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Encephalopsin (OPN3) (NM_014322) Human Tagged ORF Clone
CAT＃: RC207753
OPN3 (Myc-DDK-tagged)-Human opsin 3 (OPN3)
ORF Plasmid: tGFP
"NM_014322" in other vectors (5)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ECPN; PPP1R116 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207753 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACTCGGGGAACCGCAGCGGCGGCCACGGCTACTGGGACGGCGGCGGGGCCGCGGGCGCTGAGGGGC CGGCGCCGGCGGGGACACTGAGCCCCGCGCCCCTCTTCAGCCCCGGCACCTACGAGCGCCTGGCGCTGCT GCTGGGCTCCATTGGGCTGCTGGGCGTCGGCAACAACCTGCTGGTGCTCGTCCTCTACTACAAGTTCCAG CGGCTCCGCACTCCCACTCACCTCCTCCTGGTCAACATCAGCCTCAGCGACCTGCTGGTGTCCCTCTTCG GGGTCACCTTTACCTTCGTGTCCTGCCTGAGGAACGGCTGGGTGTGGGACACCGTGGGCTGCGTGTGGGA CGGGTTTAGCGGCAGCCTCTTCGGGATTGTTTCCATTGCCACCCTAACCGTGCTGGCCTATGAACGTTAC ATTCGCGTGGTCCATGCCAGAGTGATCAATTTTTCCTGGGCCTGGAGGGCCATTACCTACATCTGGCTCT ACTCACTGGCGTGGGCAGGAGCACCTCTCCTGGGATGGAACAGGTACATCCTGGACGTACACGGACTAGG CTGCACTGTGGACTGGAAATCCAAGGATGCCAACGATTCCTCCTTTGTGCTTTTCTTATTTCTTGGCTGC CTGGTGGTGCCCCTGGGTGTCATAGCCCATTGCTATGGCCATATTCTATATTCCATTCGAATGCTTCGTT GTGTGGAAGATCTTCAGACAATTCAAGTGATCAAGATTTTAAAATATGAAAAGAAACTGGCCAAAATGTG CTTTTTAATGATATTCACCTTCCTGGTCTGTTGGATGCCTTATATCGTGATCTGCTTCTTGGTGGTTAAT GGTCATGGTCACCTGGTCACTCCAACAATATCTATTGTTTCGTACCTCTTTGCTAAATCGAACACTGTAT ACAATCCAGTGATTTATGTCTTCATGATCAGAAAGTTTCGAAGATCCCTTTTGCAGCTTCTGTGCCTCCG ACTGCTGAGGTGCCAGAGGCCTGCTAAAGACCTACCAGCAGCTGGAAGTGAAATGCAGATCAGACCCATT GTGATGTCACAGAAAGATGGGGACAGGCCAAAGAAAAAAGTGACTTTCAACTCTTCTTCCATCATTTTTA TCATCACCAGTGATGAATCACTGTCAGTTGACGACAGCGACAAAACCAATGGGTCCAAAGTTGATGTAAT CCAAGTTCGTCCTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207753 protein sequence
Red=Cloning site Green=Tags(s) MYSGNRSGGHGYWDGGGAAGAEGPAPAGTLSPAPLFSPGTYERLALLLGSIGLLGVGNNLLVLVLYYKFQ RLRTPTHLLLVNISLSDLLVSLFGVTFTFVSCLRNGWVWDTVGCVWDGFSGSLFGIVSIATLTVLAYERY IRVVHARVINFSWAWRAITYIWLYSLAWAGAPLLGWNRYILDVHGLGCTVDWKSKDANDSSFVLFLFLGC LVVPLGVIAHCYGHILYSIRMLRCVEDLQTIQVIKILKYEKKLAKMCFLMIFTFLVCWMPYIVICFLVVN GHGHLVTPTISIVSYLFAKSNTVYNPVIYVFMIRKFRRSLLQLLCLRLLRCQRPAKDLPAAGSEMQIRPI VMSQKDGDRPKKKVTFNSSSIIFIITSDESLSVDDSDKTNGSKVDVIQVRPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014322 |
| ORF Size | 1206 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014322.3 |
| RefSeq Size | 2632 bp |
| RefSeq ORF | 1209 bp |
| Locus ID | 23596 |
| UniProt ID | Q9H1Y3 |
| Domains | 7tm_1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| MW | 44.9 kDa |
| Gene Summary | Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. In addition to the visual opsins, mammals possess several photoreceptive non-visual opsins that are expressed in extraocular tissues. This gene, opsin 3, is strongly expressed in brain and testis and weakly expressed in liver, placenta, heart, lung, skeletal muscle, kidney, and pancreas. The gene may also be expressed in the retina. The protein has the canonical features of a photoreceptive opsin protein. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207753L3 | Lenti ORF clone of Human opsin 3 (OPN3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207753L4 | Lenti ORF clone of Human opsin 3 (OPN3), mGFP tagged |
CNY 5,890.00 |
|
| RG207753 | OPN3 (tGFP-tagged) - Human opsin 3 (OPN3) |
CNY 5,256.00 |
|
| SC115039 | OPN3 (untagged)-Human opsin 3 (OPN3) |
CNY 3,656.00 |
|
| SC322586 | OPN3 (untagged)-Human opsin 3 (OPN3) |
CNY 3,656.00 |
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