NEK5 (NM_199289) Human Tagged ORF Clone

Name: NEK5 (NM_199289) Human Tagged ORF Clone
SKU: RC209623
Price: 5184.00

CAT＃: RC209623

NEK5 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 5 (NEK5)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_199289" in other vectors (6)

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CNY 5,184.00

货期*

现货

规格

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA350921

Rabbit Polyclonal Anti-NEK5 Antibody

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC209623 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAAGTACGATGTGATTAAGGCCATCGGGCAAGGTGCCTTCGGGAAAGCATACTTAGCTAAAGGGA AATCAGATAGCAAGCACTGTGTCATAAAAGAGATCAATTTTGAAAAGATGCCCATACAAGAAAAAGAAGC TTCAAAGAAAGAAGTGATTCTTCTGGAAAAGATGAAACATCCCAACATTGTAGCCTTCTTCAATTCATTT CAAGAGAATGGCAGGCTGTTTATTGTAATGGAATATTGTGATGGAGGGGATCTCATGAAAAGGATCAATA GACAACGGGGTGTGTTATTTAGTGAAGATCAGATCCTCGGTTGGTTTGTACAGATTTCTCTAGGACTAAA ACATATTCATGACAGGAAGATATTACACAGGGACATAAAAGCTCAGAACATTTTTCTTAGCAAGAACGGA ATGGTGGCAAAGCTTGGGGACTTTGGTATAGCAAGAGTCCTGAATAATTCCATGGAACTTGCTCGAACTT GTATTGGAACACCTTACTACCTGTCCCCAGAGATCTGTCAGAATAAACCCTACAACAATAAAACGGATAT TTGGTCTCTTGGCTGTGTCTTATATGAGCTCTGCACACTTAAACATCCTTTTGAGGGTAACAACTTACAG CAGCTGGTTCTGAAGATTTGTCAAGCACATTTTGCCCCAATATCTCCGGGGTTTTCTCGTGAGCTCCATT CCTTGATATCTCAGCTCTTTCAAGTATCTCCTCGAGACCGACCATCCATAAATTCCATTTTGAAAAGGCC CTTTTTAGAGAATCTTATTCCCAAATATTTGACTCCTGAGGTCATTCAGGAAGAATTCAGTCACATGCTT ATATGCAGAGCAGGAGCGCCAGCTTCTCGACATGCTGGGAAGGTGGTCCAGAAGTGTAAAATACAAAAAG TGAGATTCCAGGGAAAGTGCCCACCAAGATCAAGGATATCTGTGCCAATTAAAAGGAATGCTATATTGCA TAGAAATGAATGGAGACCACCAGCTGGAGCCCAGAAGGCCAGATCTATAAAAATGATAGAAAGACCCAAA ATTGCTGCTGTCTGTGGACATTATGATTATTATTATGCTCAACTTGATATGCTGAGGAGGAGAGCCCACA AACCAAGTTATCACCCTATTCCTCAAGAAAATACTGGAGTTGAGGATTACGGTCAGGAAACGAGGCATGG TCCATCCCCAAGTCAATGGCCTGCTGAGTACCTTCAGAGAAAATTTGAAGCTCAACAATATAAGTTGAAA GTGGAGAAGCAATTGGGTCTTCGTCCATCTTCTGCCGAGCCAAATTACAACCAGAGACAAGAGCTAAGAA GTAATGGAGAAGAGCCTAGATTCCAGGAGCTGCCATTTAGGAAAAACGAAATGAAGGAACAGGAATATTG GAAGCAGTTAGAGGAAATACGCCAACAGTACCACAATGACATGAAAGAAATTAGAAAGAAGATGGGGAGA GAACCAGAGGAGAACTCAAAAATAAGTCATAAAACCTATTTGGTGAAGAAGAGTAACCTGCCTGTCCATC AAGATGCATCTGAGGGAGAAGCACCTGTGCAGATGGAATTTCGCTCTTGTTGCCCAGGCTGGAGTGCAAT GGCACGATCTTGGCTCACCGCAACCTCCGCCTCCCAGGACATTGAAAAAGACTTGAAACAAATGAGGCTT CAGAACACAAAGGAAAGTAAAAATCCAGAACAGAAATATAAAGCTAAGAAGGGGGTAAAATTTGAAATTA ATTTAGACAAATGTATTTCTGATGAAAACATCCTCCAAGAGGAAGAGGCAATGGATATACCAAATGAAAC TTTGACCTTTGAGGATGGCATGAAGTTTAAGGAATATGAATGTGTAAAGGAGCATGGAGATTATACAGAC AAAGCATTTGAAAAACTTCACTGCCCAGAAGCAGGGTTTTCCACGCAGACTGTAGCTGCTGTGGGAAACA GGAGGCAGTGGGATGGAGGAGCGCCTCAGACTCTGCTGCAGATGATGGCAGTGGCCGACATCACCTCCAC CTGCCCCACGGGGCCTGACAGTGAGTCTGTGCTTAGCGTCAGTCGTCAGGAAGGGAAGACCAAGGACCCG TACAGCCCAGTGCTCATCCTGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC209623 protein sequence Red=Cloning site Green=Tags(s) MDKYDVIKAIGQGAFGKAYLAKGKSDSKHCVIKEINFEKMPIQEKEASKKEVILLEKMKHPNIVAFFNSF QENGRLFIVMEYCDGGDLMKRINRQRGVLFSEDQILGWFVQISLGLKHIHDRKILHRDIKAQNIFLSKNG MVAKLGDFGIARVLNNSMELARTCIGTPYYLSPEICQNKPYNNKTDIWSLGCVLYELCTLKHPFEGNNLQ QLVLKICQAHFAPISPGFSRELHSLISQLFQVSPRDRPSINSILKRPFLENLIPKYLTPEVIQEEFSHML ICRAGAPASRHAGKVVQKCKIQKVRFQGKCPPRSRISVPIKRNAILHRNEWRPPAGAQKARSIKMIERPK IAAVCGHYDYYYAQLDMLRRRAHKPSYHPIPQENTGVEDYGQETRHGPSPSQWPAEYLQRKFEAQQYKLK VEKQLGLRPSSAEPNYNQRQELRSNGEEPRFQELPFRKNEMKEQEYWKQLEEIRQQYHNDMKEIRKKMGR EPEENSKISHKTYLVKKSNLPVHQDASEGEAPVQMEFRSCCPGWSAMARSWLTATSASQDIEKDLKQMRL QNTKESKNPEQKYKAKKGVKFEINLDKCISDENILQEEEAMDIPNETLTFEDGMKFKEYECVKEHGDYTD KAFEKLHCPEAGFSTQTVAAVGNRRQWDGGAPQTLLQMMAVADITSTCPTGPDSESVLSVSRQEGKTKDP YSPVLILM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_199289
ORF Size	2124 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_199289.3
RefSeq Size	2949 bp
RefSeq ORF	2127 bp
Locus ID	341676
UniProt ID	Q6P3R8
Protein Families	Druggable Genome, Protein Kinase
MW	81.4 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC209623L1	Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 5 (NEK5), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,584.00
RC209623L2	Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 5 (NEK5), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC209623L3	Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 5 (NEK5), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC209623L4	Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 5 (NEK5), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG209623	NEK5 (tGFP-tagged) - Human NIMA (never in mitosis gene a)-related kinase 5 (NEK5)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,784.00
SC322758	NEK5 (untagged)-Human NIMA (never in mitosis gene a)-related kinase 5 (NEK5)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,192.00 CNY 7,220.00