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5HT1E Receptor (HTR1E) (NM_000865) Human Tagged ORF Clone
CAT＃: RC210240
HTR1E (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 1E (HTR1E)
ORF Plasmid: tGFP
"NM_000865" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5-HT1E |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210240 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACATCACAAACTGTACCACAGAGGCCAGCATGGCTATAAGACCCAAGACCATCACTGAGAAGATGC TCATTTGCATGACTCTGGTGGTCATCACCACCCTCACCACGTTGCTGAACTTGGCTGTGATCATGGCTAT TGGCACCACCAAGAAGCTCCACCAGCCTGCCAACTACCTAATCTGTTCTCTGGCCGTGACGGACCTCCTG GTGGCAGTGCTCGTCATGCCCCTGAGCATCATCTACATTGTCATGGATCGCTGGAAGCTTGGGTACTTCC TCTGTGAGGTGTGGCTGAGTGTGGACATGACCTGCTGCACCTGCTCCATCCTCCACCTCTGTGTCATTGC CCTGGACAGGTACTGGGCCATCACCAATGCTATTGAATACGCCAGGAAGAGGACGGCCAAGAGGGCCGCG CTGATGATCCTTACCGTCTGGACCATCTCCATTTTCATCTCCATGCCCCCTCTGTTCTGGAGAAGCCACC GCCGCCTAAGCCCTCCCCCTAGTCAGTGCACCATCCAGCATGACCATGTTATCTACACCATTTACTCCAC GCTGGGTGCGTTTTATATCCCCTTGACTTTGATACTGATTCTCTATTACCGGATTTACCACGCGGCCAAG AGCCTTTACCAGAAAAGGGGATCAAGTCGGCACTTAAGCAACAGAAGCACAGATAGCCAGAATTCTTTTG CAAGTTGTAAACTTACACAGACTTTCTGTGTGTCTGACTTCTCCACCTCAGACCCTACCACAGAGTTTGA AAAGTTCCATGCCTCCATCAGGATCCCCCCCTTCGACAATGATCTAGATCACCCAGGAGAACGTCAGCAG ATCTCTAGCACCAGGGAACGGAAGGCAGCACGCATCCTGGGGCTGATTCTGGGTGCATTCATTTTATCCT GGCTGCCATTTTTCATCAAAGAGTTGATTGTGGGTCTGAGCATCTACACCGTGTCCTCGGAAGTGGCCGA CTTTCTGACGTGGCTCGGTTATGTGAATTCTCTGATCAACCCTCTGCTCTATACGAGTTTTAATGAAGAC TTTAAGCTGGCTTTTAAAAAGCTCATTAGATGCCGAGAGCATACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210240 protein sequence
Red=Cloning site Green=Tags(s) MNITNCTTEASMAIRPKTITEKMLICMTLVVITTLTTLLNLAVIMAIGTTKKLHQPANYLICSLAVTDLL VAVLVMPLSIIYIVMDRWKLGYFLCEVWLSVDMTCCTCSILHLCVIALDRYWAITNAIEYARKRTAKRAA LMILTVWTISIFISMPPLFWRSHRRLSPPPSQCTIQHDHVIYTIYSTLGAFYIPLTLILILYYRIYHAAK SLYQKRGSSRHLSNRSTDSQNSFASCKLTQTFCVSDFSTSDPTTEFEKFHASIRIPPFDNDLDHPGERQQ ISSTRERKAARILGLILGAFILSWLPFFIKELIVGLSIYTVSSEVADFLTWLGYVNSLINPLLYTSFNED FKLAFKKLIRCREHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000865 |
| ORF Size | 1095 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000865.3 |
| RefSeq Size | 2067 bp |
| RefSeq ORF | 1098 bp |
| Locus ID | 3354 |
| UniProt ID | P28566 |
| Domains | 7tm_1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 41.7 kDa |
| Gene Summary | G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210240L3 | Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 1E (HTR1E), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210240L4 | Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 1E (HTR1E), mGFP tagged |
CNY 5,890.00 |
|
| RG210240 | HTR1E (tGFP-tagged) - Human 5-hydroxytryptamine (serotonin) receptor 1E (HTR1E) |
CNY 5,256.00 |
|
| SC119614 | HTR1E (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 1E (HTR1E) |
CNY 3,656.00 |
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