BAG5 (NM_004873) Human Tagged ORF Clone

CAT#: RC208518

BAG5 (Myc-DDK-tagged)-Human BCL2-associated athanogene 5 (BAG5), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_004873" in other vectors (5)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


BAG5 mouse monoclonal antibody,clone OTI2D6
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms BAG-5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208518 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATATGGGAAACCAACATCCTTCTATTAGTAGGCTTCAGGAAATCCAAAAGGAAGTAAAAAGTGTAG
AACAGCAAGTTATCGGCTTCAGTGGTCTGTCAGATGACAAGAATTACAAGAAACTGGAGAGGATTCTAAC
AAAACAGCTTTTTGAAATAGACTCTGTAGATACTGAAGGAAAAGGAGATATTCAGCAAGCTAGGAAGCGG
GCAGCACAGGAGACAGAACGTCTTCTCAAAGAGTTGGAGCAGAATGCAAACCACCCACACCGGATTGAAA
TACAGAACATTTTTGAGGAAGCCCAGTCCCTCGTGAGAGAGAAAATTGTGCCATTTTATAATGGAGGCAA
CTGCGTAACTGATGAGTTTGAAGAAGGCATCCAAGATATCATTCTGAGGCTGACACATGTTAAAACTGGA
GGAAAAATCTCCTTGCGGAAAGCAAGGTATCACACTTTAACCAAAATCTGGGCGGTGCAAGAGATAATCG
AAGACTGCATGAAAAAGCAGCCTTCCCTGCCGCTTTCCGAGGATGCACATCCTTCCGTTGCCAAAATCAA
CTTCGTGATGTGTGAGGTGAACAAGGCCCGAGGGGTCCTGATTGCACTTCTGATGGGTGTGAACAACAAT
GAGACCTGCAGGCACTTATCCTGTGTGCTCTCGGGGCTGATCGCTGACCTGGATGCTCTAGATGTGTGCG
GCCGGACAGAAATCAGAAATTATCGGAGGGAGGTAGTAGAAGATATCAACAAATTATTGAAATATCTGGA
TTTGGAAGAGGAAGCAGACACAACTAAAGCATTTGACCTGAGACAGAATCATTCCATTTTAAAAATAGAA
AAGGTCCTCAAGAGAATGAGAGAAATAAAAAATGAACTTCTCCAAGCACAAAACCCTTCTGAATTGTACC
TGAGCTCCAAAACAGAATTGCAGGGTTTAATTGGACAGTTGGATGAGGTAAGTCTTGAAAAAAACCCCTG
CATCCGGGAAGCCAGGAGAAGAGCAGTGATCGAGGTGCAAACTCTGATCACATATATTGACTTGAAGGAG
GCCCTTGAGAAAAGAAAGCTGTTTGCTTGTGAGGAGCACCCATCCCATAAAGCCGTCTGGAACGTCCTTG
GAAACTTGTCTGAGATCCAGGGAGAAGTTCTTTCATTTGATGGAAATCGAACCGATAAGAACTACATCCG
GCTGGAAGAGCTGCTCACCAAGCAGCTGCTAGCCCTGGATGCTGTTGATCCGCAGGGAGAAGAGAAGTGT
AAGGCTGCCAGGAAACAAGCTGTGAGGCTTGCGCAGAATATTCTCAGCTATCTCGACCTGAAATCTGATG
AATGGGAGTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208518 protein sequence
Red=Cloning site Green=Tags(s)

MDMGNQHPSISRLQEIQKEVKSVEQQVIGFSGLSDDKNYKKLERILTKQLFEIDSVDTEGKGDIQQARKR
AAQETERLLKELEQNANHPHRIEIQNIFEEAQSLVREKIVPFYNGGNCVTDEFEEGIQDIILRLTHVKTG
GKISLRKARYHTLTKIWAVQEIIEDCMKKQPSLPLSEDAHPSVAKINFVMCEVNKARGVLIALLMGVNNN
ETCRHLSCVLSGLIADLDALDVCGRTEIRNYRREVVEDINKLLKYLDLEEEADTTKAFDLRQNHSILKIE
KVLKRMREIKNELLQAQNPSELYLSSKTELQGLIGQLDEVSLEKNPCIREARRRAVIEVQTLITYIDLKE
ALEKRKLFACEEHPSHKAVWNVLGNLSEIQGEVLSFDGNRTDKNYIRLEELLTKQLLALDAVDPQGEEKC
KAARKQAVRLAQNILSYLDLKSDEWEY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004873
ORF Size 1341 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004873.4
RefSeq Size 4886 bp
RefSeq ORF 1344 bp
Locus ID 9529
UniProt ID Q9UL15
Domains BAG
Protein Families Druggable Genome
MW 51.3 kDa
Gene Summary The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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