PPP1R16A (NM_032902) Human Tagged ORF Clone

CAT#: RC208548

PPP1R16A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 16A (PPP1R16A)

ORF Plasmid: DDK tGFP



  "NM_032902" in other vectors (6)

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CNY 3,936.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal antibody to PPP1R16A (protein phosphatase 1, regulatory (inhibitor) subunit 16A)
    • 100 ul

CNY 6,281.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms MYPT3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208548 representing NM_032902
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGCACCTGGAGCTGCTGGCAGAGATGCCCATGGTGGGCAGGATGAGCACACAGGAGCGGCTGA
AGCATGCCCAGAAGCGGCGCGCCCAGCAGGTGAAGATGTGGGCCCAGGCTGAGAAGGAGGCCCAGGGCAA
GAAGGGTCCTGGGGAGCGTCCCCGGAAGGAGGCAGCCAGCCAAGGGCTCCTGAAGCAGGTCCTCTTCCCT
CCCAGTGTTGTCCTTCTGGAGGCCGCTGCCCGAAATGACCTGGAAGAAGTCCGCCAGTTCCTTGGGAGTG
GGGTCAGCCCTGACTTGGCCAACGAGGACGGCCTGACGGCCCTGCACCAGTGCTGCATTGATGATTTCCG
AGAGATGGTGCAGCAGCTCCTGGAGGCTGGGGCCAACATCAATGCCTGTGACAGTGAGTGCTGGACGCCT
CTGCATGCTGCGGCCACCTGCGGCCACCTGCACCTGGTGGAGCTGCTCATCGCCAGTGGCGCCAATCTCC
TGGCGGTCAACACCGACGGGAACATGCCCTATGACCTGTGTGATGATGAGCAGACGCTGGACTGCCTGGA
GACTGCCATGGCCGACCGTGGCATCACCCAGGACAGCATCGAGGCCGCCCGGGCCGTGCCAGAACTGCGC
ATGCTGGACGACATCCGGAGCCGGCTGCAGGCCGGGGCAGACCTCCATGCCCCCCTGGACCACGGGGCCA
CGCTGCTGCACGTCGCAGCCGCCAACGGGTTCAGCGAGGCGGCTGCCCTGCTGCTGGAACACCGAGCCAG
CCTGAGCGCTAAGGACCAAGACGGCTGGGAGCCGCTGCACGCCGCGGCCTACTGGGGCCAGGTGCCCCTG
GTGGAGCTGCTCGTGGCGCACGGGGCCGACCTGAACGCAAAGTCCCTGATGGACGAGACGCCCCTTGATG
TGTGCGGGGACGAGGAGGTGCGGGCCAAGCTGCTGGAGCTGAAGCACAAGCACGACGCCCTCCTGCGCGC
CCAGAGCCGCCAGCGCTCCTTGCTGCGCCGCCGCACCTCCAGCGCCGGCAGCCGCGGGAAGGTGGTGAGG
CGGGTGAGCCTAACCCAGCGCACCGACCTGTACCGCAAGCAGCACGCCCAGGAGGCCATCGTGTGGCAAC
AGCCGCCGCCCACCAGCCCGGAGCCGCCCGAGGACAACGATGACCGCCAGACAGGCGCAGAGCTCAGGCC
GCCGCCCCCGGAGGAGGACAACCCCGAAGTGGTCAGGCCGCACAATGGCCGAGTAGGGGGCTCCCCAGTG
CGGCATCTATACTCCAAGCGACTAGACCGGAGTGTCTCCTACCAGCTGAGCCCCCTGGACAGCACCACCC
CCCACACCCTGGTCCACGACAAGGCCCACCACACCCTGGCTGACCTGAAGCGCCAGCGAGCTGCTGCCAA
GCTGCAGCGACCCCCACCTGAGGGGCCCGAGAGCCCTGAGACAGCTGAGCCTGGCCTGCCTGGTGACACG
GTGACCCCCCAGCCTGACTGTGGCTTCAGGGCAGGCGGGGACCCACCCCTGCTCAAGCTCACAGCCCCGG
CGGTGGAGGCTCCCGTGGAGAGGAGGCCGTGCTGCCTGCTCATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208548 representing NM_032902
Red=Cloning site Green=Tags(s)

MAEHLELLAEMPMVGRMSTQERLKHAQKRRAQQVKMWAQAEKEAQGKKGPGERPRKEAASQGLLKQVLFP
PSVVLLEAAARNDLEEVRQFLGSGVSPDLANEDGLTALHQCCIDDFREMVQQLLEAGANINACDSECWTP
LHAAATCGHLHLVELLIASGANLLAVNTDGNMPYDLCDDEQTLDCLETAMADRGITQDSIEAARAVPELR
MLDDIRSRLQAGADLHAPLDHGATLLHVAAANGFSEAAALLLEHRASLSAKDQDGWEPLHAAAYWGQVPL
VELLVAHGADLNAKSLMDETPLDVCGDEEVRAKLLELKHKHDALLRAQSRQRSLLRRRTSSAGSRGKVVR
RVSLTQRTDLYRKQHAQEAIVWQQPPPTSPEPPEDNDDRQTGAELRPPPPEEDNPEVVRPHNGRVGGSPV
RHLYSKRLDRSVSYQLSPLDSTTPHTLVHDKAHHTLADLKRQRAAAKLQRPPPEGPESPETAEPGLPGDT
VTPQPDCGFRAGGDPPLLKLTAPAVEAPVERRPCCLLM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032902
ORF Size 1584 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_032902.7
RefSeq Size 2326 bp
RefSeq ORF 1587 bp
Locus ID 84988
UniProt ID Q96I34
Domains ANK
MW 57.6 kDa
Gene Summary Myosin light chain kinase and phosphatase (MLCP) complexes control the phosphorylation states of regulatory myosin light chains, which is crucial for muscle and intracellular movement. MLCPs typically contain a catalytic protein phosphatase 1 (PP1c) subunit, a myosin phosphatase targeting (MYPT) subunit, and another smaller subunit. The protein encoded by this gene represents an MYPT subunit, which is responsible for directing PP1c to its intended targets. However, while the phosphorylation of other MYPT members results in PP1c inactivation, phosphorylation of the encoded protein by protein kinase A results in PP1c activation. [provided by RefSeq, Jan 2020]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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