USF2 (NM_003367) Human Tagged ORF Clone

CAT#: RC208339

USF2 (Myc-DDK-tagged)-Human upstream transcription factor 2, c-fos interacting (USF2), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_003367" in other vectors (6)

Need custom modification / cloning service?
Get a free quote

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal anti-USF2 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms bHLHb12; FIP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208339 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACATGCTGGACCCGGGTCTGGATCCCGCTGCCTCGGCCACCGCTGCTGCCGCCGCCAGCCACGACA
AGGGACCCGAGGCGGAGGAGGGCGTCGAGCTGCAGGAAGGCGGGGACGGCCCAGGAGCGGAGGAGCAGAC
AGCGGTGGCCATCACCAGCGTCCAGCAGGCGGCGTTCGGCGACCACAACATCCAGTACCAGTTCCGCACA
GAGACAAATGGAGGACAGGTGACATACCGCGTAGTCCAGGTGACTGATGGTCAGCTGGACGGCCAGGGCG
ACACAGCTGGCGCCGTCAGCGTCGTGTCCACCGCTGCCTTCGCGGGGGGGCAGCAGGCTGTGACCCAGGT
GGGTGTGGACGGGGCAGCCCAGCGCCCGGGCCCCGCCGCTGCCTCTGTGCCCCCAGGTCCTGCAGCGCCC
TTCCCGCTGGCTGTGATCCAAAATCCCTTCAGCAATGGTGGCAGTCCGGCGGCCGAGGCTGTCAGCGGGG
AGGCACGATTTGCCTATTTCCCAGCGTCCAGTGTGGGAGATACTACGGCTGTGTCCGTACAGACCACAGA
CCAGAGCTTGCAGGCTGGAGGCCAGTTCTACGTCATGATGACGCCCCAGGATGTGCTTCAGACAGGAACA
CAGAGGACGATCGCCCCCCGGACACACCCTTACTCTCCAAAAATTGATGGAACCAGAACACCCCGAGATG
AGAGGAGAAGAGCCCAGCACAACGAAGTGGAGCGGAGGCGGAGGGACAAGATCAACAACTGGATCGTCCA
GCTTTCGAAAATCATTCCAGACTGTAACGCAGACAACAGCAAGACGGGAGCGAGTAAAGGAGGGATCCTG
TCCAAGGCCTGCGATTACATCCGGGAGTTGCGCCAGACCAACCAGCGCATGCAGGAGACCTTCAAAGAGG
CCGAGCGGCTGCAGATGGACAACGAGCTCCTGAGGCAGCAGATCGAGGAGCTGAAGAATGAGAACGCCCT
GCTTCGAGCCCAGCTGCAGCAGCACAACCTGGAGATGGTGGGCGAGGGCACCCGGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208339 protein sequence
Red=Cloning site Green=Tags(s)

MDMLDPGLDPAASATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAITSVQQAAFGDHNIQYQFRT
ETNGGQVTYRVVQVTDGQLDGQGDTAGAVSVVSTAAFAGGQQAVTQVGVDGAAQRPGPAAASVPPGPAAP
FPLAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGT
QRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCNADNSKTGASKGGIL
SKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGEGTRQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003367
ORF Size 1038 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003367.4
RefSeq Size 1732 bp
RefSeq ORF 1041 bp
Locus ID 7392
UniProt ID Q15853
Domains HLH
Protein Families Druggable Genome, Transcription Factors
MW 37 kDa
Gene Summary This gene encodes a member of the basic helix-loop-helix leucine zipper family of transcription factors. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs and is involved in regulating multiple cellular processes. [provided by RefSeq, Mar 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...