SPCS3 (NM_021928) Human Tagged ORF Clone
CAT#: RC208380
SPCS3 (Myc-DDK-tagged)-Human signal peptidase complex subunit 3 homolog (S. cerevisiae) (SPCS3)
ORF Plasmid: tGFP
"NM_021928" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | PRO3567; SPC3; SPC22; SPC22/23; SPC23; YLR066W |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC208380 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACACGGTGCTGTCGCGGGCGAACTCACTGTTCGCCTTCTCGCTGAGCGTGATGGCGGCGCTCACCT TCGGCTGCTTCATCACCACCGCCTTCAAAGACAGGAGCGTCCCGGTGCGGCTGCACGTCTCGCGGATCAT GCTAAAAAATGTAGAAGATTTCACTGGACCTAGAGAAAGAAGTGATCTGGGATTTATCACATTTGATATA ACTGCTGATCTAGAGAATATATTTGATTGGAATGTTAAGCAGTTGTTTCTTTATTTATCAGCAGAATATT CAACAAAAAATAATGCTCTGAACCAAGTTGTCCTATGGGACAAGATTGTTTTGAGAGGTGATAATCCGAA GCTGCTGCTGAAAGATATGAAAACAAAATATTTTTTCTTTGACGATGGAAATGGTCTCAAGGGAAACAGG AATGTCACTTTGACCCTGTCTTGGAACGTCGTACCAAATGCTGGAATTCTACCTCTTGTGACAGGATCAG GACACGTATCTGTCCCATTTCCAGATACATATGAAATAACGAAGAGTTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208380 protein sequence
Red=Cloning site Green=Tags(s) MNTVLSRANSLFAFSLSVMAALTFGCFITTAFKDRSVPVRLHVSRIMLKNVEDFTGPRERSDLGFITFDI TADLENIFDWNVKQLFLYLSAEYSTKNNALNQVVLWDKIVLRGDNPKLLLKDMKTKYFFFDDGNGLKGNR NVTLTLSWNVVPNAGILPLVTGSGHVSVPFPDTYEITKSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_021928 |
ORF Size | 540 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021928.4 |
RefSeq Size | 4596 bp |
RefSeq ORF | 543 bp |
Locus ID | 60559 |
UniProt ID | P61009 |
Domains | SPC22 |
Protein Families | Protease, Transmembrane |
MW | 20.3 kDa |
Gene Summary | Component of the microsomal signal peptidase complex which removes signal peptides and other N-terminal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208380L3 | Lenti ORF clone of Human signal peptidase complex subunit 3 homolog (S. cerevisiae) (SPCS3), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC208380L4 | Lenti ORF clone of Human signal peptidase complex subunit 3 homolog (S. cerevisiae) (SPCS3), mGFP tagged |
CNY 5,890.00 |
|
RG208380 | SPCS3 (tGFP-tagged) - Human signal peptidase complex subunit 3 homolog (S. cerevisiae) (SPCS3) |
CNY 4,000.00 |
|
SC127243 | SPCS3 (untagged)-Human signal peptidase complex subunit 3 homolog (S. cerevisiae) (SPCS3) |
CNY 2,400.00 |