RPA4 (NM_013347) Human Tagged ORF Clone
CAT#: RC210239
RPA4 (Myc-DDK-tagged)-Human replication protein A4, 30kDa (RPA4)
ORF Plasmid: tGFP
"NM_013347" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HSU24186 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210239 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAAGAGTGGGTTTGGGAGCTATGGCAGCATTTCTGCTGCTGATGGAGCGAGTGGAGGCAGTGACC AACTGTGTGAGAGAGATGCAACTCCTGCTATTAAGACCCAAAGACCTAAGGTCCGAATTCAGGACGTTGT ACCGTGTAATGTGAACCAGCTTCTCAGCTCTACTGTGTTTGACCCTGTGTTCAAGGTTAGGGGAATTATA GTTTCCCAGGTCTCCATCGTGGGGGTAATCAGAGGGGCAGAGAAGGCTTCAAATCACATTTGTTACAAAA TTGATGATATGACCGCGAAACCAATCGAGGCCCGACAGTGGTTTGGTAGAGAGAAAGTCAAGCAGGTGAC TCCATTGTCAGTCGGAGTATATGTCAAAGTGTTTGGTATCCTCAAATGTCCCACGGGAACAAAGAGCCTT GAGGTATTGAAAATTCATGTCCTAGAGGACATGAACGAGTTCACCGTGCATATTCTGGAAACGGTCAATG CACACATGATGCTGGATAAAGCCCGTCGTGATACCACTGTAGAAAGTGTGCCTGTGTCTCCATCAGAAGT GAATGATGCTGGGGATAACGATGAGAGTCACCGCAATTTCATCCAGGACGAAGTGCTGCGTTTGATTCAT GAGTGTCCTCATCAGGAAGGGAAGAGCATCCATGAGCTCCGGGCTCAGCTCTGCGACCTTAGCGTCAAGG CCATCAAGGAAGCGATTGATTATCTGACCGTTGAGGGCCACATCTATCCCACTGTGGATCGGGAGCATTT TAAGTCTGCTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210239 protein sequence
Red=Cloning site Green=Tags(s) MSKSGFGSYGSISAADGASGGSDQLCERDATPAIKTQRPKVRIQDVVPCNVNQLLSSTVFDPVFKVRGII VSQVSIVGVIRGAEKASNHICYKIDDMTAKPIEARQWFGREKVKQVTPLSVGVYVKVFGILKCPTGTKSL EVLKIHVLEDMNEFTVHILETVNAHMMLDKARRDTTVESVPVSPSEVNDAGDNDESHRNFIQDEVLRLIH ECPHQEGKSIHELRAQLCDLSVKAIKEAIDYLTVEGHIYPTVDREHFKSAD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_013347 |
ORF Size | 783 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013347.4, NP_037479.1 |
RefSeq Size | 1560 bp |
RefSeq ORF | 786 bp |
Locus ID | 29935 |
UniProt ID | Q13156 |
Protein Families | Druggable Genome |
Protein Pathways | DNA replication, Homologous recombination, Mismatch repair, Nucleotide excision repair |
MW | 28.9 kDa |
Gene Summary | This gene encodes a single-stranded DNA-binding protein that is the 30-kDa subunit of the replication protein A complex. Replication protein A is an essential factor for DNA double-strand break repair and cell cycle checkpoint activation. The encoded protein localizes to DNA repair foci and may be involved in the cellular DNA damage response. This protein may also play a role in inhibiting viral replication.[provided by RefSeq, Apr 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210239L3 | Lenti ORF clone of Human replication protein A4, 30kDa (RPA4), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC210239L4 | Lenti ORF clone of Human replication protein A4, 30kDa (RPA4), mGFP tagged |
CNY 5,890.00 |
|
RG210239 | RPA4 (tGFP-tagged) - Human replication protein A4, 30kDa (RPA4) |
CNY 4,000.00 |
|
SC303998 | RPA4 (untagged)-Human replication protein A4, 30kDa (RPA4) |
CNY 3,990.00 |