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STING (TMEM173) (NM_198282) Human Tagged ORF Clone
CAT＃: RC208418
TMEM173 (Myc-DDK-tagged)-Human transmembrane protein 173 (TMEM173), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_198282" in other vectors (6)
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CNY 5,488.00
| Cited in 5 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ERIS; hMITA; hSTING; MITA; MPYS; NET23; SAVI; STING; STING-beta; TMEM173 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208418 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCACTCCAGCCTGCATCCATCCATCCCGTGTCCCAGGGGTCACGGGGCCCAGAAGGCAGCCTTGG TTCTGCTGAGTGCCTGCCTGGTGACCCTTTGGGGGCTAGGAGAGCCACCAGAGCACACTCTCCGGTACCT GGTGCTCCACCTAGCCTCCCTGCAGCTGGGACTGCTGTTAAACGGGGTCTGCAGCCTGGCTGAGGAGCTG CGCCACATCCACTCCAGGTACCGGGGCAGCTACTGGAGGACTGTGCGGGCCTGCCTGGGCTGCCCCCTCC GCCGTGGGGCCCTGTTGCTGCTGTCCATCTATTTCTACTACTCCCTCCCAAATGCGGTCGGCCCGCCCTT CACTTGGATGCTTGCCCTCCTGGGCCTCTCGCAGGCACTGAACATCCTCCTGGGCCTCAAGGGCCTGGCC CCAGCTGAGATCTCTGCAGTGTGTGAAAAAGGGAATTTCAACGTGGCCCATGGGCTGGCATGGTCATATT ACATCGGATATCTGCGGCTGATCCTGCCAGAGCTCCAGGCCCGGATTCGAACTTACAATCAGCATTACAA CAACCTGCTACGGGGTGCAGTGAGCCAGCGGCTGTATATTCTCCTCCCATTGGACTGTGGGGTGCCTGAT AACCTGAGTATGGCTGACCCCAACATTCGCTTCCTGGATAAACTGCCCCAGCAGACCGGTGACCATGCTG GCATCAAGGATCGGGTTTACAGCAACAGCATCTATGAGCTTCTGGAGAACGGGCAGCGGGCGGGCACCTG TGTCCTGGAGTACGCCACCCCCTTGCAGACTTTGTTTGCCATGTCACAATACAGTCAAGCTGGCTTTAGC CGGGAGGATAGGCTTGAGCAGGCCAAACTCTTCTGCCGGACACTTGAGGACATCCTGGCAGATGCCCCTG AGTCTCAGAACAACTGCCGCCTCATTGCCTACCAGGAACCTGCAGATGACAGCAGCTTCTCGCTGTCCCA GGAGGTTCTCCGGCACCTGCGGCAGGAGGAAAAGGAAGAGGTTACTGTGGGCAGCTTGAAGACCTCAGCG GTGCCCAGTACCTCCACGATGTCCCAAGAGCCTGAGCTCCTCATCAGTGGAATGGAAAAGCCCCTCCCTC TCCGCACGGATTTCTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208418 protein sequence
Red=Cloning site Green=Tags(s) MPHSSLHPSIPCPRGHGAQKAALVLLSACLVTLWGLGEPPEHTLRYLVLHLASLQLGLLLNGVCSLAEEL RHIHSRYRGSYWRTVRACLGCPLRRGALLLLSIYFYYSLPNAVGPPFTWMLALLGLSQALNILLGLKGLA PAEISAVCEKGNFNVAHGLAWSYYIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPD NLSMADPNIRFLDKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFS REDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSA VPSTSTMSQEPELLISGMEKPLPLRTDFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198282 |
| ORF Size | 1137 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_198282.4 |
| RefSeq Size | 2223 bp |
| RefSeq ORF | 1140 bp |
| Locus ID | 340061 |
| UniProt ID | Q86WV6 |
| Protein Pathways | Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway |
| MW | 42.2 kDa |
| Gene Summary | This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
In vitro STING Activation with the cGAMP-STINGΔTM Signaling Complex
,He, Y;Hong, C;Irvine, D;Li, J;Hammond, P;,
BIO-PROTOCOL
,PubMed ID 33732792
[STING]
|
|
MiR-221 negatively regulates innate anti-viral response
,Du, H;Cui, S;Li, Y;Yang, G;Wang, P;Fikrig, E;You, F;,
PLoS ONE
,PubMed ID 30089112
[STING]
|
|
The DNA Sensor, Cyclic GMP-AMP Synthase, Is Essential for Induction of IFN-ß during Chlamydia trachomatis Infection
,Zhang, Y;Yeruva, L;Marinov, A;Prantner, D;Wyrick, PB;Lupashin, V;Nagarajan, UM;,
J. Immunol. July 2014
,PubMed ID 25070851
[STING]
|
|
Cleavage of Interferon Regulatory Factor 7 by Enterovirus 71 3C Suppresses Cellular Responses
,Xiaobo Lei, Xia Xiao, Qinghua Xue, Qi Jin, Bin He, and Jianwei Wang,
J. Virol., Feb 2013; 87: 1690 - 1698.
[STING]
|
|
Stimulator of IFN Gene Is Critical for Induction of IFN-ß during Chlamydia muridarum Infection
,Daniel Prantner, Toni Darville, and Uma M. Nagarajan,
J. Immunol., Mar 2010; 184: 2551 - 2560
[STING]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208418L1 | Lenti ORF clone of Human transmembrane protein 173 (TMEM173), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC208418L2 | Lenti ORF clone of Human transmembrane protein 173 (TMEM173), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 7,888.00 |
|
| RC208418L3 | Lenti ORF clone of Human transmembrane protein 173 (TMEM173), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC208418L4 | Lenti ORF clone of Human transmembrane protein 173 (TMEM173), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 7,888.00 |
|
| RG208418 | TMEM173 (tGFP-tagged) - Human transmembrane protein 173 (TMEM173), nuclear gene encoding mitochondrial protein |
CNY 7,088.00 |
|
| SC321845 | TMEM173 (untagged)-Human transmembrane protein 173 (TMEM173), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
