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NPBWR2 (NM_005286) Human Tagged ORF Clone
CAT＃: RC209677
NPBWR2 (Myc-DDK-tagged)-Human neuropeptides B/W receptor 2 (NPBWR2)
ORF Plasmid: tGFP
"NM_005286" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GPR8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209677 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGCCGCTGGGCACCCAGAGCCCCTTGACAGCAGGGGCTCCTTCTCCCTCCCCACGATGGGTGCCA ACGTCTCTCAGGACAATGGCACTGGCCACAATGCCACCTTCTCCGAGCCACTGCCGTTCCTCTATGTGCT CCTGCCCGCCGTGTACTCCGGGATCTGTGCTGTGGGGCTGACTGGCAACACGGCCGTCATCCTTGTAATC CTAAGGGCGCCCAAGATGAAGACGGTGACCAACGTGTTCATCCTGAACCTGGCCGTCGCCGACGGGCTCT TCACGCTGGTACTGCCTGTCAACATCGCGGAGCACCTGCTGCAGTACTGGCCCTTCGGGGAGCTGCTCTG CAAGCTGGTGCTGGCCGTCGACCACTACAACATCTTCTCCAGCATCTACTTCCTAGCCGTGATGAGCGTG GACCGATACCTGGTGGTGCTGGCCACCGTGAGGTCCCGCCACATGCCCTGGCGCACCTACCGGGGGGCGA AGGTCGCCAGCCTGTGTGTCTGGCTGGGCGTCACGGTCCTGGTTCTGCCCTTCTTCTCTTTCGCTGGCGT CTACAGCAACGAGCTGCAGGTCCCAAGCTGTGGGCTGAGCTTCCCGTGGCCCGAGCAGGTCTGGTTCAAG GCCAGCCGTGTCTACACGTTGGTCCTGGGCTTCGTGCTGCCCGTGTGCACCATCTGTGTGCTCTACACAG ACCTCCTGCGCAGGCTGCGGGCCGTGCGGCTCCGCTCTGGAGCCAAGGCTCTAGGCAAGGCCAGGCGGAA GGTGACCGTCCTGGTCCTCGTCGTGCTGGCCGTGTGCCTCCTCTGCTGGACGCCCTTCCACCTGGCCTCT GTCGTGGCCCTGACCACGGACCTGCCCCAGACCCCACTGGTCATCAGTATGTCCTACGTCATCACCAGCC TCAGCTACGCCAACTCGTGCCTGAACCCCTTCCTCTACGCCTTTCTAGATGACAACTTCCGGAAGAACTT CCGCAGCATATTGCGGTGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209677 protein sequence
Red=Cloning site Green=Tags(s) MQAAGHPEPLDSRGSFSLPTMGANVSQDNGTGHNATFSEPLPFLYVLLPAVYSGICAVGLTGNTAVILVI LRAPKMKTVTNVFILNLAVADGLFTLVLPVNIAEHLLQYWPFGELLCKLVLAVDHYNIFSSIYFLAVMSV DRYLVVLATVRSRHMPWRTYRGAKVASLCVWLGVTVLVLPFFSFAGVYSNELQVPSCGLSFPWPEQVWFK ASRVYTLVLGFVLPVCTICVLYTDLLRRLRAVRLRSGAKALGKARRKVTVLVLVVLAVCLLCWTPFHLAS VVALTTDLPQTPLVISMSYVITSLSYANSCLNPFLYAFLDDNFRKNFRSILRC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005286 |
| ORF Size | 1001 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005286.4 |
| RefSeq Size | 1002 bp |
| RefSeq ORF | 1002 bp |
| Locus ID | 2832 |
| UniProt ID | P48146 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 36.9 kDa |
| Gene Summary | The protein encoded by this gene is an integral membrane protein and G protein-coupled receptor. The encoded protein is similar in sequence to another G protein-coupled receptor (GPR7), and it is structurally similar to opioid and somatostatin receptors. This protein binds neuropeptides B and W. This gene is intronless and is expressed primarily in the frontal cortex of the brain. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Development of a Robust Mammalian Cell-based Assay for Studying Recombinant α4 β1/3 δ GABAA Receptor Subtypes
,Petersen, CB;Søgaard, R;Madsen, KL;Klein, AB;Frølund, B;Wellendorph, P;,
Basic & Clinical Pharmacology & Toxicology
,PubMed ID 28299900
[NPBWR2]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209677L1 | Lenti ORF clone of Human neuropeptides B/W receptor 2 (NPBWR2), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC209677L2 | Lenti ORF clone of Human neuropeptides B/W receptor 2 (NPBWR2), mGFP tagged |
CNY 5,890.00 |
|
| RC209677L3 | Lenti ORF clone of Human neuropeptides B/W receptor 2 (NPBWR2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209677L4 | Lenti ORF clone of Human neuropeptides B/W receptor 2 (NPBWR2), mGFP tagged |
CNY 5,890.00 |
|
| RG209677 | NPBWR2 (tGFP-tagged) - Human neuropeptides B/W receptor 2 (NPBWR2) |
CNY 5,256.00 |
|
| SC116844 | NPBWR2 (untagged)-Human neuropeptides B/W receptor 2 (NPBWR2) |
CNY 3,656.00 |
