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BDH2 (NM_020139) Human Tagged ORF Clone
CAT＃: RC210586
BDH2 (Myc-DDK-tagged)-Human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2)
ORF Plasmid: tGFP
"NM_020139" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
| Cited in 3 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DHRS6; EFA6R; PRO20933; SDR15C1; UCPA-OR; UNQ6308 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210586 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCGACTTGATGGGAAAGTCATCATCCTGACGGCCGCTGCTCAGGGGATTGGCCAAGCAGCTGCCT TAGCTTTTGCAAGAGAAGGTGCCAAAGTCATAGCCACAGACATTAATGAGTCCAAACTTCAGGAACTGGA AAAGTACCCGGGTATTCAAACTCGTGTCCTTGATGTCACAAAGAAGAAACAAATTGATCAGTTTGCCAAT GAAGTTGAGAGACTTGATGTTCTCTTTAATGTTGCTGGTTTTGTCCATCATGGAACTGTCCTGGATTGTG AGGAGAAAGACTGGGACTTCTCGATGAATCTCAATGTGCGCAGCATGTACCTGATGATCAAGGCATTCCT TCCTAAAATGCTTGCTCAGAAATCTGGCAATATTATCAACATGTCTTCTGTGGCTTCCAGCGTCAAAGGA GTTGTGAACAGATGTGTGTACAGCACAACCAAGGCAGCCGTGATTGGCCTCACAAAATCTGTGGCTGCAG ATTTCATCCAGCAGGGCATCAGGTGCAACTGTGTGTGCCCAGGAACAGTTGATACGCCATCTCTACAAGA AAGAATACAAGCCAGAGGAAATCCTGAAGAGGCACGGAATGATTTCCTGAAGAGACAAAAGACGGGAAGA TTCGCAACTGCAGAAGAAATAGCCATGCTCTGCGTGTATTTGGCTTCTGATGAATCTACTTATGTAACTG GTAACCCTGTCATCATTGATGGAGGCTGGAGCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210586 protein sequence
Red=Cloning site Green=Tags(s) MGRLDGKVIILTAAAQGIGQAAALAFAREGAKVIATDINESKLQELEKYPGIQTRVLDVTKKKQIDQFAN EVERLDVLFNVAGFVHHGTVLDCEEKDWDFSMNLNVRSMYLMIKAFLPKMLAQKSGNIINMSSVASSVKG VVNRCVYSTTKAAVIGLTKSVAADFIQQGIRCNCVCPGTVDTPSLQERIQARGNPEEARNDFLKRQKTGR FATAEEIAMLCVYLASDESTYVTGNPVIIDGGWSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020139 |
| ORF Size | 735 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020139.4 |
| RefSeq Size | 2936 bp |
| RefSeq ORF | 738 bp |
| Locus ID | 56898 |
| UniProt ID | Q9BUT1 |
| Domains | adh_short |
| Protein Families | Druggable Genome |
| Protein Pathways | Butanoate metabolism, Metabolic pathways, Synthesis and degradation of ketone bodies |
| MW | 26.8 kDa |
| Gene Summary | Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron assimilation and homeostasis. Plays a role in susceptibility to bacterial infection by providing an assimilable source of iron that is exploited by pathogenic bacteria (By similarity). Also acts as a 3-hydroxybutyrate dehydrogenase (PubMed:16380372).[UniProtKB/Swiss-Prot Function] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Dysregulation of Ketone Body Metabolism Is Associated With Poor Prognosis for Clear Cell Renal Cell Carcinoma Patients
,Cui, W;Luo, W;Zhou, X;Lu, Y;Xu, W;Zhong, S;Feng, G;Liang, Y;Liang, L;Mo, Y;Xiao, X;Huang, G;Matskova, L;Zhang, Z;Li, P;Zhou, X;,
Front Oncol
,PubMed ID 31921677
[BDH2]
|
|
Inactivation of 3-hydroxybutyrate dehydrogenase type 2 promotes proliferation and metastasis of nasopharyngeal carcinoma by iron retention
,Li, B;Liao, Z;Mo, Y;Zhao, W;Zhou, X;Xiao, X;Cui, W;Feng, G;Zhong, S;Liang, Y;Du, C;Huang, G;Li, P;Xiao, X;Zhou, X;Wang, R;Zhang, Z;,
Br. J. Cancer
,PubMed ID 31819181
[BDH2]
|
|
Downregulation of BDH2 modulates iron homeostasis and promotes DNA demethylation in CD4+ T cells of systemic lupus erythematosus
,Zhao, M;Li, MY;Gao, XF;Jia, SJ;Gao, KQ;Zhou, Y;Zhang, HH;Huang, Y;Wang, J;Wu, HJ;Lu, QJ;,
Clin. Immunol.2017
,PubMed ID 29113828
[BDH2]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210586L3 | Lenti ORF clone of Human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210586L4 | Lenti ORF clone of Human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2), mGFP tagged |
CNY 5,890.00 |
|
| RG210586 | BDH2 (tGFP-tagged) - Human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2) |
CNY 4,000.00 |
|
| SC113190 | BDH2 (untagged)-Human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2) |
CNY 2,400.00 |
