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GRAP (NM_006613) Human Tagged ORF Clone
CAT＃: RC208908
GRAP (Myc-DDK-tagged)-Human GRB2-related adaptor protein (GRAP)
ORF Plasmid: tGFP
"NM_006613" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DFNB114 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208908 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCCGTGGCCCTGTACAGCTTTCAGGCTACAGAGAGCGACGAGCTGGCCTTCAACAAGGGAGACA CACTCAAGATCCTGAACATGGAGGATGACCAGAACTGGTACAAGGCCGAGCTCCGGGGTGTCGAGGGATT TATTCCCAAGAACTACATCCGCGTCAAGCCCCATCCGTGGTACTCGGGCAGGATTTCCCGGCAGCTGGCC GAAGAGATTCTGATGAAGCGGAACCATCTGGGAGCCTTCCTGATCCGGGAGAGTGAGAGCTCCCCAGGGG AGTTCTCTGTGTCTGTGAACTATGGAGACCAGGTGCAGCACTTCAAGGTGCTGCGTGAGGCCTCGGGGAA GTACTTCCTGTGGGAGGAGAAGTTCAACTCCCTCAACGAGCTGGTCGACTTCTACCGCACCACCACCATC GCCAAGAAGCGGCAGATCTTCCTGCGCGACGAGGAGCCCTTGCTCAAGTCACCTGGGGCCTGCTTTGCCC AGGCCCAGTTTGACTTCTCAGCCCAGGACCCCTCGCAGCTCAGCTTCCGCCGTGGCGACATCATTGAGGT CCTGGAGCGCCCAGACCCCCACTGGTGGCGGGGCCGGTCCTGCGGGCGCGTTGGCTTCTTCCCACGGAGT TACGTGCAGCCCGTGCACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208908 protein sequence
Red=Cloning site Green=Tags(s) MESVALYSFQATESDELAFNKGDTLKILNMEDDQNWYKAELRGVEGFIPKNYIRVKPHPWYSGRISRQLA EEILMKRNHLGAFLIRESESSPGEFSVSVNYGDQVQHFKVLREASGKYFLWEEKFNSLNELVDFYRTTTI AKKRQIFLRDEEPLLKSPGACFAQAQFDFSAQDPSQLSFRRGDIIEVLERPDPHWWRGRSCGRVGFFPRS YVQPVHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006613 |
| ORF Size | 651 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006613.4 |
| RefSeq Size | 2089 bp |
| RefSeq ORF | 654 bp |
| Locus ID | 10750 |
| UniProt ID | Q13588 |
| MW | 25.3 kDa |
| Gene Summary | This gene encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway. [provided by RefSeq, Jul 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208908L1 | Lenti ORF clone of Human GRB2-related adaptor protein (GRAP), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC208908L2 | Lenti ORF clone of Human GRB2-related adaptor protein (GRAP), mGFP tagged |
CNY 5,890.00 |
|
| RC208908L3 | Lenti ORF clone of Human GRB2-related adaptor protein (GRAP), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208908L4 | Lenti ORF clone of Human GRB2-related adaptor protein (GRAP), mGFP tagged |
CNY 5,890.00 |
|
| RG208908 | GRAP (tGFP-tagged) - Human GRB2-related adaptor protein (GRAP) |
CNY 4,000.00 |
|
| SC126093 | GRAP (untagged)-Human GRB2-related adaptor protein (GRAP) |
CNY 2,400.00 |
