MGP (NM_000900) Human Tagged ORF Clone
CAT#: RC210666
MGP (Myc-DDK-tagged)-Human matrix Gla protein (MGP), transcript variant 2
ORF Plasmid: tGFP
"NM_000900" in other vectors (6)
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CNY 1,800.00
CNY 3,705.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GIG36; MGLAP; NTI |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210666 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGCCTGATCCTTCTTGCCATCCTGGCCGCCTTAGCGGTAGTAACTTTGTGTTGTGAATCACATG AAAGCATGGAATCTTATGAACTTAATCCCTTCATTAACAGGAGAAATGCAAATACCTTCATATCCCCTCA GCAGAGATGGAGAGCTAAAGTCCAAGAGAGGATCCGAGAACGCTCTAAGCCTGTCCACGAGCTCAATAGG GAAGCCTGTGATGACTACAGACTTTGCGAACGCTACGCCATGGTTTATGGATACAATGCTGCCTATAATC GCTACTTCAGGAAGCGCCGAGGGACCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210666 protein sequence
Red=Cloning site Green=Tags(s) MKSLILLAILAALAVVTLCCESHESMESYELNPFINRRNANTFISPQQRWRAKVQERIRERSKPVHELNR EACDDYRLCERYAMVYGYNAAYNRYFRKRRGTK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000900 |
ORF Size | 309 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000900.5 |
RefSeq Size | 1398 bp |
RefSeq ORF | 312 bp |
Locus ID | 4256 |
UniProt ID | P08493 |
Domains | GLA |
Protein Families | Secreted Protein |
MW | 12.3 kDa |
Gene Summary | This gene encodes a member of the osteocalcin/matrix Gla family of proteins. The encoded vitamin K-dependent protein is secreted by chondrocytes and vascular smooth muscle cells, and functions as a physiological inhibitor of ectopic tissue calcification. Carboxylation status of the encoded protein is associated with calcification of the vasculature in human patients with cardiovascular disease and calcification of the synovial membranes in osteoarthritis patients. Mutations in this gene cause Keutel syndrome in human patients, which is characterized by abnormal cartilage calcification, peripheral pulmonary stenosis and facial hypoplasia. [provided by RefSeq, Sep 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210666L1 | Lenti ORF clone of Human matrix Gla protein (MGP), transcript variant 2, Myc-DDK-tagged |
CNY 4,200.00 |
|
RC210666L2 | Lenti ORF clone of Human matrix Gla protein (MGP), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC210666L3 | Lenti ORF clone of Human matrix Gla protein (MGP), transcript variant 2, Myc-DDK-tagged |
CNY 4,200.00 |
|
RC210666L4 | Lenti ORF clone of Human matrix Gla protein (MGP), transcript variant 2, mGFP tagged |
CNY 4,200.00 |
|
RG210666 | MGP (tGFP-tagged) - Human matrix Gla protein (MGP), transcript variant 2 |
CNY 3,400.00 |
|
SC119598 | MGP (untagged)-Human matrix Gla protein (MGP), transcript variant 2 |
CNY 1,800.00 |