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PNPLA3 (NM_025225) Human Tagged ORF Clone
CAT＃: RC209577
PNPLA3 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 3 (PNPLA3)
ORF Plasmid: tGFP
"NM_025225" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 4,070.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ADPN; C22orf20; iPLA(2)epsilon |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209577 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGACGCAGAGCGCGGCTGGAGCTTGTCCTTCGCGGGCTGCGGCTTCCTGGGCTTCTACCACGTCG GGGCGACCCGCTGCCTGAGCGAGCACGCCCCGCACCTCCTCCGCGACGCGCGCATGTTGTTCGGCGCTTC GGCCGGGGCGTTGCACTGCGTCGGCGTCCTCTCCGGTATCCCGCTGGAGCAGACTCTGCAGGTCCTCTCA GATCTTGTGCGGAAGGCCAGGAGTCGGAACATTGGCATCTTCCATCCATCCTTCAACTTAAGCAAGTTCC TCCGACAGGGTCTCGGCAAATGCCTCCCGGCCAATGTCCACCAGCTCATCTCCGGCAAAATAGGCATCTC TCTTACCAGAGTGTCTGATGGGGAAAACGTTCTGGTGTCTGACTTTCGGTCCAAAGACGAAGTCGTGGAT GCCTTGGTATGTTCCTGCTTCATGCCTTTCTACAGTGGCCTTATCCCTCCTTCCTTCAGAGGCGTGCGAT ATGTGGATGGAGGAGTGAGTGACAACGTACCCTTCATTGATGCCAAAACAACCATCACCGTGTCCCCCTT CTATGGGGAGTACGACATCTGCCCTAAAGTCAAGTCCACGAACTTTCTTCATGTGGACATCACCAAGCTC AGTCTACGCCTCTGCACAGGGAACCTCTACCTTCTCTCGAGAGCTTTTGTCCCCCCGGATCTCAAGGTGC TGGGAGAGATATGCCTTCGAGGATATTTGGATGCATTCAGGTTCTTGGAAGAGAAGGGCATCTGCAACAG GCCCCAGCCAGGCCTGAAGTCATCCTCAGAAGGGATGGATCCTGAGGTCGCCATGCCCAGCTGGGCAAAC ATGAGTCTGGATTCTTCCCCGGAGTCGGCTGCCTTGGCTGTGAGGCTGGAGGGAGATGAGCTGCTAGACC ACCTGCGTCTCAGCATCCTGCCCTGGGATGAGAGCATCCTGGACACCCTCTCGCCCAGGCTCGCTACAGC ACTGAGTGAAGAAATGAAAGACAAAGGTGGATACATGAGCAAGATTTGCAACTTGCTACCCATTAGGATA ATGTCTTATGTAATGCTGCCCTGTACCCTGCCTGTGGAATCTGCCATTGCGATTGTCCAGAGACTGGTGA CATGGCTTCCAGATATGCCCGACGATGTCCTGTGGTTGCAGTGGGTGACCTCACAGGTGTTCACTCGAGT GCTGATGTGTCTGCTCCCCGCCTCCAGGTCCCAAATGCCAGTGAGCAGCCAACAGGCCTCCCCATGCACA CCTGAGCAGGACTGGCCCTGCTGGACTCCCTGCTCCCCCGAGGGCTGTCCAGCAGAGACCAAAGCAGAGG CCACCCCGCGGTCCATCCTCAGGTCCAGCCTGAACTTCTTCTTGGGCAATAAAGTACCTGCTGGTGCTGA GGGGCTCTCCACCTTTCCCAGTTTTTCACTAGAGAAGAGTCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209577 protein sequence
Red=Cloning site Green=Tags(s) MYDAERGWSLSFAGCGFLGFYHVGATRCLSEHAPHLLRDARMLFGASAGALHCVGVLSGIPLEQTLQVLS DLVRKARSRNIGIFHPSFNLSKFLRQGLGKCLPANVHQLISGKIGISLTRVSDGENVLVSDFRSKDEVVD ALVCSCFMPFYSGLIPPSFRGVRYVDGGVSDNVPFIDAKTTITVSPFYGEYDICPKVKSTNFLHVDITKL SLRLCTGNLYLLSRAFVPPDLKVLGEICLRGYLDAFRFLEEKGICNRPQPGLKSSSEGMDPEVAMPSWAN MSLDSSPESAALAVRLEGDELLDHLRLSILPWDESILDTLSPRLATALSEEMKDKGGYMSKICNLLPIRI MSYVMLPCTLPVESAIAIVQRLVTWLPDMPDDVLWLQWVTSQVFTRVLMCLLPASRSQMPVSSQQASPCT PEQDWPCWTPCSPEGCPAETKAEATPRSILRSSLNFFLGNKVPAGAEGLSTFPSFSLEKSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025225 |
| ORF Size | 1443 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025225.3 |
| RefSeq Size | 2805 bp |
| RefSeq ORF | 1446 bp |
| Locus ID | 80339 |
| UniProt ID | Q9NST1 |
| Domains | Patatin |
| Protein Pathways | Glycerolipid metabolism, Glycerophospholipid metabolism, Limonene and pinene degradation, Metabolic pathways, Phenylalanine metabolism, Tyrosine metabolism |
| MW | 52.8 kDa |
| Gene Summary | The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209577L1 | Lenti ORF clone of Human patatin-like phospholipase domain containing 3 (PNPLA3), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC209577L2 | Lenti ORF clone of Human patatin-like phospholipase domain containing 3 (PNPLA3), mGFP tagged |
CNY 6,056.00 |
|
| RC209577L3 | Lenti ORF clone of Human patatin-like phospholipase domain containing 3 (PNPLA3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209577L4 | Lenti ORF clone of Human patatin-like phospholipase domain containing 3 (PNPLA3), mGFP tagged |
CNY 6,056.00 |
|
| RG209577 | PNPLA3 (tGFP-tagged) - Human patatin-like phospholipase domain containing 3 (PNPLA3) |
CNY 5,256.00 |
|
| SC110480 | PNPLA3 (untagged)-Human patatin-like phospholipase domain containing 3 (PNPLA3) |
CNY 3,656.00 |
