GART (NM_000819) Human Tagged ORF Clone
CAT#: RC210447
- TrueORF®
GART (Myc-DDK-tagged)-Human phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (GART), transcript variant 1
ORF Plasmid: tGFP
"NM_000819" in other vectors (4)
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CNY 7,032.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AIRS; GARS; GARTF; PAIS; PGFT; PRGS |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210447 representing NM_000819
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCCGAGTACTTATAATTGGCAGTGGAGGAAGGGAACATACGCTGGCCTGGAAACTTGCACAGT CTCATCATGTCAAACAAGTGTTGGTTGCCCCAGGAAACGCAGGCACTGCCTGCTCTGAAAAGATTTCAAA TACCGCCATCTCAATCAGTGACCACACTGCCCTTGCTCAATTCTGCAAAGAGAAGAAAATTGAATTTGTA GTTGTTGGACCAGAAGCACCTCTGGCTGCTGGGATTGTTGGGAACCTGAGGTCTGCAGGAGTGCAATGCT TTGGCCCAACAGCAGAAGCGGCTCAGTTAGAGTCCAGCAAAAGGTTTGCCAAAGAGTTTATGGACAGACA TGGAATCCCAACCGCACAATGGAAGGCTTTCACCAAACCTGAAGAAGCCTGCAGCTTCATTTTGAGTGCA GACTTCCCTGCTTTGGTTGTGAAGGCCAGTGGTCTTGCAGCTGGAAAAGGGGTGATTGTTGCAAAGAGCA AAGAAGAGGCCTGCAAAGCTGTACAAGAGATCATGCAGGAGAAAGCCTTTGGGGCAGCTGGAGAAACAAT TGTCATTGAAGAACTTCTTGACGGAGAAGAGGTGTCGTGTCTGTGTTTCACTGATGGCAAGACTGTGGCC CCCATGCCCCCAGCACAGGACCATAAGCGATTACTGGAGGGAGATGGTGGCCCTAACACAGGGGGAATGG GAGCCTATTGTCCAGCCCCTCAGGTTTCTAATGATCTATTACTAAAAATTAAAGATACTGTTCTTCAGAG GACAGTGGATGGCATGCAGCAAGAGGGTACTCCATATACAGGTATTCTCTATGCTGGAATAATGCTGACC AAGAATGGCCCAAAAGTTCTAGAGTTTAATTGCCGTTTTGGTGATCCAGAGTGCCAAGTAATCCTCCCAC TTCTTAAAAGTGATCTTTATGAAGTGATTCAGTCCACCTTAGATGGACTGCTCTGCACATCTCTGCCTGT TTGGCTAGAAAACCACACCGCCCTAACTGTTGTCATGGCAAGTAAAGGTTATCCTGGAGACTACACCAAG GGTGTAGAGATAACAGGGTTTCCTGAGGCTCAAGCTCTAGGACTGGAGGTGTTCCATGCAGGCACTGCCC TCAAAAATGGCAAAGTAGTAACTCATGGGGGTAGAGTTCTTGCAGTCACAGCCATCCGGGAAAATCTCAT ATCAGCCCTTGAGGAAGCCAAGAAAGGACTAGCTGCTATAAAGTTTGAGGGAGCAATTTATAGGAAAGAC GTCGGCTTTCGTGCCATAGCTTTCCTCCAGCAGCCCAGGAGTTTGACTTACAAGGAATCTGGAGTAGATA TCGCAGCTGGAAATATGCTGGTCAAGAAAATTCAGCCTTTAGCAAAAGCCACTTCCAGATCAGGCTGTAA AGTTGATCTTGGAGGTTTTGCTGGTCTTTTTGATTTAAAAGCAGCTGGTTTCAAAGATCCCCTTCTGGCC TCTGGAACAGATGGCGTTGGAACTAAACTAAAGATTGCCCAGCTATGCAATAAACATGATACCATTGGTC AAGATTTGGTAGCAATGTGTGTTAATGATATTCTGGCACAAGGAGCAGAGCCCCTCTTCTTCCTTGATTA CTTTTCCTGTGGAAAACTTGACCTCAGTGTAACTGAAGCTGTTGTTGCTGGAATTGCTAAAGCTTGTGGA AAAGCTGGATGTGCTCTCCTTGGAGGTGAAACAGCAGAAATGCCTGACATGTATCCCCCTGGAGAGTATG ACCTAGCTGGGTTTGCCGTTGGTGCCATGGAGCGAGATCAGAAACTCCCTCACCTGGAAAGAATCACTGA GGGTGATGTTGTTGTTGGAATAGCTTCATCTGGTCTTCATAGCAATGGATTTAGCCTTGTGAGGAAAATC GTGGCAAAATCTTCCCTCCAGTACTCCTCTCCAGCACCTGATGGTTGTGGTGACCAGACTTTAGGGGACT TACTTCTCACGCCTACCAGAATCTACAGCCATTCACTGTTACCTGTCCTACGTTCAGGACATGTCAAAGC CTTTGCCCATATTACTGGTGGAGGATTACTAGAGAACATCCCCAGAGTCCTCCCTGAGAAACTTGGGGTA GATTTAGATGCCCAGACCTGGAGGATCCCCAGGGTCTTCTCATGGTTGCAGCAGGAAGGACACCTCTCTG AGGAAGAGATGGCCAGAACATTTAACTGTGGGGTTGGCGCTGTCCTTGTGGTATCAAAGGAGCAGACAGA GCAGATTCTGAGGGATATCCAGCAGCACAAGGAAGAAGCCTGGGTGATTGGCAGTGTGGTTGCACGAGCT GAAGGTTCCCCACGTGTGAAAGTCAAGAATCTGATTGAAAGCATGCAAATAAATGGGTCAGTGTTGAAGA ATGGCTCCCTGACAAATCATTTCTCTTTTGAAAAAAAAAAGGCCAGAGTGGCTGTCTTAATATCTGGAAC AGGATCGAACCTGCAAGCACTTATAGACAGTACTCGGGAACCAAATAGCTCTGCACAAATTGATATTGTT ATCTCCAACAAAGCCGCAGTAGCTGGGTTAGATAAAGCGGAAAGAGCTGGTATTCCCACTAGAGTAATTA ATCATAAACTGTATAAAAATCGTGTAGAATTTGACAGTGCAATTGACCTAGTCCTTGAAGAGTTCTCCAT AGACATAGTCTGTCTTGCAGGATTCATGAGAATTCTTTCTGGCCCCTTTGTCCAAAAGTGGAATGGAAAA ATGCTCAATATCCACCCATCCTTGCTCCCTTCTTTTAAGGGTTCAAATGCCCATGAGCAAGCCCTGGAAA CCGGAGTCACAGTTACTGGGTGCACTGTACACTTTGTAGCTGAAGATGTGGATGCTGGACAGATTATTTT GCAAGAAGCTGTTCCCGTGAAGAGGGGTGATACTGTCGCAACTCTTTCTGAAAGAGTAAAATTAGCAGAA CATAAAATATTTCCTGCAGCCCTTCAGCTGGTGGCCAGTGGAACTGTACAGCTTGGAGAAAATGGCAAGA TCTGTTGGGTTAAAGAGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210447 representing NM_000819
Red=Cloning site Green=Tags(s) MAARVLIIGSGGREHTLAWKLAQSHHVKQVLVAPGNAGTACSEKISNTAISISDHTALAQFCKEKKIEFV VVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSA DFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTVA PMPPAQDHKRLLEGDGGPNTGGMGAYCPAPQVSNDLLLKIKDTVLQRTVDGMQQEGTPYTGILYAGIMLT KNGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTLDGLLCTSLPVWLENHTALTVVMASKGYPGDYTK GVEITGFPEAQALGLEVFHAGTALKNGKVVTHGGRVLAVTAIRENLISALEEAKKGLAAIKFEGAIYRKD VGFRAIAFLQQPRSLTYKESGVDIAAGNMLVKKIQPLAKATSRSGCKVDLGGFAGLFDLKAAGFKDPLLA SGTDGVGTKLKIAQLCNKHDTIGQDLVAMCVNDILAQGAEPLFFLDYFSCGKLDLSVTEAVVAGIAKACG KAGCALLGGETAEMPDMYPPGEYDLAGFAVGAMERDQKLPHLERITEGDVVVGIASSGLHSNGFSLVRKI VAKSSLQYSSPAPDGCGDQTLGDLLLTPTRIYSHSLLPVLRSGHVKAFAHITGGGLLENIPRVLPEKLGV DLDAQTWRIPRVFSWLQQEGHLSEEEMARTFNCGVGAVLVVSKEQTEQILRDIQQHKEEAWVIGSVVARA EGSPRVKVKNLIESMQINGSVLKNGSLTNHFSFEKKKARVAVLISGTGSNLQALIDSTREPNSSAQIDIV ISNKAAVAGLDKAERAGIPTRVINHKLYKNRVEFDSAIDLVLEEFSIDIVCLAGFMRILSGPFVQKWNGK MLNIHPSLLPSFKGSNAHEQALETGVTVTGCTVHFVAEDVDAGQIILQEAVPVKRGDTVATLSERVKLAE HKIFPAALQLVASGTVQLGENGKICWVKEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000819 |
ORF Size | 3030 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000819.5 |
RefSeq Size | 3376 bp |
RefSeq ORF | 3033 bp |
Locus ID | 2618 |
UniProt ID | P22102 |
Domains | GARS, AIRS, formyl_transf |
Protein Pathways | Metabolic pathways, One carbon pool by folate, Purine metabolism |
MW | 107.8 kDa |
Gene Summary | The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210447L3 | Lenti-ORF clone of GART (Myc-DDK-tagged)-Human phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (GART), transcript variant 1 |
CNY 7,600.00 |
|
RC210447L4 | Lenti-ORF clone of GART (mGFP-tagged)-Human phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (GART), transcript variant 1 |
CNY 7,600.00 |
|
RG210447 | GART (tGFP-tagged) - Human phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (GART), transcript variant 1 |
CNY 6,270.00 |
|
SC119632 | GART (untagged)-Human phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (GART), transcript variant 1 |
CNY 7,040.00 |