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G protein alpha 13 (GNA13) (NM_006572) Human Tagged ORF Clone
CAT＃: RC207762
- TrueORF®
GNA13 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), alpha 13 (GNA13)
ORF Plasmid: tGFP
"NM_006572" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | G13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207762 representing NM_006572.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGACTTCCTGCCGTCGCGGTCCGTGCTGTCCGTGTGCTTCCCCGGCTGCCTGCTGACGAGTGGC GAGGCCGAGCAGCAACGCAAGTCCAAGGAGATCGACAAATGCCTGTCTCGGGAAAAGACCTATGTGAAG CGGCTGGTGAAGATCCTGCTGCTGGGCGCGGGCGAGAGCGGCAAGTCCACCTTCCTGAAGCAGATGCGG ATCATCCACGGGCAGGACTTCGACCAGCGCGCGCGCGAGGAGTTCCGCCCCACCATCTACAGCAACGTG ATCAAAGGTATGAGGGTGCTGGTTGATGCTCGAGAGAAGCTTCATATTCCCTGGGGAGACAACTCAAAC CAACAACATGGAGATAAGATGATGTCGTTTGATACCCGGGCCCCCATGGCAGCCCAAGGAATGGTGGAA ACAAGGGTTTTCTTACAATATCTTCCTGCTATAAGAGCATTATGGGCAGACAGCGGCATACAGAATGCC TATGACCGGCGTCGAGAATTTCAACTGGGTGAATCTGTAAAATATTTCCTGGATAACTTGGATAAACTT GGAGAACCAGATTATATTCCATCACAACAAGATATTCTGCTTGCCAGAAGACCCACCAAAGGCATCCAT GAATACGACTTTGAAATAAAAAATGTTCCTTTCAAAATGGTTGATGTAGGTGGTCAGAGATCAGAAAGG AAACGTTGGTTTGAATGTTTCGACAGTGTGACATCAATACTTTTCCTTGTTTCCTCAAGTGAATTTGAC CAGGTGCTTATGGAAGATCGACTGACCAATCGCCTTACAGAGTCTCTGAACATTTTTGAAACAATCGTC AATAACCGGGTTTTCAGCAATGTCTCCATAATTCTGTTCTTAAACAAGACAGACTTGCTTGAGGAGAAG GTGCAAATTGTGAGCATCAAAGACTATTTCCTAGAATTTGAAGGGGATCCCCACTGCTTAAGAGACGTC CAAAAATTCCTGGTGGAATGTTTCCGGAACAAACGCCGGGACCAGCAACAGAAGCCCTTATACCACCAC TTCACCACTGCTATCAACACGGAGAACATCCGCCTTGTTTTCCGTGACGTGAAGGATACTATTCTGCAT GACAACCTCAAGCAGCTTATGCTACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC207762
Blue=ORF Red=Cloning site Green=Tag(s) MADFLPSRSVLSVCFPGCLLTSGEAEQQRKSKEIDKCLSREKTYVKRLVKILLLGAGESGKSTFLKQMR IIHGQDFDQRAREEFRPTIYSNVIKGMRVLVDAREKLHIPWGDNSNQQHGDKMMSFDTRAPMAAQGMVE TRVFLQYLPAIRALWADSGIQNAYDRRREFQLGESVKYFLDNLDKLGEPDYIPSQQDILLARRPTKGIH EYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVSSSEFDQVLMEDRLTNRLTESLNIFETIV NNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPHCLRDVQKFLVECFRNKRRDQQQKPLYHH FTTAINTENIRLVFRDVKDTILHDNLKQLMLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006572 |
| ORF Size | 1131 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006572.6 |
| RefSeq Size | 4744 bp |
| RefSeq ORF | 1134 bp |
| Locus ID | 10672 |
| UniProt ID | Q14344 |
| Domains | G-alpha |
| Protein Families | Druggable Genome |
| Protein Pathways | Long-term depression, Regulation of actin cytoskeleton, Vascular smooth muscle contraction |
| MW | 44 kDa |
| Gene Summary | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:15240885, PubMed:16787920, PubMed:16705036, PubMed:27084452). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG) (PubMed:15240885, PubMed:12515866). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway (PubMed:16787920, PubMed:16705036, PubMed:27084452). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed:11976333).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207762L1 | Lenti-ORF clone of GNA13 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), alpha 13 (GNA13) |
CNY 7,888.00 |
|
| RC207762L2 | Lenti-ORF clone of GNA13 (mGFP-tagged)-Human guanine nucleotide binding protein (G protein), alpha 13 (GNA13) |
CNY 5,890.00 |
|
| RC207762L3 | Lenti-ORF clone of GNA13 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), alpha 13 (GNA13) |
CNY 5,890.00 |
|
| RC207762L4 | Lenti-ORF clone of GNA13 (mGFP-tagged)-Human guanine nucleotide binding protein (G protein), alpha 13 (GNA13) |
CNY 5,890.00 |
|
| RG207762 | GNA13 (tGFP-tagged) - Human guanine nucleotide binding protein (G protein), alpha 13 (GNA13) |
CNY 7,088.00 |
|
| SC116002 | GNA13 (untagged)-Human guanine nucleotide binding protein (G protein), alpha 13 (GNA13) |
CNY 5,488.00 |
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