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Phosphoserine phosphatase (PSPH) (NM_004577) Human Tagged ORF Clone
CAT＃: RC209090
PSPH (Myc-DDK-tagged)-Human phosphoserine phosphatase (PSPH)
ORF Plasmid: tGFP
"NM_004577" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PSP; PSPHD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209090 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTCCCACTCAGAGCTGAGGAAGCTTTTCTACTCAGCAGATGCTGTGTGTTTTGATGTTGACAGCA CGGTCATCAGAGAAGAAGGAATCGATGAGCTAGCCAAAATCTGTGGCGTTGAGGACGCGGTGTCAGAAAT GACACGGCGAGCCATGGGCGGGGCAGTGCCTTTCAAAGCTGCTCTCACAGAGCGCTTAGCCCTCATCCAG CCCTCCAGGGAGCAGGTGCAGAGACTCATAGCAGAGCAACCCCCACACCTGACCCCCGGCATAAGGGAGC TGGTAAGTCGCCTACAGGAGCGAAATGTTCAGGTTTTCCTAATATCTGGTGGCTTTAGGAGTATTGTAGA GCATGTTGCTTCAAAGCTCAATATCCCAGCAACCAATGTATTTGCCAATAGGCTGAAATTCTACTTTAAC GGTGAATATGCAGGTTTTGATGAGACGCAGCCAACAGCTGAATCTGGTGGAAAAGGAAAAGTGATTAAAC TTTTAAAGGAAAAATTTCATTTTAAGAAAATAATCATGATTGGAGATGGTGCCACAGATATGGAAGCCTG TCCTCCTGCTGATGCTTTCATTGGATTTGGAGGAAATGTGATCAGGCAACAAGTCAAGGATAACGCCAAA TGGTATATCACTGATTTTGTAGAGCTGCTGGGAGAACTGGAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209090 protein sequence
Red=Cloning site Green=Tags(s) MVSHSELRKLFYSADAVCFDVDSTVIREEGIDELAKICGVEDAVSEMTRRAMGGAVPFKAALTERLALIQ PSREQVQRLIAEQPPHLTPGIRELVSRLQERNVQVFLISGGFRSIVEHVASKLNIPATNVFANRLKFYFN GEYAGFDETQPTAESGGKGKVIKLLKEKFHFKKIIMIGDGATDMEACPPADAFIGFGGNVIRQQVKDNAK WYITDFVELLGELEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004577 |
| ORF Size | 675 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004577.4 |
| RefSeq Size | 2142 bp |
| RefSeq ORF | 678 bp |
| Locus ID | 5723 |
| UniProt ID | P78330 |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Glycine, serine and threonine metabolism, Metabolic pathways |
| MW | 25 kDa |
| Gene Summary | The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209090L1 | Lenti ORF clone of Human phosphoserine phosphatase (PSPH), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209090L2 | Lenti ORF clone of Human phosphoserine phosphatase (PSPH), mGFP tagged |
CNY 5,890.00 |
|
| RC209090L3 | Lenti ORF clone of Human phosphoserine phosphatase (PSPH), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209090L4 | Lenti ORF clone of Human phosphoserine phosphatase (PSPH), mGFP tagged |
CNY 4,800.00 |
|
| RG209090 | PSPH (tGFP-tagged) - Human phosphoserine phosphatase (PSPH) |
CNY 4,000.00 |
|
| SC128000 | PSPH (untagged)-Human phosphoserine phosphatase (PSPH) |
CNY 2,400.00 |
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