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DOC2A (NM_003586) Human Tagged ORF Clone
CAT＃: RC208788
- TrueORF®
DOC2A (Myc-DDK-tagged)-Human double C2-like domains, alpha (DOC2A)
ORF Plasmid: tGFP
"NM_003586" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Doc2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208788 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGAGGGGCCGCAGGGGCGATCGCATGACCATCAACATCCAGGAGCACATGGCCATCAACGTGTGCCCCG GGCCCATCCGGCCCATCCGCCAGATCTCTGACTACTTCCCCCGGGGACCAGGACCTGAAGGGGGCGGCGG GGGCGGCGGGGAGGCCCCCGCCCATCTGGTCCCCCTGGCTCTGGCCCCCCCTGCAGCCCTCCTTGGGGCC ACCACGCCTGAGGATGGTGCGGAGGTGGACAGCTATGACTCGGATGATGCCACCGCCCTAGGCACGCTGG AGTTTGACCTTCTCTACGACCGGGCCTCCTGCACTCTGCACTGTAGCATCCTCAGGGCCAAGGGCCTCAA GCCCATGGATTTCAATGGCCTCGCCGACCCCTACGTCAAGCTGCACTTGCTGCCTGGAGCCTGTAAGGCC AATAAGCTAAAAACGAAGACTCAGAGGAACACACTGAATCCCGTGTGGAATGAGGACCTGACTTACAGCG GGATCACAGATGACGACATCACGCACAAGGTGCTCAGGATCGCCGTCTGTGATGAGGACAAGCTGAGTCA CAATGAGTTTATTGGGGAGATCCGCGTGCCCCTCCGCCGCCTCAAGCCTTCGCAGAAGAAGCATTTTAAC ATCTGCCTCGAGCGCCAGGTCCCGCTGGCGTCCCCCTCTTCCATGTCAGCGGCGCTGAGGGGCATCTCCT GTTATCTGAAGGAGTTGGAGCAGGCGGAGCAGGGGCAGGGGCTGCTGGAGGAGCGTGGCCGCATCCTGCT GAGTCTCAGCTACAGCTCGCGGCGCCGGGGACTGCTGGTAGGCATCTTGCGCTGCGCCCATCTGGCTGCC ATGGACGTCAACGGTTACTCGGACCCCTACGTCAAGACGTACCTGAGGCCCGATGTGGACAAGAAATCCA AGCATAAGACGTGTGTGAAGAAGAAGACTCTCAACCCAGAATTTAACGAGGAGTTTTTCTACGAGATAGA GCTCTCCACTCTGGCCACCAAGACCCTGGAAGTCACCGTCTGGGACTATGACATTGGCAAATCCAATGAC TTCATTGGTGGCGTGTCCCTGGGGCCAGGTGCCCGAGGCGAGGCTCGGAAGCACTGGAGTGACTGCCTGC AGCAGCCGGACGCAGCCCTGGAGCGCTGGCACACCCTGACCAGTGAGCTGCCCCCTGCGGCCGGGGCTCT GTCCTCAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208788 protein sequence
Red=Cloning site Green=Tags(s) MRGRRGDRMTINIQEHMAINVCPGPIRPIRQISDYFPRGPGPEGGGGGGGEAPAHLVPLALAPPAALLGA TTPEDGAEVDSYDSDDATALGTLEFDLLYDRASCTLHCSILRAKGLKPMDFNGLADPYVKLHLLPGACKA NKLKTKTQRNTLNPVWNEDLTYSGITDDDITHKVLRIAVCDEDKLSHNEFIGEIRVPLRRLKPSQKKHFN ICLERQVPLASPSSMSAALRGISCYLKELEQAEQGQGLLEERGRILLSLSYSSRRRGLLVGILRCAHLAA MDVNGYSDPYVKTYLRPDVDKKSKHKTCVKKKTLNPEFNEEFFYEIELSTLATKTLEVTVWDYDIGKSND FIGGVSLGPGARGEARKHWSDCLQQPDAALERWHTLTSELPPAAGALSSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003586 |
| ORF Size | 1200 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003586.3 |
| RefSeq Size | 2089 bp |
| RefSeq ORF | 1203 bp |
| Locus ID | 8448 |
| UniProt ID | Q14183 |
| Protein Families | Secreted Protein |
| MW | 44 kDa |
| Gene Summary | There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208788L3 | Lenti-ORF clone of DOC2A (Myc-DDK-tagged)-Human double C2-like domains, alpha (DOC2A) |
CNY 5,890.00 |
|
| RC208788L4 | Lenti-ORF clone of DOC2A (mGFP-tagged)-Human double C2-like domains, alpha (DOC2A) |
CNY 5,890.00 |
|
| RG208788 | DOC2A (tGFP-tagged) - Human double C2-like domains, alpha (DOC2A) |
CNY 4,370.00 |
|
| SC126140 | DOC2A (untagged)-Human double C2-like domains, alpha (DOC2A) |
CNY 3,656.00 |
