GNMT (NM_018960) Human Tagged ORF Clone
CAT#: RC207497
GNMT (Myc-DDK-tagged)-Human glycine N-methyltransferase (GNMT)
ORF Plasmid: tGFP
"NM_018960" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HEL-S-182mP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207497 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGACAGCGTGTACCGGACCCGCTCCCTGGGGGTGGCGGCCGAAGGGCTCCCGGACCAGTACGCGG ACGGGGAGGCGGCGCGCGTGTGGCAGCTGTATATCGGAGACACCCGCAGCCGCACCGCCGAGTACAAGGC ATGGCTGCTTGGGCTGCTGCGCCAGCACGGCTGCCAGCGGGTGCTCGACGTAGCCTGTGGCACTGGGGTG GACTCCATTATGCTGGTGGAAGAGGGCTTCAGTGTGACGAGTGTGGATGCCAGTGACAAGATGCTGAAGT ATGCACTTAAGGAGCGCTGGAACCGGCGGCACGAGCCCGCCTTCGACAAGTGGGTCATCGAAGAAGCCAA CTGGATGACTCTGGACAAAGATGTGCCCCAGTCAGCAGAGGGTGGCTTTGATGCTGTCATCTGCCTTGGA AACAGTTTCGCTCACTTGCCAGACTGCAAAGGGGACCAGAGTGAGCACCGGCTGGCGCTGAAAAACATTG CGAGCATGGTGCGGGCAGGGGGCCTACTGGTCATTGATCATCGCAACTACGACCACATCCTCAGTACAGG CTGTGCACCCCCAGGGAAGAACATCTACTATAAGAGTGACTTGACCAAGGACGTCACAACATCAGTGCTG ATAGTGAACAACAAGGCCCACATGGTGACCCTGGACTATACGGTGCAGGTGCCGGGGGCTGGCCAGGATG GCTCTCCTGGCTTGAGTAAGTTCCGGCTCTCCTACTACCCACACTGTCTGGCATCCTTCACGGAGCTGCT CCAAGCAGCCTTCGGAGGTAAGTGCCAGCACAGCGTCCTGGGCGACTTCAAGCCTTACAAGCCAGGCCAA ACCTACATTCCCTGCTACTTCATCCACGTGCTCAAGAGGACAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207497 protein sequence
Red=Cloning site Green=Tags(s) MVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCQRVLDVACGTGV DSIMLVEEGFSVTSVDASDKMLKYALKERWNRRHEPAFDKWVIEEANWMTLDKDVPQSAEGGFDAVICLG NSFAHLPDCKGDQSEHRLALKNIASMVRAGGLLVIDHRNYDHILSTGCAPPGKNIYYKSDLTKDVTTSVL IVNNKAHMVTLDYTVQVPGAGQDGSPGLSKFRLSYYPHCLASFTELLQAAFGGKCQHSVLGDFKPYKPGQ TYIPCYFIHVLKRTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_018960 |
ORF Size | 885 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018960.6 |
RefSeq Size | 1091 bp |
RefSeq ORF | 888 bp |
Locus ID | 27232 |
UniProt ID | Q14749 |
Protein Families | Druggable Genome |
Protein Pathways | Glycine, serine and threonine metabolism |
MW | 32.7 kDa |
Gene Summary | The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. This protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). Alternative splicing results in multiple transcript variants. Naturally occurring readthrough transcription occurs between the upstream CNPY3 (canopy FGF signaling regulator 3) gene and this gene and is represented with GeneID:107080644. [provided by RefSeq, Jan 2016] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Signature molecules expressed differentially in a liver disease stage-specific manner by HIV-1 and HCV co-infection
,Whitmill, A;Kim, S;Rojas, V;Gulraiz, F;Afreen, K;Jain, M;Singh, M;Park, IW;,
PLoS ONE
,PubMed ID 30138348
[GNMT]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207497L1 | Lenti ORF clone of Human glycine N-methyltransferase (GNMT), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC207497L2 | Lenti ORF clone of Human glycine N-methyltransferase (GNMT), mGFP tagged |
CNY 5,890.00 |
|
RC207497L3 | Lenti ORF clone of Human glycine N-methyltransferase (GNMT), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC207497L4 | Lenti ORF clone of Human glycine N-methyltransferase (GNMT), mGFP tagged |
CNY 5,890.00 |
|
RG207497 | GNMT (tGFP-tagged) - Human glycine N-methyltransferase (GNMT) |
CNY 4,000.00 |
|
SC125540 | GNMT (untagged)-Human glycine N-methyltransferase (GNMT) |
CNY 2,400.00 |