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VGluT1 (SLC17A7) (NM_020309) Human Tagged ORF Clone
CAT＃: RC209230
SLC17A7 (Myc-DDK-tagged)-Human solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 (SLC17A7)
ORF Plasmid: tGFP
"NM_020309" in other vectors (6)
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CNY 6,256.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BNPI; VGLUT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209230 representing NM_020309
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCCGCCAGGAGGAGTTTCGGAAGCTAGCGGGTCGTGCTCTCGGGAAGCTGCACCGCCTTCTGG AGAAGCGGCAGGAAGGCGCGGAGACGCTGGAGCTGAGTGCGGATGGGCGCCCGGTGACCACGCAGACCCG GGACCCGCCGGTGGTGGACTGCACCTGCTTCGGCCTCCCTCGCCGCTACATTATCGCCATCATGAGTGGT CTGGGCTTCTGCATCAGCTTTGGCATCCGCTGCAACCTGGGCGTGGCCATCGTCTCCATGGTCAATAACA GCACGACCCACCGCGGGGGCCACGTGGTGGTGCAGAAAGCCCAGTTCAGCTGGGATCCAGAGACTGTCGG CCTCATACACGGCTCCTTTTTCTGGGGCTACATTGTCACTCAGATTCCAGGAGGATTTATCTGTCAAAAA TTTGCAGCCAACAGAGTTTTCGGCTTTGCTATTGTGGCAACATCCACTCTAAACATGCTGATCCCCTCAG CTGCCCGCGTCCACTATGGCTGTGTCATCTTCGTGAGGATCCTGCAGGGGTTGGTAGAGGGGGTCACATA CCCCGCCTGCCATGGGATCTGGAGCAAATGGGCCCCACCCTTAGAACGGAGTCGCCTGGCGACGACAGCC TTTTGTGGTTCCTATGCTGGGGCGGTGGTCGCGATGCCCCTCGCCGGGGTCCTTGTGCAGTACTCAGGAT GGAGCTCTGTTTTCTACGTCTACGGCAGCTTCGGGATCTTCTGGTACCTGTTCTGGCTGCTCGTCTCCTA CGAGTCCCCCGCGCTGCACCCCAGCATCTCGGAGGAGGAGCGCAAGTACATCGAGGACGCCATCGGAGAG AGCGCGAAACTCATGAACCCCCTCACGAAGTTTAGCACTCCCTGGCGGCGCTTCTTCACGTCTATGCCAG TCTATGCCATCATCGTGGCCAACTTCTGCCGCAGCTGGACGTTCTACCTGCTGCTCATCTCCCAGCCCGC CTACTTCGAAGAAGTGTTCGGCTTCGAGATCAGCAAGGTAGGCCTGGTGTCCGCGCTGCCCCACCTGGTC ATGACCATCATCGTGCCCATCGGCGGCCAGATCGCGGACTTCCTGCGGAGCCGCCGCATCATGTCCACCA CCAACGTGCGCAAGTTGATGAACTGCGGAGGCTTCGGCATGGAAGCCACGCTGCTGTTGGTGGTCGGCTA CTCGCACTCCAAGGGCGTGGCCATCTCCTTCCTGGTCCTAGCCGTGGGCTTCAGCGGCTTCGCCATCTCT GGGTTCAACGTGAACCACCTGGACATAGCCCCGCGCTACGCCAGCATCCTCATGGGCATCTCCAACGGCG TGGGCACACTGTCGGGCATGGTGTGCCCCATCATCGTGGGGGCCATGACTAAGCACAAGACTCGGGAGGA GTGGCAGTACGTGTTCCTAATTGCCTCCCTGGTGCACTATGGAGGTGTCATCTTCTACGGGGTCTTTGCT TCTGGAGAGAAGCAGCCGTGGGCAGAGCCTGAGGAGATGAGCGAGGAGAAGTGTGGCTTCGTTGGCCATG ACCAGCTGGCTGGCAGTGACGACAGCGAAATGGAGGATGAGGCTGAGCCCCCGGGGGCACCCCCTGCACC CCCGCCCTCCTATGGGGCCACACACAGCACATTTCAGCCCCCCAGGCCCCCACCCCCTGTCCGGGACTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209230 representing NM_020309
Red=Cloning site Green=Tags(s) MEFRQEEFRKLAGRALGKLHRLLEKRQEGAETLELSADGRPVTTQTRDPPVVDCTCFGLPRRYIIAIMSG LGFCISFGIRCNLGVAIVSMVNNSTTHRGGHVVVQKAQFSWDPETVGLIHGSFFWGYIVTQIPGGFICQK FAANRVFGFAIVATSTLNMLIPSAARVHYGCVIFVRILQGLVEGVTYPACHGIWSKWAPPLERSRLATTA FCGSYAGAVVAMPLAGVLVQYSGWSSVFYVYGSFGIFWYLFWLLVSYESPALHPSISEEERKYIEDAIGE SAKLMNPLTKFSTPWRRFFTSMPVYAIIVANFCRSWTFYLLLISQPAYFEEVFGFEISKVGLVSALPHLV MTIIVPIGGQIADFLRSRRIMSTTNVRKLMNCGGFGMEATLLLVVGYSHSKGVAISFLVLAVGFSGFAIS GFNVNHLDIAPRYASILMGISNGVGTLSGMVCPIIVGAMTKHKTREEWQYVFLIASLVHYGGVIFYGVFA SGEKQPWAEPEEMSEEKCGFVGHDQLAGSDDSEMEDEAEPPGAPPAPPPSYGATHSTFQPPRPPPPVRDY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020309 |
| ORF Size | 1680 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020309.4 |
| RefSeq Size | 2993 bp |
| RefSeq ORF | 1683 bp |
| Locus ID | 57030 |
| UniProt ID | Q9P2U7 |
| Domains | sugar_tr |
| Protein Families | Transmembrane |
| MW | 61.6 kDa |
| Gene Summary | The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209230L1 | Lenti-ORF clone of SLC17A7 (Myc-DDK-tagged)-Human solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 (SLC17A7) |
CNY 8,656.00 |
|
| RC209230L2 | Lenti-ORF clone of SLC17A7 (mGFP-tagged)-Human solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 (SLC17A7) |
CNY 5,890.00 |
|
| RC209230L3 | Lenti-ORF clone of SLC17A7 (Myc-DDK-tagged)-Human solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 (SLC17A7) |
CNY 5,890.00 |
|
| RC209230L4 | Lenti-ORF clone of SLC17A7 (mGFP-tagged)-Human solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 (SLC17A7) |
CNY 8,656.00 |
|
| RG209230 | SLC17A7 (tGFP-tagged) - Human solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 (SLC17A7) |
CNY 4,370.00 |
|
| SC113161 | SLC17A7 (untagged)-Human solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 (SLC17A7) |
CNY 6,264.00 |
