CREB1 (NM_004379) Human Tagged ORF Clone

CAT＃: RC210577

  • TrueORF®

CREB1 (Myc-DDK-tagged)-Human cAMP responsive element binding protein 1 (CREB1), transcript variant A

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_004379" in other vectors (6)

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CNY 2,400.00

CNY 3,990.00

货期*
现货
规格
    • 10 ug
Cited in 7 publications.

Product images

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CREB; CREB-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210577 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCATGGAATCTGGAGCCGAGAACCAGCAGAGTGGAGATGCAGCTGTAACAGAAGCTGAAAACCAAC
AAATGACAGTTCAAGCCCAGCCACAGATTGCCACATTAGCCCAGGTATCTATGCCAGCAGCTCATGCAAC
ATCATCTGCTCCCACCGTAACTCTAGTACAGCTGCCCAATGGGCAGACAGTTCAAGTCCATGGAGTCATT
CAGGCGGCCCAGCCATCAGTTATTCAGTCTCCACAAGTCCAAACAGTTCAGATTTCAACTATTGCAGAAA
GTGAAGATTCACAGGAGTCAGTGGATAGTGTAACTGATTCCCAAAAGCGAAGGGAAATTCTTTCAAGGAG
GCCTTCCTACAGGAAAATTTTGAATGACTTATCTTCTGATGCACCAGGAGTGCCAAGGATTGAAGAAGAG
AAGTCTGAAGAGGAGACTTCAGCACCTGCCATCACCACTGTAACGGTGCCAACTCCAATTTACCAAACTA
GCAGTGGACAGTATATTGCCATTACCCAGGGAGGAGCAATACAGCTGGCTAACAATGGTACCGATGGGGT
ACAGGGCCTGCAAACATTAACCATGACCAATGCAGCAGCCACTCAGCCGGGTACTACCATTCTACAGTAT
GCACAGACCACTGATGGACAGCAGATCTTAGTGCCCAGCAACCAAGTTGTTGTTCAAGCTGCCTCTGGAG
ACGTACAAACATACCAGATTCGCACAGCACCCACTAGCACTATTGCCCCTGGAGTTGTTATGGCATCCTC
CCCAGCACTTCCTACACAGCCTGCTGAAGAAGCAGCACGAAAGAGAGAGGTCCGTCTAATGAAGAACAGG
GAAGCAGCTCGAGAGTGTCGTAGAAAGAAGAAAGAATATGTGAAATGTTTAGAAAACAGAGTGGCAGTGC
TTGAAAATCAAAACAAGACATTGATTGAGGAGCTAAAAGCACTTAAGGACCTTTACTGCCACAAATCAGA
T

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RC210577 protein sequence
Red=Cloning site Green=Tags(s)

MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVI
QAAQPSVIQSPQVQTVQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEE
KSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQY
AQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNR
EAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004379
ORF Size 981 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004379.5
RefSeq Size 9752 bp
RefSeq ORF 984 bp
Locus ID 1385
UniProt ID P16220
Domains pKID, BRLZ
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Antigen processing and presentation, Huntington's disease, Melanogenesis, Prostate cancer
MW 35.1 kDa
Gene Summary This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (7)

The use of this cDNA Clones has been cited in the following citations:
Novel live cell fluorescent probe for human-induced pluripotent stem cells highlights early reprogramming population ,Sriram, S;Kang, NY;Subramanian, S;Nandi, T;Sudhagar, S;Xing, Q;Tong, GJ;Chen, AK;Srijaya, TC;Tan, P;Loh, YH;Chang, YT;Sugii, S;, Stem cell research & therapy ,PubMed ID 33546754 [CREB1]
The endoplasmic reticulum acetyltransferases ATase1/NAT8B and ATase2/NAT8 are differentially regulated to adjust engagement of the secretory pathway ,null, Journal of neurochemistry ,PubMed ID 31945187 [CREB1]
FOXQ1 controls the induced differentiation of melanocytic cells ,Bagati, A;Bianchi-Smiraglia, A;Moparthy, S;Kolesnikova, K;Fink, EE;Kolesnikova, M;Roll, MV;Jowdy, P;Wolff, DW;Polechetti, A;Yun, DH;Lipchick, BC;Paul, LM;Wrazen, B;Moparthy, K;Mudambi, S;Morozevich, GE;Georgieva, SG;Wang, J;Shafirstein, G;Liu, S;Kandel, ES;Berman, AE;Box, NF;Paragh, G;Nikiforov, MA;, Cell Death Differ. ,PubMed ID 29463842 [CREB1]
Downregulation of microRNA-27b-3p enhances tamoxifen resistance in breast cancer by increasing NR5A2 and CREB1 expression ,Zhu, J;Zou, Z;Nie, P;Kou, X;Wu, B;Wang, S;Song, Z;He, J;, Cell Death Dis ,PubMed ID 27809310 [CREB1]
CREB Negatively Regulates IGF2R Gene Expression and Downstream Pathways to Inhibit Hypoxia-Induced H9c2 Cardiomyoblast Cell Death ,Chen, WK;Kuo, WW;Hsieh, DJ;Chang, HN;Pai, PY;Lin, KH;Pan, LF;Ho, TJ;Viswanadha, VP;Huang, CY;, Int J Mol Sci ,PubMed ID 26610485 [CREB1]
The cAMP responsive element binding protein 1 transactivates epithelial membrane protein 2, a potential tumor suppressor in the urinary bladder urothelial carcinoma ,null, Oncotarget ,PubMed ID 25940704 [CREB1]
Rit-mediated Stress Resistance Involves a p38-Mitogen- and Stress-activated Protein Kinase 1 (MSK1)-dependent cAMP Response Element-binding Protein (CREB) Activation Cascade ,Geng-Xian Shi, Weikang Cai, and Douglas A. Andres, J. Biol. Chem., Nov 2012; 287: 39859 - 39868. [CREB1]

Other Versions

SKU Description Size Price
RC210577L1 Lenti ORF clone of Human cAMP responsive element binding protein 1 (CREB1), transcript variant A, Myc-DDK-tagged
    • 10 ug

CNY 4,800.00
CNY 5,890.00

RC210577L2 Lenti ORF clone of Human cAMP responsive element binding protein 1 (CREB1), transcript variant A, mGFP tagged
    • 10 ug

CNY 4,800.00
CNY 5,890.00

RC210577L3 Lenti ORF clone of Human cAMP responsive element binding protein 1 (CREB1), transcript variant A, Myc-DDK-tagged
    • 10 ug

CNY 4,800.00
CNY 5,890.00

RC210577L4 Lenti ORF clone of Human cAMP responsive element binding protein 1 (CREB1), transcript variant A, mGFP tagged
    • 10 ug

CNY 4,800.00
CNY 5,890.00

RG210577 CREB1 (tGFP-tagged) - Human cAMP responsive element binding protein 1 (CREB1), transcript variant A
    • 10 ug

CNY 4,000.00
CNY 4,370.00

SC117388 CREB1 (untagged)-Human cAMP responsive element binding protein 1 (CREB1), transcript variant A
    • 10 ug

CNY 2,400.00
CNY 6,270.00

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