C3orf25 (EFCAB12) (NM_207307) Human Tagged ORF Clone

CAT#: RC209876

EFCAB12 (Myc-DDK-tagged)-Human chromosome 3 open reading frame 25 (C3orf25)

ORF Plasmid: DDK tGFP



  "NM_207307" in other vectors (4)

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CNY 4,256.00

CNY 4,560.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


C3orf25 mouse monoclonal antibody,clone OTI6A6
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms C3orf25
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209876 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGACGACTATGAAGCGTACCACAGTCTGTTCTTGTCGCTGCTCGGACTCTGCCCGTCTAAGACTC
CCATCAATGAAAATGCTCCCGTCTTTGATCCTGAACCGGTCATTGCCCACTGCTTCAAGCAGTTCCAGCA
GAAGGACTTCCGCCTGCCTCAGACCCGCCGGCGAATCATCATGGTGCCTCGCAAGGAGGATCAGACACCC
CTTAATCCTGCATCCCAACCTCAGGCTCCCCCAAAGCCCATCCCCAGCTTCAAAGTTCTGGAAGCTAGAG
ATATCCAAGAGCAGCCAGAGGACAGGAAGACCTGGCTGAGCCAGAGGTCGAAGCTGCGGCAGGAGCTAGA
GTCCTTTGGTGATGTAAAGAGGTGGCTGGAGAACAAGCCCAGCATCACGCCTTCAGAGGCCAAGGTCTTA
CACATGATCCACGAGGAGCAGAGTGCCCAGCCAAATGCCTCCCAGGCAACTACCAGGACCACCAGGAAGA
AAGCCCCCAGGCTCTCCCGGCTGTCCCGCCAGATGGTGCCCCAGCTCCAGCTGCCCGAGCCCCCTGCCCT
GTCGGTCATGTACTCCTACCTGCATAGCCGCAAGATCAAGATCCTGGAGATATTTCACAAGGTGGGCCAG
GGTGAGAACCAGAGAATCACCAGGGAGGAGTTCATCGCGGCTGTAAAGGCAGTCGGAGTCCCTCTGAAGA
ACCAAGAGGTGGAGGATATAGTGATCTACCTCAGCTCTCTTGGGAAGCACAACACCATCACCATGGATAT
CCTGGCCAATACCTACAAGCAGTGGTCTATGGCTCAGCAAAGGAGCAGCCTGGCCACTGCAAGGGAGCAT
TATATCTTGGCCAAGCACAGAGATTCCCTGAAGGGTCCGCTCAAGAAGCAGGAGGTGGATTCAGCCCCAC
AGCTTCCCAAAGTGGACCTACTGACGGTGCCTGCAGTCGACACGCAGATGGAGACGCGGCCCATGACCCT
GGAGGAGATGGAGGAAGTGGGCAAGCGGTACCGCGAGCGGCAGCGACAGCACAAGCTCACGATCCCCTCC
ATCCAGTACACGGAGCAATGTCACCTGGTGCGCTGTGGGAATCGGCACTTTGATGAGCACTGCCTCCCGT
CCACCATCCACGGGGATATGAGGGAGCTCATTGACTCGGCCCGCAGGCACAACTTTCTGGTCTACCTGCA
ATGCTGGAAGCTCTGTAAGTCCTATGGCCTCCCGCTGACAGAGGACATCCTCATGAAAGCCTTGCTGTAC
CCAGGAGACAAGATCATTTTCCAGATGGACAAAGTGTGCCCCATCCGGCAGCCGGGAGGCTACTACTCTG
ACTGGAAGGTCTTTTCTCCGAATCTGGCTCTGCTCCGGTCCCAGGGCCCTGGCAAGTCTAAGAGGACTGA
CAAGAAAACGCCAAAGAAAAGCAAGAAAATGCGCTTTAAGGAGTTTGAGGAATTTACCAGGAAGCTGAAG
GTGAAGAGGTCCAGTGGTCTGCAGCAAACACACCCCAATTCCTTCTGGCCGGGTCATCTTCTGGATAAGC
TGCAGCTCTACCTGCCCACTGTGGCCACAGACCGGAGCCTGGCGCTCTTCAGTTGTGTTCAACACCAGCC
CCATGTCTACCCAGCCACCTACCACCCTGACCACTGGTGGCCCCTTAGGAACAAGAACTACATGACCCAC
GCCCATTATGATGCCGCCAAGGTGTACTACATCAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209876 protein sequence
Red=Cloning site Green=Tags(s)

MDDDYEAYHSLFLSLLGLCPSKTPINENAPVFDPEPVIAHCFKQFQQKDFRLPQTRRRIIMVPRKEDQTP
LNPASQPQAPPKPIPSFKVLEARDIQEQPEDRKTWLSQRSKLRQELESFGDVKRWLENKPSITPSEAKVL
HMIHEEQSAQPNASQATTRTTRKKAPRLSRLSRQMVPQLQLPEPPALSVMYSYLHSRKIKILEIFHKVGQ
GENQRITREEFIAAVKAVGVPLKNQEVEDIVIYLSSLGKHNTITMDILANTYKQWSMAQQRSSLATAREH
YILAKHRDSLKGPLKKQEVDSAPQLPKVDLLTVPAVDTQMETRPMTLEEMEEVGKRYRERQRQHKLTIPS
IQYTEQCHLVRCGNRHFDEHCLPSTIHGDMRELIDSARRHNFLVYLQCWKLCKSYGLPLTEDILMKALLY
PGDKIIFQMDKVCPIRQPGGYYSDWKVFSPNLALLRSQGPGKSKRTDKKTPKKSKKMRFKEFEEFTRKLK
VKRSSGLQQTHPNSFWPGHLLDKLQLYLPTVATDRSLALFSCVQHQPHVYPATYHPDHWWPLRNKNYMTH
AHYDAAKVYYIN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_207307
ORF Size 1716 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_207307.3
RefSeq Size 2169 bp
RefSeq ORF 1719 bp
Locus ID 90288
UniProt ID Q6NXP0
MW 66.6 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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