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ODAD4 (NM_031421) Human Tagged ORF Clone
CAT＃: RC209614
TTC25 (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 25 (TTC25)
ORF Plasmid: tGFP
"NM_031421" in other vectors (4)
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CNY 1,200.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TTC25 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209614 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGACCCCGAAGGCGAGACCTTGCGAAGCACCTTTCCCTCTTATATGGCCGAAGGCGAGCGGCTCT ACCTGTGCGGGGAATTTTCTAAAGCCGCGCAGAGCTTCAGCAACGCTCTTTACCTTCAGGATGGAGACAA GAACTGCCTGGTTGCTCGCTCAAAGTGCTTCCTGAAGATGGGAGACTTGGAGAGATCCCTGAAGGATGCT GAGGCTTCGCTCCAGAGTGACCCAGCTTTCTGTAAGGGGATTTTGCAAAAGGCTGAGACACTGTACACCA TGGGAGACTTTGAGTTTGCCTTGGTATTCTATCATCGAGGCTACAAGCTGAGGCCTGATCGGGAATTCAG AGTTGGCATTCAGAAAGCCCAGGAAGCCATCAACAACTCAGTGGGAAGTCCTTCTTCCATTAAGCTGGAG AACAAAGGGGACCTCTCCTTCTTAAGCAAGCAGGCTGAGAATATAAAAGCCCAGCAGAAGCCTCAGCCCA TGAAACACCTCTTACACCCCACCAAGGGAGAGCCCAAGTGGAAGGCCTCGCTCAAGAGTGAGAAGACTGT CCGCCAGCTTCTGGGGGAGCTCTACGTGGACAAAGAGTATTTGGAGAAGCTCCTATTGGATGAAGACCTG ATCAAAGGCACCATGAAGGGTGGCCTGACTGTGGAGGACCTCATCATGACGGGCATCAACTACCTGGATA CTCACAGCAACTTCTGGAGGCAGCAGAAGCCGATCTACGCCAGGGAGCGGGACCGGAAGCTGATGCAAGA GAAATGGCTGCGGGACCACAAACGCCGTCCCTCACAGACAGCCCATTACATCCTCAAGAGCCTGGAGGAC ATTGATATGTTGCTCACAAGTGGCAGTGCTGAAGGGAGTCTTCAGAAAGCTGAGAAAGTGCTGAAGAAGG TACTGGAATGGAACAAGGAAGAGGTACCCAACAAGGATGAACTGGTTGGAAACTTGTATAGCTGCATAGG GAATGCCCAGATTGAGCTGGGGCAGATGGAGGCAGCCCTGCAGAGCCACAGAAAGGACCTGGAGATCGCC AAGGAATATGACCTTCCTGATGCAAAATCGAGAGCCCTTGACAACATTGGCAGAGTTTTTGCCAGAGTTG GGAAATTCCAGCAAGCCATTGACACGTGGGAAGAAAAGATCCCTCTGGCAAAAACCACCCTGGAGAAGAC CTGGCTGTTCCACGAGATCGGCCGCTGCTACTTGGAGCTGGACCAGGCCTGGCAGGCCCAGAATTATGGC GAGAAGTCCCAGCAGTGTGCCGAGGAGGAAGGGGACATTGAGTGGCAACTGAATGCCAGTGTTCTGGTGG CCCAGGCACAAGTGAAGCTGAGAGACTTCGAGTCAGCCGTGAACAATTTTGAGAAGGCCCTGGAGAGAGC AAAGCTTGTGCATAACAACGAGGCGCAGCAGGCCATCATCAGTGCCTTGGACGATGCCAACAAGGGTATC ATCAGAGAACTGAGGAAAACCAACTACGTGGAGAATCTCAAAGAAAAAAGCGAGGGAGAAGCTTCACTGT ATGAAGATAGAATAATAACAAGAGAGAAGGACATGAGGAGAGTGAGAGATGAGCCCGAGAAGGTGGTGAA GCAGTGGGACCATAGTGAGGATGAGAAAGAGACAGATGAGGACGATGAGGCTTTTGGGGAAGCTCTGCAG AGCCCAGCAAGCGGAAAGCAGAGTGTGGAAGCAGGAAAAGCCAGAAGCGATTTGGGAGCAGTTGCCAAGG GCCTGTCAGGAGAATTAGGCACAAGATCAGGAGAAACAGGCAGGAAGCTACTAGAAGCTGGCAGAAGAGA GTCAAGAGAAATTTATAGGAGGCCTTCGGGAGAATTAGAGCAAAGACTCTCAGGAGAATTCAGCAGACAG GAACCAGAAGAACTAAAGAAACTTTCAGAAGTGGGCAGAAGAGAGCCAGAAGAACTGGGAAAAACACAAT TTGGAGAAATAGGAGAAACGAAAAAAAACAGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209614 protein sequence
Red=Cloning site Green=Tags(s) MSDPEGETLRSTFPSYMAEGERLYLCGEFSKAAQSFSNALYLQDGDKNCLVARSKCFLKMGDLERSLKDA EASLQSDPAFCKGILQKAETLYTMGDFEFALVFYHRGYKLRPDREFRVGIQKAQEAINNSVGSPSSIKLE NKGDLSFLSKQAENIKAQQKPQPMKHLLHPTKGEPKWKASLKSEKTVRQLLGELYVDKEYLEKLLLDEDL IKGTMKGGLTVEDLIMTGINYLDTHSNFWRQQKPIYARERDRKLMQEKWLRDHKRRPSQTAHYILKSLED IDMLLTSGSAEGSLQKAEKVLKKVLEWNKEEVPNKDELVGNLYSCIGNAQIELGQMEAALQSHRKDLEIA KEYDLPDAKSRALDNIGRVFARVGKFQQAIDTWEEKIPLAKTTLEKTWLFHEIGRCYLELDQAWQAQNYG EKSQQCAEEEGDIEWQLNASVLVAQAQVKLRDFESAVNNFEKALERAKLVHNNEAQQAIISALDDANKGI IRELRKTNYVENLKEKSEGEASLYEDRIITREKDMRRVRDEPEKVVKQWDHSEDEKETDEDDEAFGEALQ SPASGKQSVEAGKARSDLGAVAKGLSGELGTRSGETGRKLLEAGRRESREIYRRPSGELEQRLSGEFSRQ EPEELKKLSEVGRREPEELGKTQFGEIGETKKNRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031421 |
| ORF Size | 1995 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 2310 bp |
| RefSeq ORF | 2019 bp |
| Locus ID | 83538 |
| UniProt ID | Q96NG3 |
| Domains | TPR |
| MW | 75.8 kDa |
| Gene Summary | This gene encodes a tetratricopeptide repeat domain-containing protein that localizes to ciliary axonmenes and plays a role in the docking of the outer dynein arm to cilia. Mutations in this gene cause severely reduced ciliary motility and the disorder CILD35 (ciliary dyskinesia,primary, 35). Primary ciliary dyskinesia is often associated with recurrent respiratory infections, immotile spermatozoa, and situs inversus; an inversion in left-right body symmetry. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209614L3 | Lenti ORF clone of Human tetratricopeptide repeat domain 25 (TTC25), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209614L4 | Lenti ORF clone of Human tetratricopeptide repeat domain 25 (TTC25), mGFP tagged |
CNY 5,890.00 |
|
| RG209614 | TTC25 (tGFP-tagged) - Human tetratricopeptide repeat domain 25 (TTC25) |
CNY 2,800.00 |
|
| SC322025 | TTC25 (untagged)-Human tetratricopeptide repeat domain 25 (TTC25) |
CNY 4,928.00 |
