GALNT7 (NM_017423) Human Tagged ORF Clone
CAT#: RC208199
GALNT7 (Myc-DDK-tagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7) (GALNT7)
ORF Plasmid: tGFP
"NM_017423" in other vectors (6)
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CNY 4,896.00
CNY 300.00
CNY 6,281.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GALNAC-T7; GalNAcT7 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC208199 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTGAAGATTGGATTCATCTTACGCAGTTTGCTGGTGGTGGGAAGCTTCCTGGGGCTAGTGGTCC TCTGGTCTTCCCTGACCCCGCGGCCGGACGACCCAAGCCCGCTGAGCAGGATGAGGGAAGACAGAGATGT CAATGACCCCATGCCCAACCGAGGCGGCAATGGACTAGCTCCTGGGGAGGACAGATTCAAACCTGTGGTA CCATGGCCTCATGTTGAAGGAGTAGAAGTGGACTTAGAGTCTATTAGAAGAATAAACAAGGCCAAAAATG AACAAGAGCACCATGCTGGAGGAGATTCCCAGAAAGATATCATGCAGAGGCAGTATCTCACATTTAAGCC TCAGACATTCACCTACCATGATCCTGTGCTTCGCCCAGGGATCCTCGGTAACTTTGAACCCAAAGAACCT GAGCCTCCTGGAGTGGTTGGTGGCCCTGGAGAGAAAGCCAAGCCATTGGTTTTGGGACCAGAATTCAAAC AAGCAATTCAAGCCAGCATTAAAGAGTTTGGATTTAACATGGTGGCAAGTGACATGATCTCACTGGACCG CAGCGTCAATGACTTACGCCAAGAAGAATGCAAGTATTGGCATTATGATGAAAACTTGCTCACTTCGAGC GTTGTCATTGTCTTCCATAATGAAGGATGGTCAACCCTCATGAGAACAGTCCACAGTGTAATTAAAAGGA CTCCAAGGAAATATTTAGCAGAAATTGTGTTAATTGACGATTTCAGTAATAAAGAACACTTAAAAGAAAA ACTGGATGAATATATTAAGCTGTGGAATGGCCTAGTGAAGGTATTTCGAAATGAAAGAAGGGAAGGTTTA ATTCAAGCACGAAGTATTGGTGCTCAGAAGGCTAAACTTGGACAGGTTTTGATATACCTTGATGCCCACT GTGAGGTGGCAGTTAACTGGTATGCACCACTTGTAGCTCCCATATCTAAGGACAGAACCATTTGCACTGT GCCGCTTATAGATGTCATAAATGGCAACACATATGAAATTATACCCCAAGGGGGTGGTGATGAAGATGGG TATGCCCGAGGAGCATGGGATTGGAGTATGCTCTGGAAACGGGTGCCTCTGACCCCTCAAGAGAAGAGAC TGAGAAAGACAAAAACTGAACCGTATCGGTCCCCAGCCATGGCTGGGGGATTATTTGCCATTGAACGAGA GTTCTTCTTTGAATTGGGTCTCTATGATCCAGGTCTCCAGATTTGGGGTGGTGAAAACTTTGAGATCTCA TACAAGATATGGCAGTGTGGTGGCAAATTATTATTTGTTCCTTGTTCTCGTGTTGGACATATCTACCGTC TTGAGGGCTGGCAAGGAAATCCTCCGCCCATTTATGTTGGGTCTTCTCCAACTCTGAAGAATTATGTTAG AGTTGTGGAGGTTTGGTGGGATGAATATAAAGACTACTTCTATGCTAGTCGTCCTGAATCGCAGGCATTA CCATATGGGGATATATCGGAGCTGAAAAAATTTCGAGAAGATCACAACTGCAAAAGTTTTAAGTGGTTCA TGGAAGAAATAGCTTATGATATCACCTCACACTACCCTTTGCCACCCAAAAATGTTGACTGGGGAGAAAT CAGAGGCTTCGAAACTGCTTACTGCATTGATAGCATGGGAAAAACAAATGGAGGCTTTGTTGAACTAGGA CCCTGCCACAGGATGGGAGGGAATCAGCTTTTCAGAATCAATGAAGCAAATCAACTCATGCAGTATGACC AGTGTTTGACAAAGGGAGCTGATGGATCAAAAGTTATGATTACACACTGTAATCTAAATGAATTTAAGGA ATGGCAGTACTTCAAGAACCTGCACAGATTTACTCATATTCCTTCAGGAAAGTGTTTAGATCGCTCAGAG GTCCTGCATCAAGTATTCATCTCCAATTGTGACTCCAGTAAAACGACTCAAAAATGGGAAATGAATAACA TCCATAGTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208199 protein sequence
Red=Cloning site Green=Tags(s) MRLKIGFILRSLLVVGSFLGLVVLWSSLTPRPDDPSPLSRMREDRDVNDPMPNRGGNGLAPGEDRFKPVV PWPHVEGVEVDLESIRRINKAKNEQEHHAGGDSQKDIMQRQYLTFKPQTFTYHDPVLRPGILGNFEPKEP EPPGVVGGPGEKAKPLVLGPEFKQAIQASIKEFGFNMVASDMISLDRSVNDLRQEECKYWHYDENLLTSS VVIVFHNEGWSTLMRTVHSVIKRTPRKYLAEIVLIDDFSNKEHLKEKLDEYIKLWNGLVKVFRNERREGL IQARSIGAQKAKLGQVLIYLDAHCEVAVNWYAPLVAPISKDRTICTVPLIDVINGNTYEIIPQGGGDEDG YARGAWDWSMLWKRVPLTPQEKRLRKTKTEPYRSPAMAGGLFAIEREFFFELGLYDPGLQIWGGENFEIS YKIWQCGGKLLFVPCSRVGHIYRLEGWQGNPPPIYVGSSPTLKNYVRVVEVWWDEYKDYFYASRPESQAL PYGDISELKKFREDHNCKSFKWFMEEIAYDITSHYPLPPKNVDWGEIRGFETAYCIDSMGKTNGGFVELG PCHRMGGNQLFRINEANQLMQYDQCLTKGADGSKVMITHCNLNEFKEWQYFKNLHRFTHIPSGKCLDRSE VLHQVFISNCDSSKTTQKWEMNNIHSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_017423 |
ORF Size | 1971 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_017423.3 |
RefSeq Size | 4339 bp |
RefSeq ORF | 1974 bp |
Locus ID | 51809 |
UniProt ID | Q86SF2 |
Domains | RICIN, Glycos_transf_2 |
Protein Families | Transmembrane |
Protein Pathways | Metabolic pathways, O-Glycan biosynthesis |
MW | 75.4 kDa |
Gene Summary | This gene encodes GalNAc transferase 7, a member of the GalNAc-transferase family. The enzyme encoded by this gene controls the initiation step of mucin-type O-linked protein glycosylation and transfer of N-acetylgalactosamine to serine and threonine amino acid residues. This enzyme is a type II transmembrane protein and shares common sequence motifs with other family members. Unlike other family members, this enzyme shows exclusive specificity for partially GalNAc-glycosylated acceptor substrates and shows no activity with non-glycosylated peptides. This protein may function as a follow-up enzyme in the initiation step of O-glycosylation. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208199L1 | Lenti ORF clone of Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7) (GALNT7), Myc-DDK-tagged |
CNY 7,296.00 |
|
RC208199L2 | Lenti ORF clone of Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7) (GALNT7), mGFP tagged |
CNY 5,890.00 |
|
RC208199L3 | Lenti ORF clone of Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7) (GALNT7), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC208199L4 | Lenti ORF clone of Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7) (GALNT7), mGFP tagged |
CNY 5,890.00 |
|
RG208199 | GALNT7 (tGFP-tagged) - Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7) (GALNT7) |
CNY 6,496.00 |
|
SC114105 | GALNT7 (untagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7) (GALNT7) |
CNY 4,896.00 |