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eNOS (NOS3) (NM_000603) Human Tagged ORF Clone
CAT＃: RC209228
NOS3 (Myc-DDK-tagged)-Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1
ORF Plasmid: tGFP
"NM_000603" in other vectors (6)
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CNY 12,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ECNOS; eNOS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209228 representing NM_000603.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGCAACTTGAAGAGCGTGGCCCAGGAGCCTGGGCCACCCTGCGGCCTGGGGCTGGGGCTGGGCCTT GGGCTGTGCGGCAAGCAGGGCCCAGCCACCCCGGCCCCTGAGCCCAGCCGGGCCCCAGCATCCCTACTC CCACCAGCGCCAGAACACAGCCCCCCGAGCTCCCCGCTAACCCAGCCCCCAGAGGGGCCCAAGTTCCCT CGTGTGAAGAACTGGGAGGTGGGGAGCATCACCTATGACACCCTCAGCGCCCAGGCGCAGCAGGATGGG CCCTGCACCCCAAGACGCTGCCTGGGCTCCCTGGTATTTCCACGGAAACTACAGGGCCGGCCCTCCCCC GGCCCCCCGGCCCCTGAGCAGCTGCTGAGTCAGGCCCGGGACTTCATCAACCAGTACTACAGCTCCATT AAGAGGAGCGGCTCCCAGGCCCACGAACAGCGGCTTCAAGAGGTGGAAGCCGAGGTGGCAGCCACAGGC ACCTACCAGCTTAGGGAGAGCGAGCTGGTGTTCGGGGCTAAGCAGGCCTGGCGCAACGCTCCCCGCTGC GTGGGCCGGATCCAGTGGGGGAAGCTGCAGGTGTTCGATGCCCGGGACTGCAGGTCTGCACAGGAAATG TTCACCTACATCTGCAACCACATCAAGTATGCCACCAACCGGGGCAACCTTCGCTCGGCCATCACAGTG TTCCCGCAGCGCTGCCCTGGCCGAGGAGACTTCCGAATCTGGAACAGCCAGCTGGTGCGCTACGCGGGC TACCGGCAGCAGGACGGCTCTGTGCGGGGGGACCCAGCCAACGTGGAGATCACCGAGCTCTGCATTCAG CACGGCTGGACCCCAGGAAACGGTCGCTTCGACGTGCTGCCCCTGCTGCTGCAGGCCCCAGATGAGCCC CCAGAACTCTTCCTTCTGCCCCCCGAGCTGGTCCTTGAGGTGCCCCTGGAGCACCCCACGCTGGAGTGG TTTGCAGCCCTGGGCCTGCGCTGGTACGCCCTCCCGGCAGTGTCCAACATGCTGCTGGAAATTGGGGGC CTGGAGTTCCCCGCAGCCCCCTTCAGTGGCTGGTACATGAGCACTGAGATCGGCACGAGGAACCTGTGT GACCCTCACCGCTACAACATCCTGGAGGATGTGGCTGTCTGCATGGACCTGGATACCCGGACCACCTCG TCCCTGTGGAAAGACAAGGCAGCAGTGGAAATCAACGTGGCCGTGCTGCACAGTTACCAGCTAGCCAAA GTCACCATCGTGGACCACCACGCCGCCACGGCCTCTTTCATGAAGCACCTGGAGAATGAGCAGAAGGCC AGGGGGGGCTGCCCTGCAGACTGGGCCTGGATCGTGCCCCCCATCTCGGGCAGCCTCACTCCTGTTTTC CATCAGGAGATGGTCAACTATTTCCTGTCCCCGGCCTTCCGCTACCAGCCAGACCCCTGGAAGGGGAGT GCCGCCAAGGGCACCGGCATCACCAGGAAGAAGACCTTTAAAGAAGTGGCCAACGCCGTGAAGATCTCC GCCTCGCTCATGGGCACGGTGATGGCGAAGCGAGTGAAGGCGACAATCCTGTATGGCTCCGAGACCGGC CGGGCCCAGAGCTACGCACAGCAGCTGGGGAGACTCTTCCGGAAGGCTTTTGATCCCCGGGTCCTGTGT ATGGATGAGTATGACGTGGTGTCCCTCGAACACGAGACGCTGGTGCTGGTGGTAACCAGCACATTTGGG AATGGGGATCCCCCGGAGAATGGAGAGAGCTTTGCAGCTGCCCTGATGGAGATGTCCGGCCCCTACAAC AGCTCCCCTCGGCCGGAACAGCACAAGAGTTATAAGATCCGCTTCAACAGCATCTCCTGCTCAGACCCA CTGGTGTCCTCTTGGCGGCGGAAGAGGAAGGAGTCCAGTAACACAGACAGTGCAGGGGCCCTGGGCACC CTCAGGTTCTGTGTGTTCGGGCTCGGCTCCCGGGCATACCCCCACTTCTGCGCCTTTGCTCGTGCGGTG GACACACGGCTGGAGGAACTGGGCGGGGAGCGGCTGCTGCAGCTGGGCCAGGGCGACGAGCTGTGCGGC CAGGAGGAGGCCTTCCGAGGCTGGGCCCAGGCTGCCTTCCAGGCCGCCTGTGAGACCTTCTGTGTGGGA GAGGATGCCAAGGCCGCCGCCCGAGACATCTTCAGCCCCAAACGGAGCTGGAAGCGCCAGAGGTACCGG CTGAGCGCCCAGGCCGAGGGCCTGCAGTTGCTGCCAGGTCTGATCCACGTGCACAGGCGGAAGATGTTC CAGGCTACAATCCGCTCAGTGGAAAACCTGCAAAGCAGCAAGTCCACGAGGGCCACCATCCTGGTGCGC CTGGACACCGGAGGCCAGGAGGGGCTGCAGTACCAGCCGGGGGACCACATAGGTGTCTGCCCGCCCAAC CGGCCCGGCCTTGTGGAGGCGCTGCTGAGCCGCGTGGAGGACCCGCCGGCGCCCACTGAGCCCGTGGCA GTAGAGCAGCTGGAGAAGGGCAGCCCTGGTGGCCCTCCCCCCGGCTGGGTGCGGGACCCCCGGCTGCCC CCGTGCACGCTGCGCCAGGCTCTCACCTTCTTCCTGGACATCACCTCCCCACCCAGCCCTCAGCTCTTG CGGCTGCTCAGCACCTTGGCAGAAGAGCCCAGGGAACAGCAGGAGCTGGAGGCCCTCAGCCAGGATCCC CGACGCTACGAGGAGTGGAAGTGGTTCCGCTGCCCCACGCTGCTGGAGGTGCTGGAGCAGTTCCCGTCG GTGGCGCTGCCTGCCCCACTGCTCCTCACCCAGCTGCCTCTGCTCCAGCCCCGGTACTACTCAGTCAGC TCGGCACCCAGCACCCACCCAGGAGAGATCCACCTCACTGTAGCTGTGCTGGCATACAGGACTCAGGAT GGGCTGGGCCCCCTGCACTATGGAGTCTGCTCCACGTGGCTAAGCCAGCTCAAGCCCGGAGACCCTGTG CCCTGCTTCATCCGGGGGGCTCCCTCCTTCCGGCTGCCACCCGATCCCAGCTTGCCCTGCATCCTGGTG GGTCCAGGCACTGGCATTGCCCCCTTCCGGGGATTCTGGCAGGAGCGGCTGCATGACATTGAGAGCAAA GGGCTGCAGCCCACTCCCATGACTTTGGTGTTCGGCTGCCGATGCTCCCAACTTGACCATCTCTACCGC GACGAGGTGCAGAACGCCCAGCAGCGCGGGGTGTTTGGCCGAGTCCTCACCGCCTTCTCCCGGGAACCT GACAACCCCAAGACCTACGTGCAGGACATCCTGAGGACGGAGCTGGCTGCGGAGGTGCACCGCGTGCTG TGCCTCGAGCGGGGCCACATGTTTGTCTGCGGCGATGTTACCATGGCAACCAACGTCCTGCAGACCGTG CAGCGCATCCTGGCGACGGAGGGCGACATGGAGCTGGACGAGGCCGGCGACGTCATCGGCGTGCTGCGG GATCAGCAACGCTACCACGAAGACATTTTCGGGCTCACGCTGCGCACCCAGGAGGTGACAAGCCGCATA CGCACCCAGAGCTTTTCCTTGCAGGAGCGTCAGTTGCGGGGCGCAGTGCCCTGGGCGTTCGACCCTCCC GGCTCAGACACCAACAGCCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC209228
Blue=ORF Red=Cloning site Green=Tag(s) MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFP RVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSI KRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEM FTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQ HGWTPGNGRFDVLPLLLQAPDEPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGG LEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAK VTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGS AAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLC MDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDP LVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCG QEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMF QATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVA VEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDP RRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQD GLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESK GLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVL CLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRI RTQSFSLQERQLRGAVPWAFDPPGSDTNSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC209228 also available, TP309228 |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000603 |
| ORF Size | 3609 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq Size | 4345 bp |
| RefSeq ORF | 3612 bp |
| Locus ID | 4846 |
| UniProt ID | P29474 |
| Domains | flavodoxin, NAD_binding_1, FAD_binding_1, NO_synthase |
| Protein Families | Druggable Genome |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway |
| MW | 133.3 kDa |
| Gene Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
C. pneumoniae disrupts eNOS trafficking and impairs NO production in human aortic endothelial cells
,Mueller, KE;Wolf, K;,
Cell. Microbiol.
,PubMed ID 25131610
[eNOS]
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Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209228L1 | Lenti ORF clone of Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1, Myc-DDK-tagged |
CNY 14,960.00 |
|
| RC209228L2 | Lenti ORF clone of Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1, mGFP tagged |
CNY 7,600.00 |
|
| RC209228L3 | Lenti ORF clone of Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1, Myc-DDK-tagged |
CNY 14,960.00 |
|
| RC209228L4 | Lenti ORF clone of Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1, mGFP tagged |
CNY 14,960.00 |
|
| RG209228 | NOS3 (tGFP-tagged) - Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1 |
CNY 14,160.00 |
|
| SC108440 | NOS3 (untagged)-Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1 |
CNY 12,576.00 |
