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LMAN1 (NM_005570) Human Tagged ORF Clone
CAT＃: RC207088
LMAN1 (Myc-DDK-tagged)-Human lectin, mannose-binding, 1 (LMAN1)
ORF Plasmid: tGFP
"NM_005570" in other vectors (6)
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CNY 3,800.00
CNY 3,990.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ERGIC-53; ERGIC53; F5F8D; FMFD1; gp58; MCFD1; MR60 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207088 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGATCCAGGCAAAGGGGTCTCCGGGCCAGAGTTCGGCCGCTGTTCTGCGCCTTGCTGCTGTCAC TCGGTCGCTTCGTCCGGGGCGACGGCGTGGGAGGAGACCCCGCGGTCGCGTTGCCACATCGCCGTTTCGA GTACAAATACAGCTTCAAGGGGCCGCACCTGGTGCAGAGCGACGGGACCGTGCCCTTCTGGGCCCACGCG GGGAATGCTATTCCAAGTTCAGATCAAATTCGAGTAGCACCATCTTTAAAAAGCCAAAGAGGCTCAGTGT GGACAAAGACAAAAGCGGCCTTTGAGAACTGGGAAGTTGAGGTGACATTTCGAGTGACTGGAAGAGGTCG AATTGGAGCTGATGGCCTAGCAATTTGGTATGCAGAAAATCAAGGCTTGGAGGGCCCTGTGTTTGGATCA GCTGATCTGTGGAATGGTGTTGGAATATTTTTTGATTCTTTTGACAATGATGGAAAGAAAAATAATCCTG CTATAGTAATTATAGGCAACAATGGACAAATCCATTATGACCATCAAAATGACGGGGCTAGTCAAGCTTT GGCAAGTTGCCAGAGGGACTTCCGCAACAAACCCTATCCTGTCCGAGCAAAGATTACCTATTACCAGAAC ACACTGACAGTAATGATCAATAATGGCTTTACACCAGATAAAAATGATTATGAATTTTGTGCCAAAGTGG AAAATATGATTATCCCTGCACAAGGGCATTTTGGAATATCTGCTGCAACTGGAGGTCTTGCAGATGACCA TGATGTCCTTTCTTTTCTGACTTTCCAGTTGACTGAACCTGGAAAAGAGCCGCCCACACCAGATAAAGAA ATTTCGGAAAAGGAAAAAGAAAAGTATCAGGAGGAATTTGAGCACTTTCAACAAGAATTGGATAAAAAAA AAGAGGAATTCCAGAAGGGCCACCCCGACCTCCAAGGGCAGCCTGCGGAGGAAATATTTGAGAGTGTAGG AGATCGAGAGCTAAGACAAGTCTTTGAAGGACAGAATCGTATTCATCTTGAAATCAAGCAGCTGAACCGG CAGTTAGATATGATTCTTGATGAACAGAGAAGATATGTCTCTTCCTTAACAGAGGAAATCTCTAAAAGAG GAGCAGGAATGCCTGGGCAGCATGGGCAGATTACTCAACAAGAACTGGATACTGTTGTGAAAACTCAGCA TGAGATTCTGAGACAAGTAAATGAAATGAAAAATTCCTTGAGTGAAACCGTCAGACTGGTCAGTGGAATG CAGCACCCTGGCTCTGCTGGAGGCGTCTATGAGACAACACAGCACTTCATTGACATCAAAGAGCACCTGC ACATAGTAAAGAGGGACATAGATAACTTAGTGCAGCGAAATATGCCATCAAATGAAAAGCCGAAATGCCC AGAACTACCACCATTTCCATCATGTTTGTCTACGGTCCACTTCATTATATTTGTTGTGGTGCAAACTGTA TTATTCATTGGTTATATCATGTATAGGTCTCAGCAAGAAGCAGCTGCCAAAAAATTCTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207088 protein sequence
Red=Cloning site Green=Tags(s) MAGSRQRGLRARVRPLFCALLLSLGRFVRGDGVGGDPAVALPHRRFEYKYSFKGPHLVQSDGTVPFWAHA GNAIPSSDQIRVAPSLKSQRGSVWTKTKAAFENWEVEVTFRVTGRGRIGADGLAIWYAENQGLEGPVFGS ADLWNGVGIFFDSFDNDGKKNNPAIVIIGNNGQIHYDHQNDGASQALASCQRDFRNKPYPVRAKITYYQN TLTVMINNGFTPDKNDYEFCAKVENMIIPAQGHFGISAATGGLADDHDVLSFLTFQLTEPGKEPPTPDKE ISEKEKEKYQEEFEHFQQELDKKKEEFQKGHPDLQGQPAEEIFESVGDRELRQVFEGQNRIHLEIKQLNR QLDMILDEQRRYVSSLTEEISKRGAGMPGQHGQITQQELDTVVKTQHEILRQVNEMKNSLSETVRLVSGM QHPGSAGGVYETTQHFIDIKEHLHIVKRDIDNLVQRNMPSNEKPKCPELPPFPSCLSTVHFIIFVVVQTV LFIGYIMYRSQQEAAAKKFF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005570 |
| ORF Size | 1530 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005570.4 |
| RefSeq Size | 4848 bp |
| RefSeq ORF | 1533 bp |
| Locus ID | 3998 |
| UniProt ID | P49257 |
| Domains | Lectin_leg-like |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 57.5 kDa |
| Gene Summary | The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency. [provided by RefSeq, Jul 2015] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A reference library for assigning protein subcellular localizations by image-based machine learning
,Schormann, W;Hariharan, S;Andrews, DW;,
J. Cell Biol.
,PubMed ID 31968357
[LMAN1]
|
|
Cholix protein domain I functions as a carrier element for efficient apical to basal epithelial transcytosis
,Taverner, A;MacKay, J;Laurent, F;Hunter, T;Liu, K;Mangat, K;Song, L;Seto, E;Postlethwaite, S;Alam, A;Chandalia, A;Seung, M;Saberi, M;Feng, W;Mrsny, RJ;,
Tissue Barriers
,PubMed ID 31928299
[LMAN1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207088L1 | Lenti ORF clone of Human lectin, mannose-binding, 1 (LMAN1), Myc-DDK-tagged |
CNY 6,200.00 |
|
| RC207088L2 | Lenti ORF clone of Human lectin, mannose-binding, 1 (LMAN1), mGFP tagged |
CNY 5,890.00 |
|
| RC207088L3 | Lenti ORF clone of Human lectin, mannose-binding, 1 (LMAN1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207088L4 | Lenti ORF clone of Human lectin, mannose-binding, 1 (LMAN1), mGFP tagged |
CNY 5,890.00 |
|
| RG207088 | LMAN1 (tGFP-tagged) - Human lectin, mannose-binding, 1 (LMAN1) |
CNY 5,400.00 |
|
| SC125316 | LMAN1 (untagged)-Human lectin, mannose-binding, 1 (LMAN1) |
CNY 3,808.00 |
