FPR3 (NM_002030) Human Tagged ORF Clone

CAT#: RC209257

  • TrueORF®

FPR3 (Myc-DDK-tagged)-Human formyl peptide receptor 3 (FPR3)

ORF Plasmid: DDK tGFP



  "NM_002030" in other vectors (6)

Need custom modification / cloning service?
Get a free quote

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-FPR3 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms FML2_HUMAN; FMLP-R-II; FMLPY; FPRH1; FPRH2; FPRL2; RMLP-R-I
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209257 representing NM_002030.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAAACCAACTTCTCCATTCCTCTGAATGAAACTGAGGAGGTGCTCCCTGAGCCTGCTGGCCACACC
GTTCTGTGGATCTTCTCATTGCTAGTCCACGGAGTCACCTTTGTCTTCGGGGTCCTGGGCAATGGGCTT
GTGATCTGGGTGGCTGGATTCCGGATGACACGCACAGTCAACACCATCTGTTACCTGAACCTGGCCCTA
GCTGACTTCTCTTTCAGTGCCATCCTACCATTCCGAATGGTCTCAGTCGCCATGAGAGAAAAATGGCCT
TTTGGCTCATTCCTATGTAAGTTAGTTCATGTTATGATAGACATCAACCTGTTTGTCAGTGTCTACCTG
ATCACCATCATTGCTCTGGACCGCTGTATTTGTGTCCTGCATCCAGCCTGGGCCCAGAACCATCGCACC
ATGAGTCTGGCCAAGAGGGTGATGACGGGACTCTGGATTTTCACCATAGTCCTTACCTTACCAAATTTC
ATCTTCTGGACTACAATAAGTACTACGAATGGGGACACATACTGTATTTTCAACTTTGCATTCTGGGGT
GACACTGCTGTAGAGAGGTTGAACGTGTTCATTACCATGGCCAAGGTCTTTCTGATCCTCCACTTCATT
ATTGGCTTCAGCGTGCCTATGTCCATCATCACAGTCTGCTATGGGATCATCGCTGCCAAAATTCACAGA
AACCACATGATTAAATCCAGCCGTCCCTTACGTGTCTTCGCTGCTGTGGTGGCTTCTTTCTTCATCTGT
TGGTTCCCTTATGAACTAATTGGCATTCTAATGGCAGTCTGGCTCAAAGAGATGTTGTTAAATGGCAAA
TACAAAATCATTCTTGTCCTGATTAACCCAACAAGCTCCTTGGCCTTTTTTAACAGCTGCCTCAACCCA
ATTCTCTACGTCTTTATGGGTCGTAACTTCCAAGAAAGACTGATTCGCTCTTTGCCCACTAGTTTGGAG
AGGGCCCTGACTGAGGTCCCTGACTCAGCCCAGACCAGCAACACAGACACCACTTCTGCTTCACCTCCT
GAGGAGACGGAGTTACAAGCAATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>Peptide sequence encoded by RC209257
Blue=ORF Red=Cloning site Green=Tag(s)

METNFSIPLNETEEVLPEPAGHTVLWIFSLLVHGVTFVFGVLGNGLVIWVAGFRMTRTVNTICYLNLAL
ADFSFSAILPFRMVSVAMREKWPFGSFLCKLVHVMIDINLFVSVYLITIIALDRCICVLHPAWAQNHRT
MSLAKRVMTGLWIFTIVLTLPNFIFWTTISTTNGDTYCIFNFAFWGDTAVERLNVFITMAKVFLILHFI
IGFSVPMSIITVCYGIIAAKIHRNHMIKSSRPLRVFAAVVASFFICWFPYELIGILMAVWLKEMLLNGK
YKIILVLINPTSSLAFFNSCLNPILYVFMGRNFQERLIRSLPTSLERALTEVPDSAQTSNTDTTSASPP
EETELQAM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002030
ORF Size 1059 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002030.5
RefSeq Size 2517 bp
RefSeq ORF 1062 bp
Locus ID 2359
UniProt ID P25089
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 40 kDa
Gene Summary Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...