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AKAP3 (NM_006422) Human Tagged ORF Clone
CAT＃: RC207199
- TrueORF®
AKAP3 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 3 (AKAP3)
ORF Plasmid: tGFP
"NM_006422" in other vectors (4)
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CNY 6,248.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AKAP 110; AKAP110; CT82; FSP95; HEL159; PRKA3; SOB1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207199 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGAAAAGGTTGACTGGTTACAAAGCCAAAATGGAGTATGCAAAGTTGATGTCTATTCTCCTGGAG ACAACCAAGCCCAGGACTGGAAAATGGACACCTCCACGGATCCTGTCAGAGTGCTCAGCTGGCTCCGCAG AGACCTGGAGAAGAGTACAGCAGAGTTCCAAGATGTTCGGTTCAAACCCGGAGAATCATTTGGTGGGGAA ACGTCCAACTCAGGAGACCCACACAAAGGTTTCTCTGTAGACTATTACAACACCACCACCAAGGGCACTC CAGAAAGATTGCATTTTGAGATGACTCACAAAGAGATTCCTTGCCAGGGCCCCAGGGCCCAACTTGGCAA CGAGAGTTCAGTAGATGAAGTTTCCTTCTATGCTAACCGCCTCACGAATCTAGTCATAGCCATGGCCCGC AAAGAGATCAATGAGAAGATCGATGGCTCTGAAAACAAATGTGTCTATCAGTCATTGTACATGGGGAATG AACCCACACCCACCAAAAGCCTCAGTAAGATAGCATCAGAGCTTGTGAATGAGACCGTCTCTGCATGTTC CAGGAATGCTGCCCCAGACAAGGCTCCTGGCTCTGGAGACAGAGTCTCAGGATCATCACAAAGTCCCCCA AATTTGAAATACAAGTCCACTTTGAAGATCAAGGAGAGCACCAAAGAAAGACAGGGTCCAGATGACAAGC CTCCTTCTAAGAAGTCTTTCTTCTATAAGGAAGTGTTTGAATCTCGTAACGGAGATTATGCCAGAGAGGG TGGAAGGTTCTTTCCTCGGGAGAGAAAGAGGTTTCGAGGGCAGGAAAGGCCTGATGACTTTACGGCTTCT GTTAGTGAAGGGATCATGACCTATGCTAACAGTGTGGTATCTGATATGATGGTCTCCATCATGAAGACAC TGAAGATCCAAGTGAAAGACACAACCATTGCCACCATCCTACTGAAGAAGGTTCTGCTCAAGCATGCAAA AGAGGTGGTCTCGGATCTCATCGACTCCTTCTTGAGGAATCTCCACAGCGTCACAGGGACCCTCATGACT GACACACAGTTTGTCTCGGCTGTGAAAAGAACTGTCTTCTCTCATGGAAGCCAAAAGGCCACAGATATCA TGGATGCCATGCTAAGGAAGCTGTACAATGTAATGTTTGCCAAGAAAGTCCCTGAGCATGTCAGGAAAGC CCAAGACAAGGCTGAGAGTTATTCCCTCATCTCCATGAAAGGAATGGGTGATCCTAAAAACCGAAATGTG AACTTTGCCATGAAATCTGAAACTAAATTGAGAGAAAAAATGTATTCTGAACCCAAATCAGAGGAGGAGA CTTGTGCGAAAACTCTGGGTGAGCACATTATCAAAGAGGGGCTTACCCTGTGGCATAAAAGTCAGCAGAA AGAATGTAAATCTCTAGGTTTCCAGCATGCAGCATTCGAAGCTCCCAACACACAGCGTAAGCCTGCATCA GACATTTCCTTTGAGTACCCTGAAGATATTGGCAACCTCAGCCTTCCTCCATATCCTCCAGAGAAACCTG AGAATTTTATGTATGATTCAGACTCCTGGGCCAAGGACCTGATCGTGTCTGCCCTGCTTCTGATTCAATA TCACCTGGCCCAGGGAGGAAGAAGGGATGCACGGAGCTTCGTTGAAGCTGCTGGCACCACCAACTTTCCT GCCAATGAACCTCCTGTAGCTCCCGATGAATCTTGCCTTAAGTCTGCTCCCATTGTAGGTGACCAAGAAC AAGCAGAAAAGAAGGACCTAAGGAGTGTTTTCTTTAATTTCATCCGGAACTTACTTAGTGAGACCATTTT CAAGCGTGACCAGAGCCCTGAACCCAAGGTGCCGGAACAGCCAGTTAAGGAAGATAGGAAGTTGTGTGAA AGACCGTTGGCGTCTTCTCCCCCCAGGCTATATGAGGATGATGAGACCCCTGGTGCCCTTTCTGGGCTGA CCAAGATGGCTGTCAGCCAGATAGATGGCCACATGAGTGGGCAGATGGTAGAACATCTGATGAACTCAGT GATGAAGCTGTGTGTCATCATTGCTAAGTCCTGTGATGCTTCGTTGGCAGAGCTGGGAGATGACAAGTCT GGAGATGCCAGTAGGCTAACTTCGGCCTTCCCAGATAGTTTATATGAGTGCTTACCAGCCAAGGGCACAG GGTCAGCAGAAGCTGTCCTGCAGAATGCCTATCAAGCTATCCATAATGAAATGAGAGGCACATCAGGACA GCCCCCTGAAGGGTGTGCAGCACCCACGGTGATTGTCAGCAATCACAACCTAACGGACACAGTTCAGAAC AAGCAACTCCAAGCCGTCCTCCAATGGGTAGCTGCCTCTGAGCTCAATGTCCCTATTTTGTATTTTGCTG GTGATGATGAAGGGATCCAGGAGAAGCTACTTCAGCTCTCAGCTGCTGCTGTGGACAAAGGATGCAGTGT GGGCGAGGTTCTGCAGTCGGTGCTGCGCTATGAGAAGGAGCGCCAGCTGAATGAGGCGGTGGGGAATGTC ACACCGCTGCAGCTGCTGGACTGGCTGATGGTGAACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207199 protein sequence
Red=Cloning site Green=Tags(s) MSEKVDWLQSQNGVCKVDVYSPGDNQAQDWKMDTSTDPVRVLSWLRRDLEKSTAEFQDVRFKPGESFGGE TSNSGDPHKGFSVDYYNTTTKGTPERLHFEMTHKEIPCQGPRAQLGNESSVDEVSFYANRLTNLVIAMAR KEINEKIDGSENKCVYQSLYMGNEPTPTKSLSKIASELVNETVSACSRNAAPDKAPGSGDRVSGSSQSPP NLKYKSTLKIKESTKERQGPDDKPPSKKSFFYKEVFESRNGDYAREGGRFFPRERKRFRGQERPDDFTAS VSEGIMTYANSVVSDMMVSIMKTLKIQVKDTTIATILLKKVLLKHAKEVVSDLIDSFLRNLHSVTGTLMT DTQFVSAVKRTVFSHGSQKATDIMDAMLRKLYNVMFAKKVPEHVRKAQDKAESYSLISMKGMGDPKNRNV NFAMKSETKLREKMYSEPKSEEETCAKTLGEHIIKEGLTLWHKSQQKECKSLGFQHAAFEAPNTQRKPAS DISFEYPEDIGNLSLPPYPPEKPENFMYDSDSWAKDLIVSALLLIQYHLAQGGRRDARSFVEAAGTTNFP ANEPPVAPDESCLKSAPIVGDQEQAEKKDLRSVFFNFIRNLLSETIFKRDQSPEPKVPEQPVKEDRKLCE RPLASSPPRLYEDDETPGALSGLTKMAVSQIDGHMSGQMVEHLMNSVMKLCVIIAKSCDASLAELGDDKS GDASRLTSAFPDSLYECLPAKGTGSAEAVLQNAYQAIHNEMRGTSGQPPEGCAAPTVIVSNHNLTDTVQN KQLQAVLQWVAASELNVPILYFAGDDEGIQEKLLQLSAAAVDKGCSVGEVLQSVLRYEKERQLNEAVGNV TPLQLLDWLMVNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006422 |
| ORF Size | 2559 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006422.3 |
| RefSeq Size | 3323 bp |
| RefSeq ORF | 2562 bp |
| Locus ID | 10566 |
| UniProt ID | O75969 |
| Protein Families | Druggable Genome |
| MW | 94.8 kDa |
| Gene Summary | This gene encodes a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. The encoded protein is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. This protein is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction. [provided by RefSeq, May 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207199L3 | Lenti ORF clone of Human A kinase (PRKA) anchor protein 3 (AKAP3), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC207199L4 | Lenti ORF clone of Human A kinase (PRKA) anchor protein 3 (AKAP3), mGFP tagged |
CNY 9,030.00 |
|
| RG207199 | AKAP3 (tGFP-tagged) - Human A kinase (PRKA) anchor protein 3 (AKAP3) |
CNY 7,848.00 |
|
| SC126490 | AKAP3 (untagged)-Human A kinase (PRKA) anchor protein 3 (AKAP3) |
CNY 6,256.00 |
