B3GNT1 (B3GNT2) (NM_006577) Human Tagged ORF Clone

Name: B3GNT1 (B3GNT2) (NM_006577) Human Tagged ORF Clone
SKU: RC208100
Price: 3656.00

CAT＃: RC208100

B3GNT2 (Myc-DDK-tagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_006577" in other vectors (7)

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CNY 3,656.00

CNY 3,990.00

货期*

现货

规格

Cited in 1 publication.

Product images

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Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	3-Gn-T1; 3-Gn-T2; B3GN-T2; B3GNT; B3GNT-2; B3GNT1; beta-1; beta3Gn-T1; beta3Gn-T2; BETA3GNT; BGnT-2; BGNT2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC208100 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGTTGGACGTCGAAGAATAAAGTTGTTGGGTATCCTGATGATGGCAAATGTCTTCATTTATTTTA TTATGGAAGTCTCCAAAAGCAGTAGCCAAGAAAAAAATGGAAAAGGGGAAGTAATAATACCCAAAGAGAA GTTCTGGAAGATATCTACCCCTCCCGAGGCATACTGGAACCGAGAGCAAGAGAAGCTGAACCGGCAGTAC AACCCCATCCTGAGCATGCTGACCAACCAGACGGGGGAGGCGGGCAGGCTCTCCAATATAAGCCATCTGA ACTACTGCGAACCTGACCTGAGGGTCACGTCGGTGGTTACGGGTTTTAACAACTTGCCGGACAGATTTAA AGACTTTCTGCTGTATTTGAGATGCCGCAATTATTCACTGCTTATAGATCAGCCGGATAAGTGTGCAAAG AAACCTTTCTTGTTGCTGGCGATTAAGTCCCTCACTCCACATTTTGCCAGAAGGCAAGCAATCCGGGAAT CCTGGGGCCAAGAAAGCAACGCAGGGAACCAAACGGTGGTGCGAGTCTTCCTGCTGGGCCAGACACCCCC AGAGGACAACCACCCCGACCTTTCAGATATGCTGAAATTTGAGAGTGAGAAGCACCAAGACATTCTTATG TGGAACTACAGAGACACTTTCTTCAACTTGTCTCTGAAGGAAGTGCTGTTTCTCAGGTGGGTAAGTACTT CCTGCCCAGACACTGAGTTTGTTTTCAAGGGCGATGACGATGTTTTTGTGAACACCCATCACATCCTGAA TTACTTGAATAGTTTATCCAAGACCAAAGCCAAAGATCTCTTCATAGGTGATGTGATCCACAATGCTGGA CCTCATCGGGATAAGAAGCTGAAGTACTACATCCCAGAAGTTGTTTACTCTGGCCTCTACCCACCCTATG CAGGGGGAGGGGGGTTCCTCTACTCCGGCCACCTGGCCCTGAGGCTGTACCATATCACTGACCAGGTCCA TCTCTACCCCATTGATGACGTTTATACTGGAATGTGCCTTCAGAAACTCGGCCTCGTTCCAGAGAAACAC AAAGGCTTCAGGACATTTGATATCGAGGAGAAAAACAAAAATAACATCTGCTCCTATGTAGATCTGATGT TAGTACATAGTAGAAAACCTCAAGAGATGATTGATATTTGGTCTCAGTTGCAGAGTGCTCATTTAAAATG C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC208100 protein sequence Red=Cloning site Green=Tags(s) MSVGRRRIKLLGILMMANVFIYFIMEVSKSSSQEKNGKGEVIIPKEKFWKISTPPEAYWNREQEKLNRQY NPILSMLTNQTGEAGRLSNISHLNYCEPDLRVTSVVTGFNNLPDRFKDFLLYLRCRNYSLLIDQPDKCAK KPFLLLAIKSLTPHFARRQAIRESWGQESNAGNQTVVRVFLLGQTPPEDNHPDLSDMLKFESEKHQDILM WNYRDTFFNLSLKEVLFLRWVSTSCPDTEFVFKGDDDVFVNTHHILNYLNSLSKTKAKDLFIGDVIHNAG PHRDKKLKYYIPEVVYSGLYPPYAGGGGFLYSGHLALRLYHITDQVHLYPIDDVYTGMCLQKLGLVPEKH KGFRTFDIEEKNKNNICSYVDLMLVHSRKPQEMIDIWSQLQSAHLKC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_006577
ORF Size	1191 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_006577.6
RefSeq Size	2788 bp
RefSeq ORF	1194 bp
Locus ID	10678
UniProt ID	Q9NY97
Domains	Galactosyl_T
Protein Families	Transmembrane
Protein Pathways	Glycosphingolipid biosynthesis - lacto and neolacto series, Keratan sulfate biosynthesis, Metabolic pathways
MW	46 kDa
Gene Summary	This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It prefers the substrate of lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2016]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Structures and mechanism of human glycosyltransferase β1,3-N-acetylglucosaminyltransferase 2 (B3GNT2), an important player in immune homeostasis ,null, The Journal of Biological Chemistry ,PubMed ID 33158990 [B3GNT1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC208100L1	Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,056.00
RC208100L2	Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC208100L3	Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,056.00
RC208100L4	Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG208100	B3GNT2 (tGFP-tagged) - Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,256.00
SC116005	B3GNT2 (untagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2)	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00
SC322639	B3GNT2 (untagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2)	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 7,220.00