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PRMT10 (PRMT9) (NM_138364) Human Tagged ORF Clone
CAT＃: RC209584
PRMT10 (Myc-DDK-tagged)-Human protein arginine methyltransferase 10 (putative) (PRMT10)
ORF Plasmid: tGFP
"NM_138364" in other vectors (7)
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CNY 6,192.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PRMT10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209584 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAACTCGCGGCCCAGGTCCCGCCGAGACGCCGGGGGTGGCGCTGGGGCAGCCGGCCGGGACGAGC TGGTGTCGCGGTCCTTGCAGAGCGCAGAGCACTGTCTGGGCGTCCAGGACTTCGGCACTGCCTATGCCCA CTACCTCCTCGTGCTCAGCCTGGCGCCGGAGCTGAAACACGACGTGAAGGAAACTTTTCAGTACACACTT TTCAGATGGGCTGAAGAGCTTGATGCTCTCAGTCGGATACAAGACTTACTTGGTTGCTATGAGCAGGCCT TGGAACTGTTTCCTGATGATGAAGTGATTTGCAATAGTATGGGGGAGCATCTCTTCAGAATGGGCTTTAG GGATGAAGCAGCTGGGTATTTTCATAAAGCAGTGAAGCTAAACCCTGATTTCAGTGATGCAAAGGAGAAT TTTTATCGTGTTGCAAACTGGTTGGTGGAACGCTGGCACTTTATCATGCTTAATGACACCAAGAGGAATA CAATTTATAATGCAGCAATCCAAAAGGCAGTTTGTTTGGGGTCCAAAAGTGTTTTGGACATTGGAGCAGG AACTGGAATACTAAGCATGTTTGCTAAAAAAGCTGGAGCACATTCCGTGTATGCCTGTGAGTTATCCAAG ACCATGTATGAACTTGCCTGTGATGTCGTGGCAGCAAACAAGATGGAAGCAGGGATCAAACTCTTACATA CGAAGTCACTTGACATAGAGATTCCAAAACATATTCCCGAAAGAGTGTCCCTAGTTGTAACAGAAACTGT CGATGCAGGTTTATTTGGAGAAGGAATTGTGGAGAGTTTGATTCATGCATGGGAGCATTTACTTTTACAG CCAAAGACCAAAGGTGAAAGTGCTAATTGTGAAAAGTATGGGAAAGTTATACCAGCAAGTGCTGTTATAT TTGGGATGGCAGTAGAATGTGCAGAGATAAGAAGACATCATAGAGTGGGTATTAAGGACATTGCTGGTAT CCATTTGCCAACAAATGTGAAATTTCAGAGTCCGGCTTATTCTTCTGTAGATACTGAAGAAACAATTGAA CCTTATACAACTGAAAAGATGAGTCGAGTTCCTGGAGGATATTTGGCTTTGACAGAGTGCTTTGAAATTA TGACAGTAGATTTCAACAACCTTCAGGAATTAAAAAGTCTTGCAACTAAAAAGCCTGATAAGATTGGTAT TCCTGTTATTAAAGAAGGCATACTAGATGCTATTATGGTTTGGTTTGTGCTCCAGCTTGATGATGAACAT AGTTTATCCACAAGTCCTAGTGAGGAAACATGTTGGGAACAGGCTGTCTACCCCGTACAGGACCTTGCAG ACTACTGGATAAAGCCTGGAGACCATGTGATGATGGAAGTATCTTGTCAAGACTGTTACTTAAGAATCCA GAGTATTAGTGTCTTGGGTTTGGAATGTGAAATGGATGTTGCAAAAAGTTTTACCCAGAATAAAGACTTG TTATCGTTAGGAAATGAGGCTGAACTTTGTAGTGCCCTCGCTAACCTTCAGACCAGTAAACCAGATGCTG TAGAGCAGACATGTATATTGGAATCTACAGAAATTGCTTTGCTTAACAACATCCCATATCATGAAGGCTT TAAAATGGCAATGAGCAAAGTTTTGTCTTCACTGACTCCAGAGAAACTGTATCAGACCATGGATACTCAC TGTCAGAATGAGATGAGCTCTGGAACTGGACAGAGTAATACTGTACAGAACATCCTTGAACCTTTCTACG TGTTAGATGTGTCCGAAGGCTTCTCTGTTCTGCCTGTTATTGCTGGCACACTTGGGCAGGTTAAACCATA CAGTTCTGTGGAGAAAGACCAGCATCGTATTGCTCTGGACCTCATATCTGAAGCCAATCACTTTCCTAAA GAAACACTTGAGTTTTGGCTGAGACATGTGGAGGATGAATCTGCTATGTTACAAAGGCCAAAATCAGACA AGTTATGGAGCATAATTATATTGGATGTCATTGAGCCATCTGGGCTCATTCAGCAGGAAATAATGGAAAA AGCTGCAATATCCAGGTGTTTACTACAATCTGGAGGCAAGATCTTTCCTCAGTATGTGCTGATGTTTGGG TTGCTTGTGGAATCACAGACACTCCTAGAGGAGAATGCTGTTCAAGGAACAGAACGTACTCTTGGATTAA ATATAGCACCTTTTATTAACCAGTTTCAGGTACCTATACGTGTATTTTTGGACCTATCCTCATTGCCCTG TATACCTTTAAGCAAGCCAGTGGAACTCTTAAGACTAGATTTAATGACTCCGTATTTGAACACCTCTAAC AGAGAAGTAAAGGTATACGTTTGTAAATCTGGAAGACTGACTGCTATTCCATTTTGGTATCATATGTACC TTGATGAAGAGATTAGGTTGGATACTTCAAGTGAAGCCTCCCACTGGAAACAAGCTGCAGTTGTTTTAGA TAATCCCATCCAGGTTGAAATGGGAGAGGAACTTGTACTCAGCATTCAGCATCACAAAAGCAATGTCAGC ATCACAGTAAAGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209584 protein sequence
Red=Cloning site Green=Tags(s) MSNSRPRSRRDAGGGAGAAGRDELVSRSLQSAEHCLGVQDFGTAYAHYLLVLSLAPELKHDVKETFQYTL FRWAEELDALSRIQDLLGCYEQALELFPDDEVICNSMGEHLFRMGFRDEAAGYFHKAVKLNPDFSDAKEN FYRVANWLVERWHFIMLNDTKRNTIYNAAIQKAVCLGSKSVLDIGAGTGILSMFAKKAGAHSVYACELSK TMYELACDVVAANKMEAGIKLLHTKSLDIEIPKHIPERVSLVVTETVDAGLFGEGIVESLIHAWEHLLLQ PKTKGESANCEKYGKVIPASAVIFGMAVECAEIRRHHRVGIKDIAGIHLPTNVKFQSPAYSSVDTEETIE PYTTEKMSRVPGGYLALTECFEIMTVDFNNLQELKSLATKKPDKIGIPVIKEGILDAIMVWFVLQLDDEH SLSTSPSEETCWEQAVYPVQDLADYWIKPGDHVMMEVSCQDCYLRIQSISVLGLECEMDVAKSFTQNKDL LSLGNEAELCSALANLQTSKPDAVEQTCILESTEIALLNNIPYHEGFKMAMSKVLSSLTPEKLYQTMDTH CQNEMSSGTGQSNTVQNILEPFYVLDVSEGFSVLPVIAGTLGQVKPYSSVEKDQHRIALDLISEANHFPK ETLEFWLRHVEDESAMLQRPKSDKLWSIIILDVIEPSGLIQQEIMEKAAISRCLLQSGGKIFPQYVLMFG LLVESQTLLEENAVQGTERTLGLNIAPFINQFQVPIRVFLDLSSLPCIPLSKPVELLRLDLMTPYLNTSN REVKVYVCKSGRLTAIPFWYHMYLDEEIRLDTSSEASHWKQAAVVLDNPIQVEMGEELVLSIQHHKSNVS ITVKQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138364 |
| ORF Size | 2535 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138364.4 |
| RefSeq Size | 2842 bp |
| RefSeq ORF | 2538 bp |
| Locus ID | 90826 |
| UniProt ID | Q6P2P2 |
| Protein Families | Druggable Genome |
| MW | 94.5 kDa |
| Gene Summary | This gene encodes a type II methyltransferase. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to the guanidino nitrogen atoms of arginine. The protein encoded by this gene methylates spliceosome associated protein 145 to regulate alternative splicing and acts as a modulator of small nuclear ribonucleoprotein maturation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209584L1 | Lenti ORF clone of Human protein arginine methyltransferase 10 (putative) (PRMT10), Myc-DDK-tagged |
CNY 8,592.00 |
|
| RC209584L2 | Lenti ORF clone of Human protein arginine methyltransferase 10 (putative) (PRMT10), mGFP tagged |
CNY 5,890.00 |
|
| RC209584L3 | Lenti ORF clone of Human protein arginine methyltransferase 10 (putative) (PRMT10), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209584L4 | Lenti ORF clone of Human protein arginine methyltransferase 10 (putative) (PRMT10), mGFP tagged |
CNY 5,890.00 |
|
| RG209584 | PRMT10 (tGFP-tagged) - Human protein arginine methyltransferase 10 (putative) (PRMT10) |
CNY 7,792.00 |
|
| SC107064 | PRMT10 (untagged)-Human protein arginine methyltransferase 10 (putative) (PRMT10) |
CNY 6,200.00 |
|
| SC324048 | PRMT10 (untagged)-Human protein arginine methyltransferase 10 (putative) (PRMT10) |
CNY 6,200.00 |
