Neurogenin 1 (NEUROG1) (NM_006161) Human Tagged ORF Clone

CAT#: RC207029

NEUROG1 (Myc-DDK-tagged)-Human neurogenin 1 (NEUROG1)

ORF Plasmid: DDK tGFP



  "NM_006161" in other vectors (6)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


NEUROG1 (Neurogenin 1) mouse monoclonal antibody, clone OTI3F9 (formerly 3F9)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms AKA; bHLHa6; Math4C; NEUROD3; ngn1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207029 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGCCCGCCTTGAGACCTGCATCTCCGACCTCGACTGCGCCAGCAGCAGCGGCAGTGACCTATCCG
GCTTCCTCACCGACGAGGAAGACTGTGCCAGACTCCAACAGGCAGCCTCCGCTTCGGGGCCGCCCGCGCC
GGCCCGCAGGGGCGCGCCCAATATCTCCCGGGCGTCTGAGGTTCCAGGGGCACAGGACGACGAGCAGGAG
AGGCGGCGGCGCCGCGGCCGGACGCGGGTCCGCTCCGAGGCGCTGCTGCACTCGCTGCGCAGGAGCCGGC
GCGTCAAGGCCAACGATCGCGAGCGCAACCGCATGCACAACTTGAACGCGGCCCTGGACGCACTGCGCAG
CGTGCTGCCCTCGTTCCCCGACGACACCAAGCTCACCAAAATCGAGACGCTGCGCTTCGCCTACAACTAC
ATCTGGGCTCTGGCCGAGACACTGCGCCTGGCGGATCAAGGGCTGCCCGGAGGCGGTGCCCGGGAGCGCC
TCCTGCCGCCGCAGTGCGTCCCCTGCCTGCCCGGTCCCCCAAGCCCCGCCAGCGACGCGGAGTCCTGGGG
CTCAGGTGCCGCCGCCGCCTCCCCGCTCTCTGACCCCAGTAGCCCAGCCGCCTCCGAAGACTTCACCTAC
CGCCCCGGCGACCCTGTTTTCTCCTTCCCAAGCCTGCCCAAAGACTTGCTCCACACAACGCCCTGTTTCA
TTCCTTACCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207029 protein sequence
Red=Cloning site Green=Tags(s)

MPARLETCISDLDCASSSGSDLSGFLTDEEDCARLQQAASASGPPAPARRGAPNISRASEVPGAQDDEQE
RRRRRGRTRVRSEALLHSLRRSRRVKANDRERNRMHNLNAALDALRSVLPSFPDDTKLTKIETLRFAYNY
IWALAETLRLADQGLPGGGARERLLPPQCVPCLPGPPSPASDAESWGSGAAAASPLSDPSSPAASEDFTY
RPGDPVFSFPSLPKDLLHTTPCFIPYH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006161
ORF Size 711 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006161.3
RefSeq Size 1717 bp
RefSeq ORF 714 bp
Locus ID 4762
UniProt ID Q92886
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
MW 25.7 kDa
Gene Summary Acts as a transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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