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PPP1R16A (NM_032902) Human Tagged ORF Clone
CAT＃: RC208548
PPP1R16A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 16A (PPP1R16A)
ORF Plasmid: tGFP
"NM_032902" in other vectors (6)
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CNY 3,936.00
CNY 3,990.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MYPT3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208548 representing NM_032902
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGCACCTGGAGCTGCTGGCAGAGATGCCCATGGTGGGCAGGATGAGCACACAGGAGCGGCTGA AGCATGCCCAGAAGCGGCGCGCCCAGCAGGTGAAGATGTGGGCCCAGGCTGAGAAGGAGGCCCAGGGCAA GAAGGGTCCTGGGGAGCGTCCCCGGAAGGAGGCAGCCAGCCAAGGGCTCCTGAAGCAGGTCCTCTTCCCT CCCAGTGTTGTCCTTCTGGAGGCCGCTGCCCGAAATGACCTGGAAGAAGTCCGCCAGTTCCTTGGGAGTG GGGTCAGCCCTGACTTGGCCAACGAGGACGGCCTGACGGCCCTGCACCAGTGCTGCATTGATGATTTCCG AGAGATGGTGCAGCAGCTCCTGGAGGCTGGGGCCAACATCAATGCCTGTGACAGTGAGTGCTGGACGCCT CTGCATGCTGCGGCCACCTGCGGCCACCTGCACCTGGTGGAGCTGCTCATCGCCAGTGGCGCCAATCTCC TGGCGGTCAACACCGACGGGAACATGCCCTATGACCTGTGTGATGATGAGCAGACGCTGGACTGCCTGGA GACTGCCATGGCCGACCGTGGCATCACCCAGGACAGCATCGAGGCCGCCCGGGCCGTGCCAGAACTGCGC ATGCTGGACGACATCCGGAGCCGGCTGCAGGCCGGGGCAGACCTCCATGCCCCCCTGGACCACGGGGCCA CGCTGCTGCACGTCGCAGCCGCCAACGGGTTCAGCGAGGCGGCTGCCCTGCTGCTGGAACACCGAGCCAG CCTGAGCGCTAAGGACCAAGACGGCTGGGAGCCGCTGCACGCCGCGGCCTACTGGGGCCAGGTGCCCCTG GTGGAGCTGCTCGTGGCGCACGGGGCCGACCTGAACGCAAAGTCCCTGATGGACGAGACGCCCCTTGATG TGTGCGGGGACGAGGAGGTGCGGGCCAAGCTGCTGGAGCTGAAGCACAAGCACGACGCCCTCCTGCGCGC CCAGAGCCGCCAGCGCTCCTTGCTGCGCCGCCGCACCTCCAGCGCCGGCAGCCGCGGGAAGGTGGTGAGG CGGGTGAGCCTAACCCAGCGCACCGACCTGTACCGCAAGCAGCACGCCCAGGAGGCCATCGTGTGGCAAC AGCCGCCGCCCACCAGCCCGGAGCCGCCCGAGGACAACGATGACCGCCAGACAGGCGCAGAGCTCAGGCC GCCGCCCCCGGAGGAGGACAACCCCGAAGTGGTCAGGCCGCACAATGGCCGAGTAGGGGGCTCCCCAGTG CGGCATCTATACTCCAAGCGACTAGACCGGAGTGTCTCCTACCAGCTGAGCCCCCTGGACAGCACCACCC CCCACACCCTGGTCCACGACAAGGCCCACCACACCCTGGCTGACCTGAAGCGCCAGCGAGCTGCTGCCAA GCTGCAGCGACCCCCACCTGAGGGGCCCGAGAGCCCTGAGACAGCTGAGCCTGGCCTGCCTGGTGACACG GTGACCCCCCAGCCTGACTGTGGCTTCAGGGCAGGCGGGGACCCACCCCTGCTCAAGCTCACAGCCCCGG CGGTGGAGGCTCCCGTGGAGAGGAGGCCGTGCTGCCTGCTCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208548 representing NM_032902
Red=Cloning site Green=Tags(s) MAEHLELLAEMPMVGRMSTQERLKHAQKRRAQQVKMWAQAEKEAQGKKGPGERPRKEAASQGLLKQVLFP PSVVLLEAAARNDLEEVRQFLGSGVSPDLANEDGLTALHQCCIDDFREMVQQLLEAGANINACDSECWTP LHAAATCGHLHLVELLIASGANLLAVNTDGNMPYDLCDDEQTLDCLETAMADRGITQDSIEAARAVPELR MLDDIRSRLQAGADLHAPLDHGATLLHVAAANGFSEAAALLLEHRASLSAKDQDGWEPLHAAAYWGQVPL VELLVAHGADLNAKSLMDETPLDVCGDEEVRAKLLELKHKHDALLRAQSRQRSLLRRRTSSAGSRGKVVR RVSLTQRTDLYRKQHAQEAIVWQQPPPTSPEPPEDNDDRQTGAELRPPPPEEDNPEVVRPHNGRVGGSPV RHLYSKRLDRSVSYQLSPLDSTTPHTLVHDKAHHTLADLKRQRAAAKLQRPPPEGPESPETAEPGLPGDT VTPQPDCGFRAGGDPPLLKLTAPAVEAPVERRPCCLLM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032902 |
| ORF Size | 1584 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032902.7 |
| RefSeq Size | 2326 bp |
| RefSeq ORF | 1587 bp |
| Locus ID | 84988 |
| UniProt ID | Q96I34 |
| Domains | ANK |
| MW | 57.6 kDa |
| Gene Summary | Myosin light chain kinase and phosphatase (MLCP) complexes control the phosphorylation states of regulatory myosin light chains, which is crucial for muscle and intracellular movement. MLCPs typically contain a catalytic protein phosphatase 1 (PP1c) subunit, a myosin phosphatase targeting (MYPT) subunit, and another smaller subunit. The protein encoded by this gene represents an MYPT subunit, which is responsible for directing PP1c to its intended targets. However, while the phosphorylation of other MYPT members results in PP1c inactivation, phosphorylation of the encoded protein by protein kinase A results in PP1c activation. [provided by RefSeq, Jan 2020] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208548L1 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 16A (PPP1R16A), Myc-DDK-tagged |
CNY 6,336.00 |
|
| RC208548L2 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 16A (PPP1R16A), mGFP tagged |
CNY 5,890.00 |
|
| RC208548L3 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 16A (PPP1R16A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208548L4 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 16A (PPP1R16A), mGFP tagged |
CNY 5,890.00 |
|
| RG208548 | PPP1R16A (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 16A (PPP1R16A) |
CNY 4,370.00 |
|
| SC102658 | PPP1R16A (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 16A (PPP1R16A) |
CNY 3,936.00 |
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