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DLC1 (NM_182643) Human Tagged ORF Clone
CAT＃: RC208691
DLC1 (Myc-DDK-tagged)-Human deleted in liver cancer 1 (DLC1), transcript variant 1
ORF Plasmid: tGFP
"NM_182643" in other vectors (6)
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CNY 10,456.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARHGAP7; HP; p122-RhoGAP; STARD12 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208691 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGTAGCTATCAGAAAGAGAAGCTGGGAAGAACATGTGACCCACTGGATGGGACAGCCTTTTAATT CTGATGATCGTAACACAGCATGTCATCATGGACTAGTAGCTGACAGCTTGCAGGCAAGTATGGAAAAAGA TGCAACTCTAAATGTGGACCGCAAAGAGAAGTGTGTTTCACTACCTGACTGCTGTCATGGATCAGAGCTG AGAGATTTTCCTGGGAGGCCAATGGGTCATCTTTCAAAGGATGTGGACGAAAATGACAGCCATGAAGGTG AAGATCAGTTTCTTTCTCTGGAAGCCAGCACAGAAACACTAGTGCATGTTTCTGATGAGGATAACAATGC TGATTTATGCCTTACAGATGATAAACAGGTTTTAAATACCCAAGGGCAGAAAACATCAGGCCAACATATG ATCCAAGGAGCAGGCTCCTTAGAAAAGGCACTGCCCATCATACAAAGTAACCAAGTTTCTTCTAACTCCT GGGGAATAGCTGGTGAAACTGAATTAGCACTGGTAAAAGAAAGTGGGGAGAGAAAAGTTACTGACTCTAT AAGTAAAAGCCTGGAGCTTTGCAATGAAATAAGCTTAAGTGAAATAAAAGATGCACCCAAAGTAAATGCA GTGGATACTTTGAACGTGAAAGATATTGCACCTGAGAAACAATTGCTTAACTCTGCTGTAATTGCTCAGC AACGAAGGAAACCTGACCCCCCTAAAGATGAAAATGAAAGAAGCACCTGCAATGTAGTACAAGATGAGTT CTTGGATACTCCTTGCACAAACAGAGGACTGCCATTATTAAAAACAGATTTTGGAAGCTGCCTTCTGCAG CCTCCTTCCTGCCCCAATGGAATGTCAGCTGAAAATGGCCTGGAGAAGAGTGGTTTTTCACAACATCAAA ACAAAAGTCCACCAAAGGTCAAGGCAGAAGATGGCATGCAGTGTTTACAATTAAAGGAGACCCTGGCCAC CCAGGAACCCACAGATAACCAAGTCAGACTTCGTAAGAGAAAGGAAATAAGAGAAGATCGAGATAGGGCG CGGCTGGACTCCATGGTGCTGCTGATTATGAAACTGGACCAGCTTGATCAGGACATAGAAAATGCCCTCA GCACCAGCTCCTCTCCATCAGGCACACCAACAAACCTGCGGCGGCACGTTCCTGATCTGGAATCAGGATC TGAAAGTGGAGCAGATACCATTTCAGTAAATCAGACACGAGTAAATTTGTCTTCTGACACTGAGTCCACG GACCTCCCATCTTCCACTCCAGTAGCCAATTCTGGAACCAAACCCAAGACTACGGCTATTCAAGGTATTT CAGAGAAGGAAAAGGCTGAAATTGAAGCCAAGGAAGCTTGTGATTGGCTACGGGCAACTGGTTTCCCCCA GTATGCACAGCTTTATGAAGATTTCCTGTTCCCCATCGATATTTCCTTGGTCAAGAGAGAGCATGATTTT TTGGACAGAGATGCCATTGAGGCTCTATGCAGGCGTCTAAATACTTTAAACAAATGTGCGGTGATGAAGC TAGAAATTAGTCCTCATCGGAAACGAAGTGACGATTCAGACGAGGATGAGCCTTGTGCCATCAGTGGCAA ATGGACTTTCCAAAGGGACAGCAAGAGGTGGTCCCGGCTTGAAGAGTTTGATGTCTTTTCTCCAAAACAA GACCTGGTCCCTGGGTCCCCAGACGACTCCCACCCGAAGGACGGCCCCAGCCCCGGAGGCACGCTGATGG ACCTCAGCGAGCGCCAGGAGGTGTCTTCCGTCCGCAGCCTCAGCAGCACTGGCAGCCTCCCCAGCCACGC TCCCCCCAGCGAGGATGCTGCCACCCCCCGGACTAACTCCGTCATCAGCGTTTGCTCCTCCAGCAACTTG GCAGGCAATGACGACTCTTTCGGCAGCCTGCCCTCTCCCAAGGAACTGTCCAGCTTCAGCTTCAGCATGA AAGGCCACGAAAAAACTGCCAAGTCCAAGACGCGCAGTCTGCTGAAACGGATGGAGAGCCTGAAGCTCAA GAGCTCCCATCACAGCAAGCACAAAGCGCCCTCAAAGCTGGGGTTGATCATCAGCGGGCCCATCTTGCAA GAGGGGATGGATGAGGAGAAGCTGAAGCAGCTCAACTGCGTGGAGATCTCCGCCCTCAATGGCAACCGCA TCAACGTCCCCATGGTACGAAAGAGGAGCGTTTCCAACTCCACGCAGACCAGCAGCAGCAGCAGCCAGTC GGAGACCAGCAGCGCGGTCAGCACGCCCAGCCCTGTTACGAGGACCCGGAGCCTCAGTGCGTGCAACAAG CGGGTGGGCATGTACTTAGAGGGCTTCGATCCTTTCAATCAGTCAACATTTAACAACGTGGTGGAGCAGA ACTTTAAGAACCGCGAGAGCTACCCGGAGGACACGGTGTTCTACATCCCTGAAGATCACAAGCCTGGCAC TTTCCCCAAAGCTCTCACCAATGGCAGTTTCTCCCCCTCGGGGAATAACGGCTCTGTGAACTGGAGGACG GGAAGCTTCCACGGCCCTGGCCACATCAGCCTCAGGAGGGAAAACAGTAGCGACAGCCCCAAGGAACTGA AGAGACGCAATTCTTCCAGCTCCATGAGCAGCCGCCTGAGCATCTACGACAACGTGCCGGGCTCCATCCT CTACTCCAGTTCAGGGGACCTGGCGGATCTGGAGAACGAGGACATCTTCCCCGAGCTGGACGACATCCTC TACCACGTGAAGGGGATGCAGCGGATAGTCAATCAGTGGTCGGAGAAGTTTTCTGATGAGGGAGATTCGG ACTCAGCCCTGGACTCGGTCTCTCCCTGCCCGTCCTCTCCAAAACAGATACACCTGGATGTGGACAACGA CCGAACCACACCCAGCGACCTGGACAGCACAGGCAACTCCCTGAATGAACCGGAAGAGCCCTCCGAGATC CCGGAAAGAAGGGATTCTGGGGTTGGGGCTTCCCTAACCAGGTCCAACAGGCACCGACTGAGATGGCACA GTTTCCAGAGCTCACATCGGCCAAGCCTCAACTCTGTATCACTACAGATTAACTGCCAGTCTGTGGCCCA GATGAACCTGCTGCAGAAATACTCACTCCTAAAGCTAACGGCCCTGCTGGAGAAATACACACCTTCTAAC AAGCATGGTTTTAGCTGGGCCGTGCCCAAGTTCATGAAGAGGATCAAGGTTCCAGACTACAAGGACCGGA GTGTGTTTGGGGTCCCACTGACGGTCAACGTGCAGCGCACAGGACAACCGTTGCCTCAGAGCATCCAGCA GGCCATGCGATACCTCCGGAACCATTGTTTGGATCAGGTTGGGCTCTTCAGAAAATCGGGGGTCAAGTCC CGGATTCAGGCTCTGCGCCAGATGAATGAAGGTGCCATAGACTGTGTCAACTACGAAGGACAGTCTGCTT ATGACGTGGCAGACATGCTGAAGCAGTATTTTCGAGATCTTCCTGAGCCACTAATGACGAACAAACTCTC GGAAACCTTTCTACAGATCTACCAATATGTGCCCAAGGACCAGCGCCTGCAGGCCATCAAGGCTGCCATC ATGCTGCTGCCTGACGAGAACCGGGAGGTTCTGCAGACCCTGCTTTATTTCCTGAGCGATGTCACAGCAG CCGTAAAAGAAAACCAGATGACCCCAACCAACCTGGCCGTGTGCTTAGCGCCTTCCCTCTTCCATCTCAA CACCCTGAAGAGAGAGAATTCCTCTCCCAGGGTAATGCAAAGAAAACAAAGTTTGGGCAAACCAGATCAG AAAGATTTGAATGAAAACCTAGCTGCCACTCAAGGGCTGGCCCATATGATCGCCGAGTGCAAGAAGCTTT TCCAGGTTCCCGAGGAAATGAGCCGATGTCGTAATTCCTATACCGAACAAGAGCTGAAGCCCCTCACTCT GGAAGCACTCGGGCACCTGGGTAATGATGACTCAGCTGACTACCAACACTTCCTCCAGGACTGTGTGGAT GGCCTGTTTAAAGAAGTCAAAGAGAAGTTTAAAGGCTGGGTCAGCTACTCCACTTCGGAGCAGGCTGAGC TGTCCTATAAGAAGGTGAGCGAAGGACCCCCTCTGAGGCTTTGGAGGTCAGTCATTGAAGTCCCTGCTGT GCCAGAGGAAATCTTAAAGCGCCTACTTAAAGAACAGCACCTCTGGGATGTAGACCTGTTGGATTCAAAA GTGATCGAAATTCTGGACAGCCAAACTGAAATTTACCAGTATGTCCAAAACAGTATGGCACCTCATCCTG CTCGAGACTACGTTGTTTTAAGAACCTGGAGGACTAATTTACCCAAAGGAGCCTGTGCCCTTTTACTAAC CTCTGTGGATCACGATCGCGCACCTGTGGTGGGTGTGAGGGTTAATGTGCTCTTGTCCAGGTATTTGATT GAACCCTGTGGGCCAGGAAAATCCAAACTCACCTACATGTGCAGAGTTGACTTAAGGGGCCACATGCCAG AATGGTACACAAAATCTTTTGGACATTTGTGTGCAGCTGAAGTTGTAAAGATCCGGGATTCCTTCAGTAA CCAGAACACTGAAACCAAAGACACCAAATCTAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208691 protein sequence
Red=Cloning site Green=Tags(s) MSVAIRKRSWEEHVTHWMGQPFNSDDRNTACHHGLVADSLQASMEKDATLNVDRKEKCVSLPDCCHGSEL RDFPGRPMGHLSKDVDENDSHEGEDQFLSLEASTETLVHVSDEDNNADLCLTDDKQVLNTQGQKTSGQHM IQGAGSLEKALPIIQSNQVSSNSWGIAGETELALVKESGERKVTDSISKSLELCNEISLSEIKDAPKVNA VDTLNVKDIAPEKQLLNSAVIAQQRRKPDPPKDENERSTCNVVQDEFLDTPCTNRGLPLLKTDFGSCLLQ PPSCPNGMSAENGLEKSGFSQHQNKSPPKVKAEDGMQCLQLKETLATQEPTDNQVRLRKRKEIREDRDRA RLDSMVLLIMKLDQLDQDIENALSTSSSPSGTPTNLRRHVPDLESGSESGADTISVNQTRVNLSSDTEST DLPSSTPVANSGTKPKTTAIQGISEKEKAEIEAKEACDWLRATGFPQYAQLYEDFLFPIDISLVKREHDF LDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSDDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQ DLVPGSPDDSHPKDGPSPGGTLMDLSERQEVSSVRSLSSTGSLPSHAPPSEDAATPRTNSVISVCSSSNL AGNDDSFGSLPSPKELSSFSFSMKGHEKTAKSKTRSLLKRMESLKLKSSHHSKHKAPSKLGLIISGPILQ EGMDEEKLKQLNCVEISALNGNRINVPMVRKRSVSNSTQTSSSSSQSETSSAVSTPSPVTRTRSLSACNK RVGMYLEGFDPFNQSTFNNVVEQNFKNRESYPEDTVFYIPEDHKPGTFPKALTNGSFSPSGNNGSVNWRT GSFHGPGHISLRRENSSDSPKELKRRNSSSSMSSRLSIYDNVPGSILYSSSGDLADLENEDIFPELDDIL YHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDNDRTTPSDLDSTGNSLNEPEEPSEI PERRDSGVGASLTRSNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSN KHGFSWAVPKFMKRIKVPDYKDRSVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKS RIQALRQMNEGAIDCVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAI MLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQ KDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGHLGNDDSADYQHFLQDCVD GLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRSVIEVPAVPEEILKRLLKEQHLWDVDLLDSK VIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLSRYLI EPCGPGKSKLTYMCRVDLRGHMPEWYTKSFGHLCAAEVVKIRDSFSNQNTETKDTKSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_182643 |
| ORF Size | 4584 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_182643.3 |
| RefSeq Size | 7479 bp |
| RefSeq ORF | 4587 bp |
| Locus ID | 10395 |
| UniProt ID | Q96QB1 |
| MW | 170.6 kDa |
| Gene Summary | This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.[provided by RefSeq, Apr 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208691L1 | Lenti ORF clone of Human deleted in liver cancer 1 (DLC1), transcript variant 1, Myc-DDK-tagged |
CNY 12,856.00 |
|
| RC208691L2 | Lenti ORF clone of Human deleted in liver cancer 1 (DLC1), transcript variant 1, mGFP tagged |
CNY 17,100.00 |
|
| RC208691L3 | Lenti ORF clone of Human deleted in liver cancer 1 (DLC1), transcript variant 1, Myc-DDK-tagged |
CNY 17,100.00 |
|
| RC208691L4 | Lenti ORF clone of Human deleted in liver cancer 1 (DLC1), transcript variant 1, mGFP tagged |
CNY 17,100.00 |
|
| RG208691 | DLC1 (tGFP-tagged) - Human deleted in liver cancer 1 (DLC1), transcript variant 1 |
CNY 16,720.00 |
|
| SC307473 | DLC1 (untagged)-Human deleted in liver cancer 1 (DLC1), transcript variant 1 |
CNY 10,450.00 |
