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CD31 (PECAM1) (NM_000442) Human Tagged ORF Clone
CAT＃: RC208654
PECAM1 (Myc-DDK-tagged)-Human platelet/endothelial cell adhesion molecule (PECAM1)
ORF Plasmid: tGFP
"NM_000442" in other vectors (6)
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CNY 8,104.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208654 representing NM_000442
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCGAGGTGGGCCCAAGGGGCCACGATGTGGCTTGGAGTCCTGCTGACCCTTCTGCTCTGTTCAA GCCTTGAGGGTCAAGAAAACTCTTTCACAATCAACAGTGTTGACATGAAGAGCCTGCCGGACTGGACGGT GCAAAATGGGAAGAACCTGACCCTGCAGTGCTTCGCGGATGTCAGCACCACCTCTCACGTCAAGCCTCAG CACCAGATGCTGTTCTATAAGGATGACGTGCTGTTTTACAACATCTCCTCCATGAAGAGCACAGAGAGTT ATTTTATTCCTGAAGTCCGGATCTATGACTCAGGGACATATAAATGTACTGTGATTGTGAACAACAAAGA GAAAACCACTGCAGAGTACCAGGTGTTGGTGGAAGGAGTGCCCAGTCCCAGGGTGACACTGGACAAGAAA GAGGCCATCCAAGGTGGGATCGTGAGGGTCAACTGTTCTGTCCCAGAGGAAAAGGCCCCAATACACTTCA CAATTGAAAAACTTGAACTAAATGAAAAAATGGTCAAGCTGAAAAGAGAGAAGAATTCTCGAGACCAGAA TTTTGTGATACTGGAATTCCCCGTTGAGGAACAGGACCGCGTTTTATCCTTCCGATGTCAAGCTAGGATC ATTTCTGGGATCCATATGCAGACCTCAGAATCTACCAAGAGTGAACTGGTCACCGTGACGGAATCCTTCT CTACACCCAAGTTCCACATCAGCCCCACCGGAATGATCATGGAAGGAGCTCAGCTCCACATTAAGTGCAC CATTCAAGTGACTCACCTGGCCCAGGAGTTTCCAGAAATCATAATTCAGAAGGACAAGGCGATTGTGGCC CACAACAGACATGGCAACAAGGCTGTGTACTCAGTCATGGCCATGGTGGAGCACAGTGGCAACTACACGT GCAAAGTGGAGTCCAGCCGCATATCCAAGGTCAGCAGCATCGTGGTCAACATAACAGAACTATTTTCCAA GCCCGAACTGGAATCTTCCTTCACACATCTGGACCAAGGTGAAAGACTGAACCTGTCCTGCTCCATCCCA GGAGCACCTCCAGCCAACTTCACCATCCAGAAGGAAGATACGATTGTGTCACAGACTCAAGATTTCACCA AGATAGCCTCAAAGTCGGACAGTGGGACGTATATCTGCACTGCAGGTATTGACAAAGTGGTCAAGAAAAG CAACACAGTCCAGATAGTCGTATGTGAAATGCTCTCCCAGCCCAGGATTTCTTATGATGCCCAGTTTGAG GTCATAAAAGGACAGACCATCGAAGTCCGTTGCGAATCGATCAGTGGAACTTTGCCTATTTCTTACCAAC TTTTAAAAACAAGTAAAGTTTTGGAGAATAGTACCAAGAACTCAAATGATCCTGCGGTATTCAAAGACAA CCCCACTGAAGACGTCGAATACCAGTGTGTTGCAGATAATTGCCATTCCCACGCCAAAATGTTAAGTGAG GTTCTGAGGGTGAAGGTGATAGCCCCGGTGGATGAGGTCCAGATTTCTATCCTGTCAAGTAAGGTGGTGG AGTCTGGAGAGGACATTGTGCTGCAATGTGCTGTGAATGAAGGATCTGGTCCCATCACCTATAAGTTTTA CAGAGAAAAAGAGGGCAAACCCTTCTATCAAATGACCTCAAATGCCACCCAGGCATTTTGGACCAAGCAG AAGGCTAACAAGGAACAGGAGGGAGAGTATTACTGCACAGCCTTCAACAGAGCCAACCACGCCTCCAGTG TCCCCAGAAGCAAAATACTGACAGTCAGAGTCATTCTTGCCCCATGGAAGAAAGGACTTATTGCAGTGGT TATCATCGGAGTGATCATTGCTCTCTTGATCATTGCGGCCAAATGTTATTTTCTGAGGAAAGCCAAGGCC AAGCAGATGCCAGTGGAAATGTCCAGGCCAGCAGTACCACTTCTGAACTCCAACAACGAGAAAATGTCAG ATCCCAATATGGAAGCTAACAGTCATTACGGTCACAATGACGATGTCGGAAACCATGCAATGAAACCAAT AAATGATAATAAAGAGCCTCTGAACTCAGACGTGCAGTACACGGAAGTTCAAGTGTCCTCAGCTGAGTCT CACAAAGATCTAGGAAAGAAGGACACAGAGACAGTGTACAGTGAAGTCCGGAAAGCTGTCCCTGATGCCG TGGAAAGCAGATACTCTAGAACGGAAGGCTCCCTTGATGGAACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208654 representing NM_000442
Red=Cloning site Green=Tags(s) MQPRWAQGATMWLGVLLTLLLCSSLEGQENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQ HQMLFYKDDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQVLVEGVPSPRVTLDKK EAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARI ISGIHMQTSESTKSELVTVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKAIVA HNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSKPELESSFTHLDQGERLNLSCSIP GAPPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFE VIKGQTIEVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSE VLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQ KANKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKGLIAVVIIGVIIALLIIAAKCYFLRKAKA KQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVGNHAMKPINDNKEPLNSDVQYTEVQVSSAES HKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000442 |
| ORF Size | 2214 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000442.2, NP_000433.2 |
| RefSeq Size | 3754 bp |
| RefSeq ORF | 2217 bp |
| Locus ID | 5175 |
| UniProt ID | P16284 |
| Domains | ig, IGc2, IG |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs), Leukocyte transendothelial migration |
| MW | 82.4 kDa |
| Gene Summary | The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208654L1 | Lenti ORF clone of Human platelet/endothelial cell adhesion molecule (PECAM1), Myc-DDK-tagged |
CNY 10,504.00 |
|
| RC208654L2 | Lenti ORF clone of Human platelet/endothelial cell adhesion molecule (PECAM1), mGFP tagged |
CNY 7,700.00 |
|
| RC208654L3 | Lenti ORF clone of Human platelet/endothelial cell adhesion molecule (PECAM1), Myc-DDK-tagged |
CNY 10,504.00 |
|
| RC208654L4 | Lenti ORF clone of Human platelet/endothelial cell adhesion molecule (PECAM1), mGFP tagged |
CNY 10,504.00 |
|
| RG208654 | PECAM1 (tGFP-tagged) - Human platelet/endothelial cell adhesion molecule (PECAM1) |
CNY 9,704.00 |
|
| SC119894 | PECAM1 (untagged)-Human platelet/endothelial cell adhesion molecule (PECAM1) |
CNY 8,120.00 |
