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MMP15 (NM_002428) Human Tagged ORF Clone
CAT＃: RC207318
MMP15 (Myc-DDK-tagged)-Human matrix metallopeptidase 15 (membrane-inserted) (MMP15)
ORF Plasmid: tGFP
"NM_002428" in other vectors (6)
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CNY 4,904.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MMP-15; MT2-MMP; MT2MMP; MTMMP2; SMCP-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207318 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGCGACCCGAGCGCGCCCGGACGGCCGGGCTGGACGGGCAGCCTCCTCGGCGACCGGGAGGAGG CGGCGCGGCCGCGACTGCTGCCGCTGCTCCTGGTGCTTCTGGGCTGCCTGGGCCTTGGCGTAGCGGCCGA AGACGCGGAGGTCCATGCCGAGAACTGGCTGCGGCTTTATGGCTACCTGCCTCAGCCCAGCCGCCATATG TCCACCATGCGTTCCGCCCAGATCTTGGCCTCGGCCCTTGCAGAGATGCAGCGCTTCTACGGGATCCCAG TCACCGGTGTGCTCGACGAAGAGACCAAGGAGTGGATGAAGCGGCCCCGCTGTGGGGTGCCAGACCAGTT CGGGGTACGAGTGAAAGCCAACCTGCGGCGGCGTCGGAAGCGCTACGCCCTCACCGGGAGGAAGTGGAAC AACCACCATCTGACCTTTAGCATCCAGAACTACACGGAGAAGTTGGGCTGGTACCACTCGATGGAGGCGG TGCGCAGGGCCTTCCGCGTGTGGGAGCAGGCCACGCCCCTGGTCTTCCAGGAGGTGCCCTATGAGGACAT CCGGCTGCGGCGACAGAAGGAGGCCGACATCATGGTACTCTTTGCCTCTGGCTTCCACGGCGACAGCTCG CCGTTTGATGGCACCGGTGGCTTTCTGGCCCACGCCTATTTCCCTGGCCCCGGCCTAGGCGGGGACACCC ATTTTGACGCAGATGAGCCCTGGACCTTCTCCAGCACTGACCTGCATGGAAACAACCTCTTCCTGGTGGC AGTGCATGAGCTGGGCCACGCGCTGGGGCTGGAGCACTCCAGCAACCCCAATGCCATCATGGCACCGTTC TACCAGTGGAAGGACGTTGACAACTTCAAGCTGCCCGAGGACGATCTCCGTGGCATCCAGCAGCTCTACG GTACCCCAGACGGTCAGCCACAGCCTACCCAGCCTCTCCCCACTGTGACGCCACGGCGGCCAGGCCGGCC TGACCACCGGCCGCCCCGGCCTCCCCAGCCACCACCCCCAGGTGGGAAGCCAGAGCGGCCCCCAAAGCCG GGCCCTCCAGTCCAGCCCCGAGCCACAGAGCGGCCCGACCAGTATGGCCCCAACATCTGCGACGGGGACT TTGACACAGTGGCCATGCTTCGCGGGGAGATGTTCGTGTTCAAGGGCCGCTGGTTCTGGCGAGTCCGGCA CAACCGCGTCCTGGACAACTATCCCATGCCCATCGGGCACTTCTGGCGTGGTCTGCCCGGTGACATCAGT GCTGCCTACGAGCGCCAAGACGGTCGTTTTGTCTTTTTCAAAGGTGACCGCTACTGGCTCTTTCGAGAAG CGAACCTGGAGCCCGGCTACCCACAGCCGCTGACCAGCTATGGCCTGGGCATCCCCTATGACCGCATTGA CACGGCCATCTGGTGGGAGCCCACAGGCCACACCTTCTTCTTCCAAGAGGACAGGTACTGGCGCTTCAAC GAGGAGACACAGCGTGGAGACCCTGGGTACCCCAAGCCCATCAGTGTCTGGCAGGGGATCCCTGCCTCCC CTAAAGGGGCCTTCCTGAGCAATGACGCAGCCTACACCTACTTCTACAAGGGCACCAAATACTGGAAATT CGACAATGAGCGCCTGCGGATGGAGCCCGGCTACCCCAAGTCCATCCTGCGGGACTTCATGGGCTGCCAG GAGCACGTGGAGCCAGGCCCCCGATGGCCCGACGTGGCCCGGCCGCCCTTCAACCCCCACGGGGGTGCAG AGCCCGGGGCGGACAGCGCAGAGGGCGACGTGGGGGATGGGGATGGGGACTTTGGGGCCGGGGTCAACAA GGACGGGGGCAGCCGCGTGGTGGTGCAGATGGAGGAGGTGGCACGGACGGTGAACGTGGTGATGGTGCTG GTGCCACTGCTGCTGCTGCTCTGCGTCCTGGGCCTCACCTACGCGCTGGTGCAGATGCAGCGCAAGGGTG CGCCACGTGTCCTGCTTTACTGCAAGCGCTCGCTGCAGGAGTGGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207318 protein sequence
Red=Cloning site Green=Tags(s) MGSDPSAPGRPGWTGSLLGDREEAARPRLLPLLLVLLGCLGLGVAAEDAEVHAENWLRLYGYLPQPSRHM STMRSAQILASALAEMQRFYGIPVTGVLDEETKEWMKRPRCGVPDQFGVRVKANLRRRRKRYALTGRKWN NHHLTFSIQNYTEKLGWYHSMEAVRRAFRVWEQATPLVFQEVPYEDIRLRRQKEADIMVLFASGFHGDSS PFDGTGGFLAHAYFPGPGLGGDTHFDADEPWTFSSTDLHGNNLFLVAVHELGHALGLEHSSNPNAIMAPF YQWKDVDNFKLPEDDLRGIQQLYGTPDGQPQPTQPLPTVTPRRPGRPDHRPPRPPQPPPPGGKPERPPKP GPPVQPRATERPDQYGPNICDGDFDTVAMLRGEMFVFKGRWFWRVRHNRVLDNYPMPIGHFWRGLPGDIS AAYERQDGRFVFFKGDRYWLFREANLEPGYPQPLTSYGLGIPYDRIDTAIWWEPTGHTFFFQEDRYWRFN EETQRGDPGYPKPISVWQGIPASPKGAFLSNDAAYTYFYKGTKYWKFDNERLRMEPGYPKSILRDFMGCQ EHVEPGPRWPDVARPPFNPHGGAEPGADSAEGDVGDGDGDFGAGVNKDGGSRVVVQMEEVARTVNVVMVL VPLLLLLCVLGLTYALVQMQRKGAPRVLLYCKRSLQEWV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002428 |
| ORF Size | 2007 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002428.4 |
| RefSeq Size | 4456 bp |
| RefSeq ORF | 2010 bp |
| Locus ID | 4324 |
| UniProt ID | P51511 |
| Domains | hemopexin, Peptidase_M10, ZnMc |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| MW | 75.8 kDa |
| Gene Summary | This gene encodes a member of the peptidase M10 family and membrane-type subfamily of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Members of this subfamily contain a transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. The encoded preproprotein is proteolytically processed to generate the mature protease. This protein may play a role in cancer progression. [provided by RefSeq, Jan 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207318L1 | Lenti ORF clone of Human matrix metallopeptidase 15 (membrane-inserted) (MMP15), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC207318L2 | Lenti ORF clone of Human matrix metallopeptidase 15 (membrane-inserted) (MMP15), mGFP tagged |
CNY 7,700.00 |
|
| RC207318L3 | Lenti ORF clone of Human matrix metallopeptidase 15 (membrane-inserted) (MMP15), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC207318L4 | Lenti ORF clone of Human matrix metallopeptidase 15 (membrane-inserted) (MMP15), mGFP tagged |
CNY 7,700.00 |
|
| RG207318 | MMP15 (tGFP-tagged) - Human matrix metallopeptidase 15 (membrane-inserted) (MMP15) |
CNY 6,504.00 |
|
| SC118648 | MMP15 (untagged)-Human matrix metallopeptidase 15 (membrane-inserted) (MMP15) |
CNY 4,912.00 |
