ZNF8 (NM_021089) Human Tagged ORF Clone

CAT#: RC207338

ZNF8 (Myc-DDK-tagged)-Human zinc finger protein 8 (ZNF8)

ORF Plasmid: DDK tGFP



  "NM_021089" in other vectors (4)

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CNY 4,280.00

CNY 4,560.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


ZNF8 rabbit polyclonal antibody
    • 25 ul

CNY 800.00
CNY 1,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms HF.18; Zfp128
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207338 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCCCGAGGACGAAGGGGTAGCGGGAGTGATGTCTGTGGGGCCGCCGGCGGCCCGGCTTCAGGAAC
CAGTGACCTTCCGGGATGTGGCTGTGGACTTTACCCAGGAGGAATGGGGGCAGCTGGACCCTACCCAGAG
GATCCTCTACCGTGACGTGATGCTGGAGACCTTTGGTCACCTGCTCTCCATAGGTCCTGAGCTTCCGAAG
CCTGAAGTCATCTCCCAGCTGGAGCAAGGGACCGAGCTATGGGTGGCTGAGAGAGGAACCACCCAGGGCT
GCCATCCAGCCTGGGAGCCTCGATCTGAAAGCCAAGCATCACGCAAGGAAGAGGGCCTGCCTGAAGAGGA
GCCATCCCATGTCACGGGAAGGGAAGGATTCCCGACAGATGCTCCTTATCCCACCACGTTAGGGAAAGAC
AGGGAGTGTCAGAGCCAGAGTCTGGCACTCAAGGAGCAGAATAACTTGAAGCAGTTGGAATTTGGCCTCA
AGGAAGCACCAGTTCAAGATCAAGGCTACAAAACTCTCAGACTCAGGGAAAACTGCGTCCTGAGTTCAAG
CCCAAATCCATTCCCAGAGATCTCTAGAGGGGAGTATTTGTATACTTACGACTCACAGATTACAGACTCA
GAACATAACTCCAGCTTAGTCAGTCAGCAGACAGGCTCCCCAGGAAAACAGCCCGGTGAAAACAGTGACT
GTCACAGAGATTCCAGTCAGGCCATTCCAATTACGGAACTCACAAAAAGCCAGGTGCAGGACAAACCCTA
CAAATGTACTGACTGTGGGAAGTCGTTTAACCATAACGCACACCTCACCGTGCACAAGAGGATTCATACG
GGAGAAAGACCTTATATGTGCAAGGAGTGTGGGAAAGCCTTCAGCCAGAACTCCTCCCTCGTCCAGCATG
AGCGCATCCACACTGGAGACAAGCCCTACAAGTGTGCCGAATGTGGGAAGTCTTTCTGCCATAGTACACA
CCTTACCGTCCATCGGAGGATTCACACTGGGGAGAAGCCCTATGAGTGTCAGGACTGTGGGAGGGCCTTC
AACCAGAACTCCTCCCTGGGGCGGCACAAGAGGACACACACTGGGGAGAAGCCATACACCTGCAGTGTGT
GTGGGAAATCCTTCTCTCGGACCACTTGCCTTTTCCTGCACCTGAGAACTCACACCGAGGAGAGGCCCTA
CGAGTGTAACCACTGCGGGAAGGGCTTCAGGCACAGCTCATCCCTGGCCCAGCACCAGCGGAAGCACGCG
GGGGAGAAGCCCTTTGAGTGCCGCCAGAGGCTGATCTTTGAGCAGACGCCAGCTCTCACAAAGCATGAAT
GGACAGAAGCCCTGGGCTGTGACCCACCTTTGAGTCAAGATGAGAGGACTCACCGAAGCGACAGACCCTT
CAAATGTAATCAGTGTGGGAAGTGTTTCATTCAGAGCTCTCACCTCATCCGGCACCAGATAACTCACACC
AGAGAGGAGCAGCCCCATGGGCGAAGCCGGCGGCGTGAACAGTCCTCGAGCAGGAACTCACACCTGGTTC
AGCATCAACACCCGAACTCCAGAAAGAGCTCTGCAGGCGGAGCAAAGGCAGGGCAGCCGGAAAGCAGAGC
CCTGGCTTTGTTTGACATCCAAAAAATCATGCAAGAGAAAAACCCTGTGCACGTTATTGGGGTGGAAGAG
CCTTCTGTGGGTGCTTCCATGTTATTTGACATCAGAGAATCCACA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207338 protein sequence
Red=Cloning site Green=Tags(s)

MDPEDEGVAGVMSVGPPAARLQEPVTFRDVAVDFTQEEWGQLDPTQRILYRDVMLETFGHLLSIGPELPK
PEVISQLEQGTELWVAERGTTQGCHPAWEPRSESQASRKEEGLPEEEPSHVTGREGFPTDAPYPTTLGKD
RECQSQSLALKEQNNLKQLEFGLKEAPVQDQGYKTLRLRENCVLSSSPNPFPEISRGEYLYTYDSQITDS
EHNSSLVSQQTGSPGKQPGENSDCHRDSSQAIPITELTKSQVQDKPYKCTDCGKSFNHNAHLTVHKRIHT
GERPYMCKECGKAFSQNSSLVQHERIHTGDKPYKCAECGKSFCHSTHLTVHRRIHTGEKPYECQDCGRAF
NQNSSLGRHKRTHTGEKPYTCSVCGKSFSRTTCLFLHLRTHTEERPYECNHCGKGFRHSSSLAQHQRKHA
GEKPFECRQRLIFEQTPALTKHEWTEALGCDPPLSQDERTHRSDRPFKCNQCGKCFIQSSHLIRHQITHT
REEQPHGRSRRREQSSSRNSHLVQHQHPNSRKSSAGGAKAGQPESRALALFDIQKIMQEKNPVHVIGVEE
PSVGASMLFDIREST

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_021089
ORF Size 1725 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_021089.3
RefSeq Size 2210 bp
RefSeq ORF 1728 bp
Locus ID 7554
UniProt ID P17098
MW 65 kDa
Gene Summary Transcriptional repressor. May modulate BMP and TGF-beta signal transduction, through its interaction with SMAD proteins.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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