TIMELESS (NM_003920) Human Tagged ORF Clone

CAT＃: RC208527

TIMELESS (Myc-DDK-tagged)-Human timeless homolog (Drosophila) (TIMELESS)

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_003920" in other vectors (4)

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CNY 8,408.00

货期*
现货
规格
    • 10 ug
Cited in 1 publication.

Product images

经常一起买 (5)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA335761
Rabbit Polyclonal Anti-TIMELESS Antibody
    • 50 ug

CNY 5,200.00

添加到购物车

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms hTIM; TIM; TIM1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208527 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTTGCACATGATGAACTGTGAACTTCTAGCCACATGTAGTGCCCTTGGGTACTTGGAGGGAGACA
CTTACCATAAGGAACCAGATTGCTTAGAGAGCGTGAAGGATCTCATCCGCTATTTGAGGCATGAGGATGA
GACACGAGATGTGCGGCAGCAGCTGGGGGCAGCCCAGATCCTACAGAGCGACCTTCTGCCCATCCTCACC
CAGCACCACCAGGACAAGCCTCTCTTTGATGCTGTTATCAGACTGATGGTGAACTTGACACAACCAGCCT
TGCTCTGTTTTGGCAATCTGCCTAAGGAGCCCAGCTTTCGGCACCATTTTTTGCAGGTGCTAACTTATTT
GCAGGCCTACAAAGAGGCCTTTGCCAGTGAGAAGGCTTTTGGAGTCCTCAGTGAAACCTTGTATGAGCTG
CTGCAGCTGGGCTGGGAGGAACGGCAGGAGGAAGACAACTTGCTGATTGAACGGATCCTACTGCTGGTCA
GAAATATTCTCCATGTCCCAGCTGACCTTGATCAGGAGAAGAAGATTGATGATGACGCCAGTGCCCATGA
CCAGCTCCTCTGGGCGATTCACCTCAGCGGCCTGGATGACCTGCTCCTCTTTCTGGCCAGCTCGTCTGCT
GAGGAGCAATGGAGCCTACATGTGCTAGAGATTGTCTCCCTTATGTTTCGTGACCAGAACCCCGAGCAGC
TGGCGGGAGTAGGGCAGGGACGCTTAGCTCAGGAGCGGAGTGCAGATTTTGCAGAACTGGAGGTGTTGCG
CCAGCGAGAGATGGCAGAAAAGAAGACTCGAGCCCTCCAGCGAGGCAACAGGCATTCTCGATTTGGGGGC
TCCTATATTGTCCAGGGGTTGAAATCCATTGGGGAGAGGGACCTCATCTTTCACAAAGGCCTTCACAACC
TACGAAACTACAGTTCAGATTTGGGAAAGCAGCCGAAAAAGGTGCCTAAACGTCGCCAGGCCGCCCGAGA
GCTGTCCATTCAGCGCCGTTCTGCCCTCAATGTGAGGCTCTTCCTCAGAGACTTCTGCTCTGAGTTCCTG
GAGAACTGTTACAACCGGCTCATGGGATCAGTAAAGGATCACCTGCTTCGGGAGAAAGCTCAGCAGCATG
ATGAGACCTATTATATGTGGGCCTTGGCTTTCTTCATGGCCTTCAACCGAGCTGCCTCCTTCCGGCCAGG
CCTGGTTTCTGAGACCCTCAGTGTCCGTACCTTCCACTTCATTGAGCAGAACCTCACCAACTACTATGAG
ATGATGCTGACTGACCGCAAGGAAGCTGCCTCCTGGGCACGCCGGATGCACTTGGCTCTGAAGGCCTATC
AGGAGCTGCTGGCAACAGTGAATGAGATGGACATATCTCCAGATGAGGCTGTGAGGGAGAGCAGCCGCAT
CATCAAGAACAATATTTTCTATGTGATGGAGTACCGAGAACTATTCCTGGCACTTTTTCGAAAGTTTGAT
GAGAGATGCCAGCCCCGCTCTTTCCTTCGTGACCTGGTGGAGACCACCCACCTCTTCCTCAAAATGTTGG
AGCGATTCTGTCGGAGCCGTGGGAACCTGGTGGTGCAGAACAAACAAAAGAAGAGAAGGAAGAAGAAGAA
GAAGGTCCTAGACCAGGCCATTGTTTCTGGTAATGTCCCATCTAGCCCAGAAGAAGTGGAGGCTGTGTGG
CCAGCCCTGGCTGAGCAGCTACAGTGCTGTGCCCAGAATTCTGAGCTCAGCATGGACTCCGTGGTTCCCT
TTGATGCGGCCTCAGAGGTGCCAGTGGAGGAGCAGCGGGCAGAAGCTATGGTACGGATCCAAGACTGTCT
CCTGGCTGGCCAGGCCCCACAGGCCCTGACTCTCCTGAGGTCTGCTCGGGAGGTGTGGCCTGAAGGAGAT
GTGTTTGGCTCTCAAGACATTTCTCCAGAGGAAGAGATCCAGTTGCTGAAACAAATCCTCTCTGCTCCAC
TTCCCCGGCAGCAGGGCCCAGAGGAACGTGGGGCAGAGGAAGAAGAAGAGGAGGAGGAGGAGGAAGAGGA
GGAGTTGCAAGTGGTCCAGGTGTCGGAGAAAGAATTTAATTTTCTGGACTACCTGAAACGCTTTGCATGT
TCAACTGTCGTTCGAGCCTATGTGCTGCTACTAAGGAGCTACCAGCAGAATAGTGCCCACACTAACCATT
GCATTGTGAAGATGCTGCACCGGCTGGCCCATGACCTCAAAATGGAAGCCCTACTTTTTCAGCTGTCAGT
CTTCTGCCTCTTCAATCGTCTGCTTAGTGACCCTGCTGCTGGAGCCTACAAAGAGCTAGTGACTTTTGCC
AAATACATCCTGGGCAAATTTTTTGCACTGGCTGCAGTCAACCAAAAAGCCTTTGTGGAGCTGTTGTTCT
GGAAGAACACAGCTGTGGTTCGAGAGATGACTGAGGGCTATGGCTCCCTGGATGACAGGTCTTCCAGTCG
CAGAGCACCTACATGGAGCCCCGAAGAAGAGGCTCATCTTCGGGAGCTGTACCTCGCCAATAAGGACGTG
GAAGGGCAGGATGTGGTGGAAGCCATCTTGGCCCACCTGAATACTGTTCCTCGAACACGCAAGCAGATCA
TCCACCATCTGGTACAGATGGGACTGGCTGACAGTGTCAAGGACTTCCAAAGGAAAGGAACCCATATTGT
ACTGTGGACGGGGGATCAGGAGTTGGAGCTGCAGCGGCTTTTTGAGGAATTCCGGGACTCAGATGATGTC
CTGGGTCATATCATGAAGAATATCACAGCCAAACGCTCACGGGCCCGAATAGTGGATAAACTCTTGGCTC
TGGGGCTGGTGGCTGAGCGGCGGGAGCTGTACAAGAAACGGCAGAAAAAGTTGGCATCCTCCATCTTGCC
AAATGGAGCGGAGTCCCTGAAAGATTTTTGCCAGGAAGATCTGGAAGAAGAGGAAAACCTGCCTGAGGAA
GACAGCGAAGAGGAAGAAGAAGGGGGCTCAGAAGCAGAACAAGTCCAGGGTAGCTTAGTCCTTTCAAATG
AAAACCTTGGTCAAAGCCTGCATCAGGAAGGCTTTTCTATCCTGCTCCTATGGCTCCAGAACTGCCTGAT
CCGAGCAGCTGATGATCGGGAAGAGGATGGCTGCTCCCAGGCCGTTCCATTGGTGCCACTCACAGAGGAA
AATGAGGAAGCCATGGAAAACGAACAGTTTCAGCAGCTGTTGCGCAAGCTAGGGGTTCGGCCCCCTGCCT
CTGGGCAGGAAACCTTCTGGCGAATTCCAGCCAAGCTGAGTCCTACCCAGCTCCGGAGGGCAGCAGCTTC
TTTGAGTCAACCAGAGGAGGAACAGAAGCTGCAGCCAGAGCTGCAGCCTAAAGTCCCTGGAGAGCAAGGC
TCTGATGAGGAGCACTGTAAAGAGCACCGAGCACAAGCCCTGAGGGCCCTCTTGCTAGCCCACAAGAAGA
AAGCGGGCCTGGCATCCCCAGAGGAGGAAGACGCTGTTGGTAAAGAGCCGCTGAAGGCAGCACCCAAGAA
ACGACAATTGCTGGACAGCGACGAGGAACAGGAAGAAGATGAGGGCAGGAACAGAGCACCAGAGTTGGGA
GCTCCAGGAATCCAAAAGAAGAAACGATACCAGATTGAGGATGATGAGGATGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RC208527 protein sequence
Red=Cloning site Green=Tags(s)

MDLHMMNCELLATCSALGYLEGDTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILT
QHHQDKPLFDAVIRLMVNLTQPALLCFGNLPKEPSFRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYEL
LQLGWEERQEEDNLLIERILLLVRNILHVPADLDQEKKIDDDASAHDQLLWAIHLSGLDDLLLFLASSSA
EEQWSLHVLEIVSLMFRDQNPEQLAGVGQGRLAQERSADFAELEVLRQREMAEKKTRALQRGNRHSRFGG
SYIVQGLKSIGERDLIFHKGLHNLRNYSSDLGKQPKKVPKRRQAARELSIQRRSALNVRLFLRDFCSEFL
ENCYNRLMGSVKDHLLREKAQQHDETYYMWALAFFMAFNRAASFRPGLVSETLSVRTFHFIEQNLTNYYE
MMLTDRKEAASWARRMHLALKAYQELLATVNEMDISPDEAVRESSRIIKNNIFYVMEYRELFLALFRKFD
ERCQPRSFLRDLVETTHLFLKMLERFCRSRGNLVVQNKQKKRRKKKKKVLDQAIVSGNVPSSPEEVEAVW
PALAEQLQCCAQNSELSMDSVVPFDAASEVPVEEQRAEAMVRIQDCLLAGQAPQALTLLRSAREVWPEGD
VFGSQDISPEEEIQLLKQILSAPLPRQQGPEERGAEEEEEEEEEEEEELQVVQVSEKEFNFLDYLKRFAC
STVVRAYVLLLRSYQQNSAHTNHCIVKMLHRLAHDLKMEALLFQLSVFCLFNRLLSDPAAGAYKELVTFA
KYILGKFFALAAVNQKAFVELLFWKNTAVVREMTEGYGSLDDRSSSRRAPTWSPEEEAHLRELYLANKDV
EGQDVVEAILAHLNTVPRTRKQIIHHLVQMGLADSVKDFQRKGTHIVLWTGDQELELQRLFEEFRDSDDV
LGHIMKNITAKRSRARIVDKLLALGLVAERRELYKKRQKKLASSILPNGAESLKDFCQEDLEEEENLPEE
DSEEEEEGGSEAEQVQGSLVLSNENLGQSLHQEGFSILLLWLQNCLIRAADDREEDGCSQAVPLVPLTEE
NEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLSPTQLRRAAASLSQPEEEQKLQPELQPKVPGEQG
SDEEHCKEHRAQALRALLLAHKKKAGLASPEEEDAVGKEPLKAAPKKRQLLDSDEEQEEDEGRNRAPELG
APGIQKKKRYQIEDDEDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003920
ORF Size 3624 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003920.5
RefSeq Size 5154 bp
RefSeq ORF 3627 bp
Locus ID 8914
UniProt ID Q9UNS1
Domains TIMELESS, TIMELESS_C
Protein Families Transcription Factors
MW 138.7 kDa
Gene Summary The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders. [provided by RefSeq, Feb 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Spirodela polyrhiza extract modulates the activation of atopic dermatitis-related ion channels, Orai1 and TRPV3, and inhibits mast cell degranulation ,Nam, JH;Jung, HW;Chin, YW;Yang, WM;Bae, HS;Kim, WK;, Pharm Biol ,PubMed ID 28290212 [TIMELESS]

Other Versions

SKU Description Size Price
RC208527L3 Lenti ORF clone of Human timeless homolog (Drosophila) (TIMELESS), Myc-DDK-tagged
    • 10 ug

CNY 13,970.00

RC208527L4 Lenti ORF clone of Human timeless homolog (Drosophila) (TIMELESS), mGFP tagged
    • 10 ug

CNY 13,970.00

RG208527 TIMELESS (tGFP-tagged) - Human timeless homolog (Drosophila) (TIMELESS)
    • 10 ug

CNY 10,008.00

SC117684 TIMELESS (untagged)-Human timeless homolog (Drosophila) (TIMELESS)
    • 10 ug

CNY 8,416.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

其它TIMELESS产品

Expression Plasmids
Lentiviral Particles
CRISPR
RNAi
Proteins
Antibodies
相关产品 (9)

PS100001

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    • 10 ug

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RC208527L3V

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RC208527L4

Lenti ORF clone of Human timeless homolog (Drosophila) (TIMELESS), mGFP tagged

    • 10 ug

CNY 13,970.00

RC208527L3

Lenti ORF clone of Human timeless homolog (Drosophila) (TIMELESS), Myc-DDK-tagged

    • 10 ug

CNY 13,970.00

RG208527

TIMELESS (tGFP-tagged) - Human timeless homolog (Drosophila) (TIMELESS)

    • 10 ug

CNY 10,008.00

RC208527L4V

Lenti ORF particles, TIMELESS (mGFP-tagged) - Human timeless homolog (Drosophila) (TIMELESS), 200ul, >10^7 TU/mL

    • 200 ul

CNY 12,540.00 暂停销售

SC117684

TIMELESS (untagged)-Human timeless homolog (Drosophila) (TIMELESS)

    • 10 ug

CNY 8,416.00

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