LTBP2 (NM_000428) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C14orf141; GLC3D; LTBP3; MSPKA; MSTP031; WMS3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC209838 representing NM_000428 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCCGCGGACCAAAGCCCGCAGCCCGGGGCGCGCCCTGCGGAACCCCTGGAGAGGCTTCCTGCCGC TCACCCTGGCTCTCTTCGTGGGCGCGGGTCATGCCCAAAGGGACCCCGTAGGGAGATACGAGCCGGCTGG TGGAGACGCGAATCGACTGCGGCGCCCTGGGGGCAGCTACCCGGCAGCGGCTGCAGCCAAGGTGTACAGT CTGTTCCGGGAGCAGGACGCGCCTGTCGCGGGCTTGCAGCCCGTGGAGCGGGCCCAGCCGGGCTGGGGGA GCCCCAGGAGGCCCACCGAGGCGGAGGCCAGGAGGCCGTCCCGCGCGCAGCAGTCGCGGCGTGTCCAGCC ACCTGCGCAGACCCGGAGAAGCACTCCCCTGGGCCAGCAGCAACCAGCACCCCGGACCCGGGCCGCGCCG GCTCTCCCACGCCTGGGGACCCCACAGCGGTCTGGGGCTGCGCCCCCAACCCCGCCGCGAGGGCGACTCA CGGGGAGGAACGTCTGCGGGGGACAGTGCTGCCCAGGATGGACAACAGCAAACAGCACCAACCACTGTAT CAAACCCGTTTGCGAGCCGCCGTGCCAGAACCGGGGCTCCTGCAGCCGCCCGCAGCTCTGTGTCTGCCGC TCTGGTTTCCGTGGAGCCCGCTGCGAGGAGGTCATTCCCGATGAGGAATTTGACCCCCAGAACTCCAGGC TGGCACCTCGACGCTGGGCCGAGCGTTCACCCAACCTGCGCAGGAGCAGTGCGGCTGGAGAGGGCACCTT GGCCAGAGCACAGCCGCCAGCACCACAGTCGCCGCCCGCACCACAGTCGCCACCAGCTGGGACCCTGAGT GGCCTCAGCCAGACCCACCCTTCCCAGCAGCACGTGGGGTTGTCCCGCACTGTCCGACTTCACCCGACTG CCACGGCCAGTAGCCAGCTCTCTTCCAACGCCCTGCCCCCGGGACCAGGCCTTGAGCAGAGAGATGGCAC CCAACAGGCGGTACCTCTGGAGCACCCCTCATCCCCCTGGGGGCTGAACCTCACGGAGAAAATCAAGAAG ATCAAGATCGTCTTCACTCCCACCATCTGCAAGCAGACCTGTGCCCGTGGACACTGTGCCAACAGCTGTG AGAGGGGCGACACCACCACCCTGTACAGCCAGGGCGGCCATGGGCACGATCCCAAGTCTGGCTTCCGCAT CTATTTCTGCCAGATCCCCTGCCTGAACGGAGGCCGCTGCATCGGCAGGGACGAATGCTGGTGCCCCGCC AACTCCACCGGGAAGTTCTGCCACCTGCCTATCCCGCAGCCGGACAGGGAGCCTCCAGGGAGGGGGTCCC GCCCCAGGGCCTTGCTGGAAGCCCCACTGAAGCAGTCCACTTTCACACTGCCGCTCTCCAACCAGCTGGC CTCCGTGAACCCCTCCCTGGTGAAGGTGCACATTCACCACCCACCCGAGGCCTCAGTGCAGATCCACCAG GTGGCCCAGGTGCGGGGCGGGGTGGAGGAGGCCCTAGTGGAGAACAGCGTGGAGACCAGACCCCCGCCCT GGCTGCCTGCCAGCCCTGGCCACAGCCTCTGGGACAGCAACAACATCCCTGCTCGGTCTGGAGAGCCCCC TCGGCCACTGCCCCCAGCAGCACCCAGGCCTCGAGGACTGCTGGGCCGGTGTTACCTGAACACTGTGAAC GGACAGTGTGCCAACCCTCTGCTGGAGCTGACTACCCAGGAGGACTGCTGTGGCAGTGTGGGAGCCTTCT GGGGGGTGACTTTGTGTGCCCCATGCCCACCCAGACCAGCCTCCCCGGTGATTGAGAATGGCCAGCTGGA GTGTCCTCAGGGGTACAAGAGACTGAACCTCACTCACTGCCAAGATATCAACGAGTGCTTGACCCTGGGC CTGTGCAAGGACGCGGAGTGTGTGAATACCAGGGGCAGCTACCTGTGCACATGCAGACCTGGCCTCATGC TGGATCCATCGCGGAGCCGCTGTGTGTCGGACAAGGCAATCTCCATGCTGCAGGGACTGTGCTACCGGTC GCTGGGGCCCGGCACCTGCACCCTGCCTTTGGCCCAGCGGATCACCAAGCAGATATGCTGCTGCAGCCGC GTGGGCAAAGCATGGGGCAGCGAGTGTGAGAAATGCCCTCTGCCTGGCACAGAGGCCTTCAGAGAGATCT GCCCTGCCGGCCACGGCTACACCTACGCGAGCTCCGACATCCGCCTGTCCATGAGGAAAGCCGAGGAGGA GGAACTGGCAAGGCCCCCAAGGGAGCAAGGGCAGAGGAGCAGCGGGGCACTGCCCGGGCCAGCAGAGAGG CAGCCCCTCCGGGTCGTCACGGACACCTGGCTTGAGGCCGGGACCATCCCTGACAAGGGTGACTCTCAGG CTGGCCAGGTCACGACCAGTGTCACTCATGCACCTGCCTGGGTCACAGGGAATGCCACAACCCCACCAAT GCCTGAACAGGGGATTGCAGAGATACAGGAAGAACAAGTGACCCCCTCCACCGATGTGCTGGTGACCCTG AGCACCCCAGGCATTGACAGATGCGCTGCTGGAGCCACCAACGTCTGTGGCCCTGGAACCTGCGTGAACC TCCCCGATGGATACAGATGTGTCTGCAGCCCTGGCTACCAGCTGCACCCCAGCCAGGCCTACTGCACAGA TGACAACGAGTGTCTGAGGGACCCCTGCAAGGGAAAAGGGCGCTGCATCAACCGCGTGGGGTCCTACTCC TGCTTCTGCTACCCTGGCTACACTCTGGCCACCTCAGGGGCGACACAGGAGTGTCAAGATATCAATGAGT GTGAGCAGCCAGGGGTGTGCAGCGGGGGGCAGTGCACCAACACCGAGGGCTCGTACCACTGCGAGTGTGA TCAGGGCTACATCATGGTCAGGAAAGGACACTGCCAAGATATCAACGAATGCCGTCACCCCGGTACCTGC CCTGATGGGAGATGCGTCAATTCCCCTGGCTCCTACACTTGTCTGGCCTGTGAGGAGGGCTACCGGGGCC AGAGTGGGAGCTGTGTAGATGTGAATGAGTGTCTGACTCCCGGGGTCTGTGCCCATGGAAAGTGCACCAA CCTAGAAGGCTCCTTCAGATGCTCTTGTGAGCAGGGCTATGAGGTCACCTCAGATGAGAAGGGCTGCCAA GATGTGGATGAGTGTGCCAGCCGGGCCTCATGCCCCACAGGCCTCTGCCTCAACACGGAGGGCTCCTTCG CCTGCTCTGCCTGTGAGAACGGGTACTGGGTGAATGAAGACGGCACTGCCTGTGAAGACCTAGATGAGTG TGCCTTCCCGGGAGTCTGCCCCTCCGGAGTCTGCACCAACACGGCTGGCTCCTTCTCCTGCAAGGACTGC GATGGGGGCTACCGGCCCAGCCCCCTGGGTGACTCCTGTGAAGATGTGGATGAATGTGAAGACCCCCAGA GCAGCTGCCTGGGAGGCGAGTGCAAGAACACTGTGGGCTCCTACCAGTGCCTCTGTCCCCAGGGCTTCCA GCTGGCCAATGGCACCGTGTGTGAGGATGTGAATGAGTGCATGGGGGAGGAGCACTGCGCACCACACGGC GAGTGCCTCAACAGCCACGGGTCTTTCTTCTGTCTGTGCGCGCCTGGCTTCGTCAGCGCAGAGGGGGGCA CCAGCTGCCAGGATGTGGACGAGTGTGCCACCACAGACCCGTGTGTGGGAGGGCACTGTGTCAACACCGA GGGCTCCTTCAACTGTCTATGTGAGACTGGCTTCCAGCCCTCCCCAGAGAGTGGAGAGTGTGTGGATATT GACGAGTGTGAGGACTATGGAGACCCGGTGTGTGGCACCTGGAAGTGTGAAAACAGCCCTGGCTCCTACC GCTGTGTTCTGGGCTGCCAGCCTGGCTTCCACATGGCCCCGAACGGAGACTGCATTGACATAGACGAGTG CGCCAACGACACCATGTGTGGCAGCCACGGCTTCTGTGACAACACTGATGGCTCCTTCCGCTGCCTCTGT GACCAGGGCTTCGAGATCTCTCCCTCAGGCTGGGACTGTGTGGATGTGAACGAGTGTGAGCTTATGCTGG CGGTATGTGGGGCCGCGCTCTGTGAGAACGTGGAGGGCTCCTTCCTGTGCCTCTGTGCCAGTGACCTGGA GGAGTACGATGCCCAGGAGGGGCACTGCCGCCCACGGGGGGCTGGAGGTCAGAGTATGTCTGAGGCCCCA ACGGGGGACCATGCCCCGGCCCCCACCCGCATGGACTGCTACTCCGGGCAGAAGGGCCATGCGCCCTGCT CCAGTGTCCTGGGCCGGAACACCACACAGGCTGAATGCTGCTGCACCCAGGGCGCTAGCTGGGGAGATGC CTGTGACCTCTGCCCGTCTGAGGACTCAGCTGAATTCAGCGAGATCTGCCCTAGTGGAAAAGGCTACATT CCTGTGGAAGGAGCCTGGACGTTTGGACAGACCATGTACACAGATGCGGATGAGTGTGTGATATTCGGGC CTGGTCTCTGCCCGAACGGCCGGTGCCTCAACACCGTGCCTGGTTATGTCTGCCTGTGCAATCCCGGCTT CCACTACGATGCTTCCCACAAGAAGTGTGAGGATCACGATGAGTGCCAGGACCTGGCCTGTGAGAATGGC GAGTGCGTCAACACGGAGGGCTCCTTCCACTGCTTCTGCAGCCCCCCGCTCACCCTGGACCTCAGCCAGC AGCGCTGCATGAACAGCACCAGCAGCACGGAGGACCTCCCTGACCACGACATCCACATGGACATCTGCTG GAAAAAAGTCACCAATGATGTGTGCAGCGAACCCCTGCGTGGGCACCGCACCACCTACACGGAATGCTGC TGCCAGGACGGCGAGGCCTGGAGCCAGCAGTGTGCTCTGTGTCCCCCGAGGAGCTCTGAGGTCTATGCTC AGCTGTGCAACGTGGCTCGCATTGAGGCAGAGCGGGAGGCCGGGGTCCACTTCCGGCCAGGCTATGAGTA TGGCCCCGGGCCCGATGACCTGCACTACAGCATCTATGGCCCAGATGGGGCCCCCTTCTACAACTACCTG GGCCCCGAGGACACCGTCCCTGATCCTGCCTTCCCCAACACAGCCGGTCACTCAGCGGACCGCACACCCA TCCTTGAGTCTCCTTTGCAGCCCTCAGAACTCCAGCCCCACTACGTGGCCAGCCATCCAGAGCCCCCAGC CGGCTTCGAAGGGCTTCAGGCGGAGGAGTGCGGCATCCTGAACGGCTGTGAGAATGGCCGCTGTGTGCGC GTGCGGGAGGGCTACACCTGTGACTGTTTTGAGGGCTTCCAGCTGGATGCGGCCCACATGGCCTGCGTAG ATGTGAATGAGTGTGATGACTTGAACGGGCCTGCTGTGCTCTGTGTCCATGGTTACTGCGAGAACACAGA GGGCTCCTACCGCTGCCACTGCTCCCCGGGATATGTGGCTGAGGCAGGGCCCCCCCACTGCACTGCCAAG GAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC209838 representing NM_000428 Red=Cloning site Green=Tags(s) MRPRTKARSPGRALRNPWRGFLPLTLALFVGAGHAQRDPVGRYEPAGGDANRLRRPGGSYPAAAAAKVYS LFREQDAPVAGLQPVERAQPGWGSPRRPTEAEARRPSRAQQSRRVQPPAQTRRSTPLGQQQPAPRTRAAP ALPRLGTPQRSGAAPPTPPRGRLTGRNVCGGQCCPGWTTANSTNHCIKPVCEPPCQNRGSCSRPQLCVCR SGFRGARCEEVIPDEEFDPQNSRLAPRRWAERSPNLRRSSAAGEGTLARAQPPAPQSPPAPQSPPAGTLS GLSQTHPSQQHVGLSRTVRLHPTATASSQLSSNALPPGPGLEQRDGTQQAVPLEHPSSPWGLNLTEKIKK IKIVFTPTICKQTCARGHCANSCERGDTTTLYSQGGHGHDPKSGFRIYFCQIPCLNGGRCIGRDECWCPA NSTGKFCHLPIPQPDREPPGRGSRPRALLEAPLKQSTFTLPLSNQLASVNPSLVKVHIHHPPEASVQIHQ VAQVRGGVEEALVENSVETRPPPWLPASPGHSLWDSNNIPARSGEPPRPLPPAAPRPRGLLGRCYLNTVN GQCANPLLELTTQEDCCGSVGAFWGVTLCAPCPPRPASPVIENGQLECPQGYKRLNLTHCQDINECLTLG LCKDAECVNTRGSYLCTCRPGLMLDPSRSRCVSDKAISMLQGLCYRSLGPGTCTLPLAQRITKQICCCSR VGKAWGSECEKCPLPGTEAFREICPAGHGYTYASSDIRLSMRKAEEEELARPPREQGQRSSGALPGPAER QPLRVVTDTWLEAGTIPDKGDSQAGQVTTSVTHAPAWVTGNATTPPMPEQGIAEIQEEQVTPSTDVLVTL STPGIDRCAAGATNVCGPGTCVNLPDGYRCVCSPGYQLHPSQAYCTDDNECLRDPCKGKGRCINRVGSYS CFCYPGYTLATSGATQECQDINECEQPGVCSGGQCTNTEGSYHCECDQGYIMVRKGHCQDINECRHPGTC PDGRCVNSPGSYTCLACEEGYRGQSGSCVDVNECLTPGVCAHGKCTNLEGSFRCSCEQGYEVTSDEKGCQ DVDECASRASCPTGLCLNTEGSFACSACENGYWVNEDGTACEDLDECAFPGVCPSGVCTNTAGSFSCKDC DGGYRPSPLGDSCEDVDECEDPQSSCLGGECKNTVGSYQCLCPQGFQLANGTVCEDVNECMGEEHCAPHG ECLNSHGSFFCLCAPGFVSAEGGTSCQDVDECATTDPCVGGHCVNTEGSFNCLCETGFQPSPESGECVDI DECEDYGDPVCGTWKCENSPGSYRCVLGCQPGFHMAPNGDCIDIDECANDTMCGSHGFCDNTDGSFRCLC DQGFEISPSGWDCVDVNECELMLAVCGAALCENVEGSFLCLCASDLEEYDAQEGHCRPRGAGGQSMSEAP TGDHAPAPTRMDCYSGQKGHAPCSSVLGRNTTQAECCCTQGASWGDACDLCPSEDSAEFSEICPSGKGYI PVEGAWTFGQTMYTDADECVIFGPGLCPNGRCLNTVPGYVCLCNPGFHYDASHKKCEDHDECQDLACENG ECVNTEGSFHCFCSPPLTLDLSQQRCMNSTSSTEDLPDHDIHMDICWKKVTNDVCSEPLRGHRTTYTECC CQDGEAWSQQCALCPPRSSEVYAQLCNVARIEAEREAGVHFRPGYEYGPGPDDLHYSIYGPDGAPFYNYL GPEDTVPDPAFPNTAGHSADRTPILESPLQPSELQPHYVASHPEPPAGFEGLQAEECGILNGCENGRCVR VREGYTCDCFEGFQLDAAHMACVDVNECDDLNGPAVLCVHGYCENTEGSYRCHCSPGYVAEAGPPHCTAK E TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_000428
ORF Size	5463 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_000428.3
RefSeq Size	8568 bp
RefSeq ORF	5466 bp
Locus ID	4053
UniProt ID	Q14767
Protein Families	Druggable Genome, Secreted Protein
MW	195 kDa
Gene Summary	The protein encoded by this gene belongs to the family of latent transforming growth factor (TGF)-beta binding proteins (LTBP), which are extracellular matrix proteins with multi-domain structure. This protein is the largest member of the LTBP family possessing unique regions and with most similarity to the fibrillins. It has thus been suggested that it may have multiple functions: as a member of the TGF-beta latent complex, as a structural component of microfibrils, and a role in cell adhesion. [provided by RefSeq, Jul 2008]

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