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FBXL3 (NM_012158) Human Tagged ORF Clone
CAT＃: RC210074
FBXL3 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 3 (FBXL3)
ORF Plasmid: tGFP
"NM_012158" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FBL3; FBL3A; FBXL3A; IDDSFAS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210074 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACGAGGAGGAAGAGATAGTGACCGTAATTCATCAGAAGAAGGAACTGCAGAGAAATCCAAGAAAC TGAGGACTACAAATGAGCATTCTCAGACTTGTGATTGGGGTAATCTCCTTCAGGACATTATTCTCCAAGT ATTTAAATATTTGCCTCTTCTTGACCGGGCTCATGCTTCACAAGTTTGCCGCAACTGGAACCAGGTATTT CACATGCCTGACTTGTGGAGATGTTTTGAATTTGAACTGAATCAGCCAGCTACATCTTATTTGAAAGCTA CCCATCCAGAGCTGATCAAACAGATTATTAAAAGACATTCAAACCATCTACAATATGTCAGCTTCAAGGT GGACAGCAGCAAGGAATCAGCTGAAGCAGCTTGTGATATACTATCGCAACTTGTGAATTGCTCTTTAAAA ACACTTGGACTTATTTCAACTGCTCGACCAAGCTTTATGGATTTACCAAAGTCTCACTTTATCTCTGCAC TGACAGTTGTGTTCGTAAACTCCAAATCCCTGTCTTCGCTTAAGATAGATGATACTCCAGTAGATGATCC ATCTCTCAAAGTACTAGTGGCCAACAATAGTGATACACTCAAGCTGTTGAAAATGAGCAGCTGTCCTCAT GTCTCTCCAGCAGGTATCCTTTGTGTGGCTGATCAGTGTCACGGCTTAAGAGAACTAGCCCTGAACTACC ACTTATTGAGTGATGAGTTGTTACTTGCATTGTCTTCTGAAAAACATGTTCGATTAGAACATTTGCGCAT TGATGTAGTCAGTGAGAATCCTGGACAGACACACTTCCATACTATTCAGAAGAGTAGCTGGGATGCTTTC ATCAGACATTCACCCAAAGTGAACTTAGTGATGTATTTTTTTTTATATGAAGAAGAATTTGACCCCTTCT TTCGCTATGAAATACCTGCCACCCATCTGTACTTTGGGAGATCAGTAAGCAAAGATGTGCTTGGCCGTGT GGGAATGACATGCCCTAGACTGGTTGAACTAGTAGTGTGTGCAAATGGATTACGGCCACTTGATGAAGAG TTAATTCGCATTGCAGAACGTTGCAAAAATTTGTCAGCTATTGGACTAGGGGAATGTGAAGTCTCATGTA GTGCCTTTGTTGAGTTTGTGAAGATGTGTGGTGGCCGCCTATCTCAATTATCCATTATGGAAGAAGTACT AATTCCTGACCAAAAGTATAGTTTGGAGCAGATTCACTGGGAAGTGTCCAAGCATCTTGGTAGGGTGTGG TTTCCCGACATGATGCCCACTTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210074 protein sequence
Red=Cloning site Green=Tags(s) MKRGGRDSDRNSSEEGTAEKSKKLRTTNEHSQTCDWGNLLQDIILQVFKYLPLLDRAHASQVCRNWNQVF HMPDLWRCFEFELNQPATSYLKATHPELIKQIIKRHSNHLQYVSFKVDSSKESAEAACDILSQLVNCSLK TLGLISTARPSFMDLPKSHFISALTVVFVNSKSLSSLKIDDTPVDDPSLKVLVANNSDTLKLLKMSSCPH VSPAGILCVADQCHGLRELALNYHLLSDELLLALSSEKHVRLEHLRIDVVSENPGQTHFHTIQKSSWDAF IRHSPKVNLVMYFFLYEEEFDPFFRYEIPATHLYFGRSVSKDVLGRVGMTCPRLVELVVCANGLRPLDEE LIRIAERCKNLSAIGLGECEVSCSAFVEFVKMCGGRLSQLSIMEEVLIPDQKYSLEQIHWEVSKHLGRVW FPDMMPTW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012158 |
| ORF Size | 1284 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012158.4 |
| RefSeq Size | 3572 bp |
| RefSeq ORF | 1287 bp |
| Locus ID | 26224 |
| UniProt ID | Q9UKT7 |
| Domains | F-box |
| Protein Families | Druggable Genome |
| MW | 48.7 kDa |
| Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats and is localized in the nucleus. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210074L1 | Lenti ORF clone of Human F-box and leucine-rich repeat protein 3 (FBXL3), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210074L2 | Lenti ORF clone of Human F-box and leucine-rich repeat protein 3 (FBXL3), mGFP tagged |
CNY 5,890.00 |
|
| RC210074L3 | Lenti ORF clone of Human F-box and leucine-rich repeat protein 3 (FBXL3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210074L4 | Lenti ORF clone of Human F-box and leucine-rich repeat protein 3 (FBXL3), mGFP tagged |
CNY 5,890.00 |
|
| RG210074 | FBXL3 (tGFP-tagged) - Human F-box and leucine-rich repeat protein 3 (FBXL3) |
CNY 5,256.00 |
|
| SC111230 | FBXL3 (untagged)-Human F-box and leucine-rich repeat protein 3 (FBXL3) |
CNY 3,656.00 |
|
| SC324339 | FBXL3 (untagged)-Human F-box and leucine-rich repeat protein 3 (FBXL3) |
CNY 3,656.00 |
