCACAAAGGATGTAGAAGAGCTTATTCAGAAGGCTGGACAATCAAGC
TTAGGAGGAGGGCACGTGTCTGAAAGGACTGTGATTGCAGATGGGAGCCTCAGCCATCCCAAGGAACACA
TGCTCTGCAAAGAAGCTAAGTTAGAAATGGTACATAAGTCTAGGAAAGACCATTCTTCCGGGAACCCAGG
GCAGCAGGCCCAAGCCCCCAGCGCTGCAGGGCCGGAGGCAAACTTGGATCAGCCCGTCACCATGATTTTT
ATGGGCTACCAAAATATCGAGGATGAAGAGGAGACGAAAAAGGTGCTAGGCTATGATGAAACCATCAAGG
CTGAATTGGTCCTCATTGATGAAGATGATGAGAAGTCATTGAGGGAGAAGACAGTGACGGACGTGTCCAC
TATTGACGGGAACGCGGCTGAGCTTGTGTCCGGGAGGCCGGTCTCAGACACCACAGAGCCCTCATCCCCA
GAAGGGAAGGAAGAGAGCCTAGCTACAGAGCCAGCCCCAGGTACCCAAAAGAAAAAGCGCTGTCAATGCT
GTGTTGTCATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206919 protein sequence
Red=Cloning site Green=Tags(s)

MAEAELHKERLQAIAEKRKRQTEIEGKRQQLDEQILLLQHSKSKVLREKWLLQGIPAGTAEEEEARRRQS
EEDEFRVKQLEDNIQRLEQEIQTLESEESRISAKEQIILEKLKETEKSFKDFQKGFSSTDGAVYAMEINV
EKDKQTGETKILSTSTIGPEGVHQKGVKVYDDGTKVVYEVRSGGTVVENGVHKLSTKDVEELIQKAGQSS
LGGGHVSERTVIADGSLSHPKEHMLCKEAKLEMVHKSRKDHSSGNPGQQAQAPSAAGPEANLDQPVTMIF
MGYQNIEDEEETKKVLGYDETIKAELVLIDEDDEKSLREKTVTDVSTIDGNAAELVSGRPVSDTTEPSSP
EGKEESLATEPAPGTQKKKRCQCCVVM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001037293
ORF Size 1131 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001037293.2, NP_001032370.1
RefSeq Size 9286 bp
RefSeq ORF 1140 bp
Locus ID 445815
UniProt ID Q8IXS6
MW 42 kDa
Gene Summary This gene belongs to the paralemmin downstream gene (PDG) family defined in PMID:22855693. Paralemmin downstream genes may have evolved contiguously with the paralemmin genes and are associated with other paralemmin paralogs in humans and several other taxa. The gene encodes three distinct protein isoforms, the PALM2 isoform, the AKAP2 isoform and the PALM2-AKAP2 isoform. The biological significance of the PALM2-AKAP2 isoforms is yet unknown. Earlier, PALM2 and AKAP2 were annotated as separate genes and PALM2-AKAP2 was annotated as a readthrough gene. [provided by RefSeq, May 2019]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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