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Scramblase 1 (PLSCR1) (NM_021105) Human Tagged ORF Clone
CAT＃: RC207485
PLSCR1 (Myc-DDK-tagged)-Human phospholipid scramblase 1 (PLSCR1)
ORF Plasmid: tGFP
"NM_021105" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MMTRA1B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207485 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAAACAAAACTCACAGATGAATGCTTCTCACCCGGAAACAAACTTGCCAGTTGGGTATCCTCCTC AGTATCCACCGACAGCATTCCAAGGACCTCCAGGATATAGTGGCTACCCTGGGCCCCAGGTCAGCTACCC ACCCCCACCAGCCGGCCATTCAGGTCCTGGCCCAGCTGGCTTTCCTGTCCCAAATCAGCCAGTGTATAAT CAGCCAGTATATAATCAGCCAGTTGGAGCTGCAGGGGTACCATGGATGCCAGCGCCACAGCCTCCATTAA ACTGTCCACCTGGATTAGAATATTTAAGTCAGATAGATCAGATACTGATTCATCAGCAAATTGAACTTCT GGAAGTTTTAACAGGTTTTGAAACTAATAACAAATATGAAATTAAGAACAGCTTTGGACAGAGGGTTTAC TTTGCAGCGGAAGATACTGATTGCTGTACCCGAAATTGCTGTGGGCCATCTAGACCTTTTACCTTGAGGA TTATTGATAATATGGGTCAAGAAGTCATAACTCTGGAGAGACCACTAAGATGTAGCAGCTGTTGTTGTCC CTGCTGCCTTCAGGAGATAGAAATCCAAGCTCCTCCTGGTGTACCAATAGGTTATGTTATTCAGACTTGG CACCCATGTCTACCAAAGTTTACAATTCAAAATGAGAAAAGAGAGGATGTACTAAAAATAAGTGGTCCAT GTGTTGTGTGCAGCTGTTGTGGAGATGTTGATTTTGAGATTAAATCTCTTGATGAACAGTGTGTGGTTGG CAAAATTTCCAAGCACTGGACTGGAATTTTGAGAGAGGCATTTACAGACGCTGATAACTTTGGAATCCAG TTCCCTTTAGACCTTGATGTTAAAATGAAAGCTGTAATGATTGGTGCCTGTTTCCTCATTGACTTCATGT TTTTTGAAAGCACTGGCAGCCAGGAACAAAAATCAGGAGTGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207485 protein sequence
Red=Cloning site Green=Tags(s) MDKQNSQMNASHPETNLPVGYPPQYPPTAFQGPPGYSGYPGPQVSYPPPPAGHSGPGPAGFPVPNQPVYN QPVYNQPVGAAGVPWMPAPQPPLNCPPGLEYLSQIDQILIHQQIELLEVLTGFETNNKYEIKNSFGQRVY FAAEDTDCCTRNCCGPSRPFTLRIIDNMGQEVITLERPLRCSSCCCPCCLQEIEIQAPPGVPIGYVIQTW HPCLPKFTIQNEKREDVLKISGPCVVCSCCGDVDFEIKSLDEQCVVGKISKHWTGILREAFTDADNFGIQ FPLDLDVKMKAVMIGACFLIDFMFFESTGSQEQKSGVW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021105 |
| ORF Size | 954 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021105.3 |
| RefSeq Size | 2228 bp |
| RefSeq ORF | 957 bp |
| Locus ID | 5359 |
| UniProt ID | O15162 |
| Domains | Scramblase |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| MW | 35 kDa |
| Gene Summary | May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Identification and characterization of the novel nuclease activity of human phospholipid scramblase 1
,Sivagnanam, U;Narayana Murthy, S;Gummadi, SN;,
BMC Biochem.
,PubMed ID 27206388
[Scramblase 1]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207485L1 | Lenti ORF clone of Human phospholipid scramblase 1 (PLSCR1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC207485L2 | Lenti ORF clone of Human phospholipid scramblase 1 (PLSCR1), mGFP tagged |
CNY 5,890.00 |
|
| RC207485L3 | Lenti ORF clone of Human phospholipid scramblase 1 (PLSCR1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207485L4 | Lenti ORF clone of Human phospholipid scramblase 1 (PLSCR1), mGFP tagged |
CNY 5,890.00 |
|
| RG207485 | PLSCR1 (tGFP-tagged) - Human phospholipid scramblase 1 (PLSCR1) |
CNY 4,000.00 |
|
| SC321738 | PLSCR1 (untagged)-Human phospholipid scramblase 1 (PLSCR1) |
CNY 2,400.00 |
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