United States
Germany
Japan
United Kingdom
China
PCYT1A (NM_005017) Human Tagged ORF Clone
CAT＃: RC208130
PCYT1A (Myc-DDK-tagged)-Human phosphate cytidylyltransferase 1, choline, alpha (PCYT1A)
ORF Plasmid: tGFP
"NM_005017" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CCTA; CCTalpha; CT; CTA; CTPCT; PCYT1; SMDCRD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208130 representing NM_005017
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCACAGTGTTCAGCCAAGGTCAATGCAAGGAAGAGGAGAAAAGAGGCGCCCGGACCCAACGGGG CAACAGAAGAAGATGGGGTTCCTTCCAAAGTGCAGCGCTGTGCAGTGGGCTTACGGCAACCAGCTCCTTT TTCTGATGAAATTGAAGTTGACTTTAGTAAGCCCTATGTCAGGGTAACTATGGAAGAAGCCAGCAGAGGA ACTCCTTGTGAGCGACCTGTGAGAGTTTATGCCGATGGAATATTTGACTTATTTCACTCTGGTCACGCCC GAGCTCTGATGCAAGCGAAGAACCTTTTCCCTAATACGTACCTCATTGTGGGAGTTTGCAGTGATGAGCT CACACACAACTTCAAAGGCTTCACGGTGATGAACGAGAATGAGCGCTATGACGCAGTCCAGCACTGCCGC TACGTGGATGAGGTGGTGAGGAATGCGCCCTGGACGCTGACACCCGAGTTCCTGGCCGAACACCGGATTG ATTTTGTAGCCCATGATGATATTCCTTATTCATCTGCTGGCAGTGATGATGTTTATAAGCACATCAAGGA GGCAGGCATGTTTGCTCCAACACAGAGGACAGAAGGTATCTCCACATCAGACATCATCACCCGAATTGTG CGGGATTATGATGTGTATGCGAGGCGGAACCTGCAGAGGGGCTACACAGCAAAGGAGCTCAATGTCAGCT TTATCAACGAGAAGAAATACCACTTGCAGGAGAGGGTTGACAAAGTAAAGAAGAAAGTGAAAGATGTGGA GGAAAAGTCAAAAGAATTTGTTCAGAAGGTGGAGGAAAAAAGCATTGACCTCATTCAGAAGTGGGAGGAG AAGTCCCGAGAATTCATTGGAAGTTTTCTGGAAATGTTTGGTCCGGAAGGAGCACTGAAACATATGCTGA AAGAGGGGAAGGGCCGGATGCTGCAGGCCATCAGCCCGAAGCAGAGCCCCAGCAGCAGCCCTACTCGCGA GCGCTCCCCCTCCCCCTCTTTCCGATGGCCCTTCTCCGGCAAGACTTCCCCACCTTGCTCCCCAGCAAAT CTCTCCAGGCACAAGGCTGCAGCCTATGATATCAGTGAGGATGAAGAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208130 representing NM_005017
Red=Cloning site Green=Tags(s) MDAQCSAKVNARKRRKEAPGPNGATEEDGVPSKVQRCAVGLRQPAPFSDEIEVDFSKPYVRVTMEEASRG TPCERPVRVYADGIFDLFHSGHARALMQAKNLFPNTYLIVGVCSDELTHNFKGFTVMNENERYDAVQHCR YVDEVVRNAPWTLTPEFLAEHRIDFVAHDDIPYSSAGSDDVYKHIKEAGMFAPTQRTEGISTSDIITRIV RDYDVYARRNLQRGYTAKELNVSFINEKKYHLQERVDKVKKKVKDVEEKSKEFVQKVEEKSIDLIQKWEE KSREFIGSFLEMFGPEGALKHMLKEGKGRMLQAISPKQSPSSSPTRERSPSPSFRWPFSGKTSPPCSPAN LSRHKAAAYDISEDEED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005017 |
| ORF Size | 1101 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005017.4 |
| RefSeq Size | 1677 bp |
| RefSeq ORF | 1104 bp |
| Locus ID | 5130 |
| UniProt ID | P49585 |
| Domains | CTP_transf_2 |
| Protein Pathways | Glycerophospholipid metabolism, Metabolic pathways |
| MW | 41.6 kDa |
| Gene Summary | This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MYC is a positive regulator of choline metabolism and impedes mitophagy-dependent necroptosis in diffuse large B-cell lymphoma
,Xiong, J;Wang, L;Fei, XC;Jiang, XF;Zheng, Z;Zhao, Y;Wang, CF;Li, B;Chen, SJ;Janin, A;Gale, RP;Zhao, WL;,
Blood Cancer J
,PubMed ID 28686226
[PCYT1A]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208130L1 | Lenti ORF clone of Human phosphate cytidylyltransferase 1, choline, alpha (PCYT1A), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208130L2 | Lenti ORF clone of Human phosphate cytidylyltransferase 1, choline, alpha (PCYT1A), mGFP tagged |
CNY 5,890.00 |
|
| RC208130L3 | Lenti ORF clone of Human phosphate cytidylyltransferase 1, choline, alpha (PCYT1A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208130L4 | Lenti ORF clone of Human phosphate cytidylyltransferase 1, choline, alpha (PCYT1A), mGFP tagged |
CNY 5,890.00 |
|
| RG208130 | PCYT1A (tGFP-tagged) - Human phosphate cytidylyltransferase 1, choline, alpha (PCYT1A) |
CNY 4,370.00 |
|
| SC117010 | PCYT1A (untagged)-Human phosphate cytidylyltransferase 1, choline, alpha (PCYT1A) |
CNY 3,656.00 |
