Rel B (RELB) (NM_006509) Human Tagged ORF Clone
CAT#: RC207006
- TrueORF®
RELB (Myc-DDK-tagged)-Human v-rel reticuloendotheliosis viral oncogene homolog B (RELB)
ORF Plasmid: tGFP
"NM_006509" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | I-REL; IMD53; IREL; REL-B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207006 representing NM_006509
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCGGTCTGGGCCAGCCTCTGGGCCGTCCGTCCCCACTGGCCGGGCCATGCCGAGTCGCCGCGTCG CCAGACCGCCGGCTGCGCCGGAGCTGGGGGCCTTAGGGTCCCCCGACCTCTCCTCACTCTCGCTCGCCGT TTCCAGGAGCACAGATGAATTGGAGATCATCGACGAGTACATCAAGGAGAACGGCTTCGGCCTGGACGGG GGACAGCCGGGCCCGGGCGAGGGGCTGCCACGCCTGGTGTCTCGCGGGGCTGCGTCCCTGAGCACGGTCA CCCTGGGCCCTGTGGCGCCCCCAGCCACGCCGCCGCCTTGGGGCTGCCCCCTGGGCCGACTAGTGTCCCC AGCGCCGGGCCCGGGCCCGCAGCCGCACCTGGTCATCACGGAGCAGCCCAAGCAGCGCGGCATGCGCTTC CGCTACGAGTGCGAGGGCCGCTCGGCCGGCAGCATCCTTGGGGAGAGCAGCACCGAGGCCAGCAAGACGC TGCCCGCCATCGAGCTCCGGGATTGTGGAGGGCTGCGGGAGGTGGAGGTGACTGCCTGCCTGGTGTGGAA GGACTGGCCTCACCGAGTCCACCCCCACAGCCTCGTGGGGAAAGACTGCACCGACGGCATCTGCAGGGTG CGGCTCCGGCCTCACGTCAGCCCCCGGCACAGTTTTAACAACCTGGGCATCCAGTGTGTGAGGAAGAAGG AGATTGAGGCTGCCATTGAGCGGAAGATTCAACTGGGCATTGACCCCTACAACGCTGGGTCCCTGAAGAA CCATCAGGAAGTAGACATGAATGTGGTGAGGATCTGCTTCCAGGCCTCATATCGGGACCAGCAGGGACAG ATGCGCCGGATGGATCCTGTGCTTTCCGAGCCCGTCTATGACAAGAAATCCACAAACACATCAGAGCTGC GGATTTGCCGAATTAACAAGGAAAGCGGGCCGTGCACCGGTGGCGAGGAGCTCTACTTGCTCTGCGACAA GGTGCAGAAAGAGGACATATCAGTGGTGTTCAGCAGGGCCTCCTGGGAAGGTCGGGCTGACTTCTCCCAG GCCGACGTGCACCGCCAGATTGCCATTGTGTTCAAGACGCCGCCCTACGAGGACCTGGAGATTGTCGAGC CCGTGACAGTCAACGTCTTCCTGCAGCGGCTCACCGATGGGGTCTGCAGCGAGCCATTGCCTTTCACGTA CCTGCCTCGCGACCATGACAGCTACGGCGTGGACAAGAAGCGGAAACGGGGGATGCCCGACGTCCTTGGG GAGCTGAACAGCTCTGACCCCCATGGCATCGAGAGCAAACGGCGGAAGAAAAAGCCGGCCATCCTGGACC ACTTCCTGCCCAACCACGGCTCAGGCCCGTTCCTCCCGCCGTCAGCCCTGCTGCCAGACCCTGACTTCTT CTCTGGCACCGTGTCCCTGCCCGGCCTGGAGCCCCCTGGCGGGCCTGACCTCCTGGACGATGGCTTTGCC TACGACCCTACGGCCCCCACACTCTTCACCATGCTGGACCTGCTGCCCCCGGCACCGCCACACGCTAGCG CTGTTGTGTGCAGCGGAGGTGCCGGGGCCGTGGTTGGGGAGACCCCCGGCCCTGAACCACTGACACTGGA CTCGTACCAGGCCCCGGGCCCCGGGGATGGAGGCACCGCCAGCCTTGTGGGCAGCAACATGTTCCCCAAT CATTACCGCGAGGCGGCCTTTGGGGGCGGCCTCCTATCCCCGGGGCCTGAAGCCACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207006 representing NM_006509
Red=Cloning site Green=Tags(s) MLRSGPASGPSVPTGRAMPSRRVARPPAAPELGALGSPDLSSLSLAVSRSTDELEIIDEYIKENGFGLDG GQPGPGEGLPRLVSRGAASLSTVTLGPVAPPATPPPWGCPLGRLVSPAPGPGPQPHLVITEQPKQRGMRF RYECEGRSAGSILGESSTEASKTLPAIELRDCGGLREVEVTACLVWKDWPHRVHPHSLVGKDCTDGICRV RLRPHVSPRHSFNNLGIQCVRKKEIEAAIERKIQLGIDPYNAGSLKNHQEVDMNVVRICFQASYRDQQGQ MRRMDPVLSEPVYDKKSTNTSELRICRINKESGPCTGGEELYLLCDKVQKEDISVVFSRASWEGRADFSQ ADVHRQIAIVFKTPPYEDLEIVEPVTVNVFLQRLTDGVCSEPLPFTYLPRDHDSYGVDKKRKRGMPDVLG ELNSSDPHGIESKRRKKKPAILDHFLPNHGSGPFLPPSALLPDPDFFSGTVSLPGLEPPGGPDLLDDGFA YDPTAPTLFTMLDLLPPAPPHASAVVCSGGAGAVVGETPGPEPLTLDSYQAPGPGDGGTASLVGSNMFPN HYREAAFGGGLLSPGPEAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_006509 |
ORF Size | 1737 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006509.4 |
RefSeq Size | 2287 bp |
RefSeq ORF | 1740 bp |
Locus ID | 5971 |
UniProt ID | Q01201 |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | MAPK signaling pathway |
MW | 62 kDa |
Gene Summary | NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer in a CRY1/CRY2 independent manner. Increased repression of the heterodimer is seen in the presence of NFKB2/p52. Is required for both T and B lymphocyte maturation and function (PubMed:26385063).[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
The RelB alternative NF-kappaB subunit promotes autophagy in 22Rv1 prostate cancer cells in vitro and affects mouse xenograft tumor growth in vivo
,Labouba, I;Poisson, A;Lafontaine, J;Delvoye, N;,
cancer cell microenvironment
[Rel B]
|
Glycogen synthase kinase-3ß is a crucial mediator of signal-induced RelB degradation
,M Neumann, S Klar, A Wilisch-Neumann, E Hollenbach, S Kavuri, M Leverkus, R Kandolf, M C Brunner-Weinzierl, K Klingel,
Oncogene (10 January 2011) doi:10.1038/onc.2010.580 Short Communication
[Rel B]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207006L1 | Lenti ORF clone of Human v-rel reticuloendotheliosis viral oncogene homolog B (RELB), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC207006L2 | Lenti ORF clone of Human v-rel reticuloendotheliosis viral oncogene homolog B (RELB), mGFP tagged |
CNY 5,890.00 |
|
RC207006L3 | Lenti ORF clone of Human v-rel reticuloendotheliosis viral oncogene homolog B (RELB), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC207006L4 | Lenti ORF clone of Human v-rel reticuloendotheliosis viral oncogene homolog B (RELB), mGFP tagged |
CNY 5,890.00 |
|
RG207006 | RELB (tGFP-tagged) - Human v-rel reticuloendotheliosis viral oncogene homolog B (RELB) |
CNY 4,370.00 |
|
SC122747 | RELB (untagged)-Human v-rel reticuloendotheliosis viral oncogene homolog B (RELB) |
CNY 2,950.00 |