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PMS2 (NM_000535) Human Tagged ORF Clone
CAT＃: RC210880
PMS2 (Myc-DDK-tagged)-Human PMS2 postmeiotic segregation increased 2 (S. cerevisiae) (PMS2), transcript variant 1
ORF Plasmid: tGFP
"NM_000535" in other vectors (6)
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CNY 9,472.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HNPCC4; MLH4; MMRCS4; PMS2CL; PMSL2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210880 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGAGCTGAGAGCTCGAGTACAGAACCTGCTAAGGCCATCAAACCTATTGATCGGAAGTCAGTCC ATCAGATTTGCTCTGGGCAGGTGGTACTGAGTCTAAGCACTGCGGTAAAGGAGTTAGTAGAAAACAGTCT GGATGCTGGTGCCACTAATATTGATCTAAAGCTTAAGGACTATGGAGTGGATCTTATTGAAGTTTCAGAC AATGGATGTGGGGTAGAAGAAGAAAACTTCGAAGGCTTAACTCTGAAACATCACACATCTAAGATTCAAG AGTTTGCCGACCTAACTCAGGTTGAAACTTTTGGCTTTCGGGGGGAAGCTCTGAGCTCACTTTGTGCACT GAGCGATGTCACCATTTCTACCTGCCACGCATCGGCGAAGGTTGGAACTCGACTGATGTTTGATCACAAT GGGAAAATTATCCAGAAAACCCCCTACCCCCGCCCCAGAGGGACCACAGTCAGCGTGCAGCAGTTATTTT CCACACTACCTGTGCGCCATAAGGAATTTCAAAGGAATATTAAGAAGGAGTATGCCAAAATGGTCCAGGT CTTACATGCATACTGTATCATTTCAGCAGGCATCCGTGTAAGTTGCACCAATCAGCTTGGACAAGGAAAA CGACAGCCTGTGGTATGCACAGGTGGAAGCCCCAGCATAAAGGAAAATATCGGCTCTGTGTTTGGGCAGA AGCAGTTGCAAAGCCTCATTCCTTTTGTTCAGCTGCCCCCTAGTGACTCCGTGTGTGAAGAGTACGGTTT GAGCTGTTCGGATGCTCTGCATAATCTTTTTTACATCTCAGGTTTCATTTCACAATGCACGCATGGAGTT GGAAGGAGTTCAACAGACAGACAGTTTTTCTTTATCAACCGGCGGCCTTGTGACCCAGCAAAGGTCTGCA GACTCGTGAATGAGGTCTACCACATGTATAATCGACACCAGTATCCATTTGTTGTTCTTAACATTTCTGT TGATTCAGAATGCGTTGATATCAATGTTACTCCAGATAAAAGGCAAATTTTGCTACAAGAGGAAAAGCTT TTGTTGGCAGTTTTAAAGACCTCTTTGATAGGAATGTTTGATAGTGATGTCAACAAGCTAAATGTCAGTC AGCAGCCACTGCTGGATGTTGAAGGTAACTTAATAAAAATGCATGCAGCGGATTTGGAAAAGCCCATGGT AGAAAAGCAGGATCAATCCCCTTCATTAAGGACTGGAGAAGAAAAAAAAGACGTGTCCATTTCCAGACTG CGAGAGGCCTTTTCTCTTCGTCACACAACAGAGAACAAGCCTCACAGCCCAAAGACTCCAGAACCAAGAA GGAGCCCTCTAGGACAGAAAAGGGGTATGCTGTCTTCTAGCACTTCAGGTGCCATCTCTGACAAAGGCGT CCTGAGACCTCAGAAAGAGGCAGTGAGTTCCAGTCACGGACCCAGTGACCCTACGGACAGAGCGGAGGTG GAGAAGGACTCGGGGCACGGCAGCACTTCCGTGGATTCTGAGGGGTTCAGCATCCCAGACACGGGCAGTC ACTGCAGCAGCGAGTATGCGGCCAGCTCCCCAGGGGACAGGGGCTCGCAGGAACATGTGGACTCTCAGGA GAAAGCGCCTGAAACTGACGACTCTTTTTCAGATGTGGACTGCCATTCAAACCAGGAAGATACCGGATGT AAATTTCGAGTTTTGCCTCAGCCAACTAATCTCGCAACCCCAAACACAAAGCGTTTTAAAAAAGAAGAAA TTCTTTCCAGTTCTGACATTTGTCAAAAGTTAGTAAATACTCAGGACATGTCAGCCTCTCAGGTTGATGT AGCTGTGAAAATTAATAAGAAAGTTGTGCCCCTGGACTTTTCTATAAGTTCTTTAGCTAAACGAATAAAG CAGTTACATCATGAAGCACAGCAAAGTGAAGGGGAACAGAATTACAGGAAGTTTAGGGCAAAGATTTGTC CTGGAGAAAATCAAGCAGCCGAAGATGAACTAAGAAAAGAGATAAGTAAAACGATGTTTGCAGAAATGGA AATCATTGGTCAGTTTAACCTGGGATTTATAATAACCAAACTGAATGAGGATATCTTCATAGTGGACCAG CATGCCACGGACGAGAAGTATAACTTCGAGATGCTGCAGCAGCACACCGTGCTCCAGGGGCAGAGGCTCA TAGCACCTCAGACTCTCAACTTAACTGCTGTTAATGAAGCTGTTCTGATAGAAAATCTGGAAATATTTAG AAAGAATGGCTTTGATTTTGTTATCGATGAAAATGCTCCAGTCACTGAAAGGGCTAAACTGATTTCCTTG CCAACTAGTAAAAACTGGACCTTCGGACCCCAGGACGTCGATGAACTGATCTTCATGCTGAGCGACAGCC CTGGGGTCATGTGCCGGCCTTCCCGAGTCAAGCAGATGTTTGCCTCCAGAGCCTGCCGGAAGTCGGTGAT GATTGGGACTGCTCTTAACACAAGCGAGATGAAGAAACTGATCACCCACATGGGGGAGATGGACCACCCC TGGAACTGTCCCCATGGAAGGCCAACCATGAGACACATCGCCAACCTGGGTGTCATTTCTCAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210880 protein sequence
Red=Cloning site Green=Tags(s) MERAESSSTEPAKAIKPIDRKSVHQICSGQVVLSLSTAVKELVENSLDAGATNIDLKLKDYGVDLIEVSD NGCGVEEENFEGLTLKHHTSKIQEFADLTQVETFGFRGEALSSLCALSDVTISTCHASAKVGTRLMFDHN GKIIQKTPYPRPRGTTVSVQQLFSTLPVRHKEFQRNIKKEYAKMVQVLHAYCIISAGIRVSCTNQLGQGK RQPVVCTGGSPSIKENIGSVFGQKQLQSLIPFVQLPPSDSVCEEYGLSCSDALHNLFYISGFISQCTHGV GRSSTDRQFFFINRRPCDPAKVCRLVNEVYHMYNRHQYPFVVLNISVDSECVDINVTPDKRQILLQEEKL LLAVLKTSLIGMFDSDVNKLNVSQQPLLDVEGNLIKMHAADLEKPMVEKQDQSPSLRTGEEKKDVSISRL REAFSLRHTTENKPHSPKTPEPRRSPLGQKRGMLSSSTSGAISDKGVLRPQKEAVSSSHGPSDPTDRAEV EKDSGHGSTSVDSEGFSIPDTGSHCSSEYAASSPGDRGSQEHVDSQEKAPETDDSFSDVDCHSNQEDTGC KFRVLPQPTNLATPNTKRFKKEEILSSSDICQKLVNTQDMSASQVDVAVKINKKVVPLDFSISSLAKRIK QLHHEAQQSEGEQNYRKFRAKICPGENQAAEDELRKEISKTMFAEMEIIGQFNLGFIITKLNEDIFIVDQ HATDEKYNFEMLQQHTVLQGQRLIAPQTLNLTAVNEAVLIENLEIFRKNGFDFVIDENAPVTERAKLISL PTSKNWTFGPQDVDELIFMLSDSPGVMCRPSRVKQMFASRACRKSVMIGTALNTSEMKKLITHMGEMDHP WNCPHGRPTMRHIANLGVISQN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000535 |
| ORF Size | 2586 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000535.7 |
| RefSeq Size | 2851 bp |
| RefSeq ORF | 2589 bp |
| Locus ID | 5395 |
| UniProt ID | P54278 |
| Domains | DNA_mis_repair, HATPase_c |
| Protein Families | Druggable Genome |
| Protein Pathways | Mismatch repair |
| MW | 95.8 kDa |
| Gene Summary | The protein encoded by this gene is a key component of the mismatch repair system that functions to correct DNA mismatches and small insertions and deletions that can occur during DNA replication and homologous recombination. This protein forms heterodimers with the gene product of the mutL homolog 1 (MLH1) gene to form the MutL-alpha heterodimer. The MutL-alpha heterodimer possesses an endonucleolytic activity that is activated following recognition of mismatches and insertion/deletion loops by the MutS-alpha and MutS-beta heterodimers, and is necessary for removal of the mismatched DNA. There is a DQHA(X)2E(X)4E motif found at the C-terminus of the protein encoded by this gene that forms part of the active site of the nuclease. Mutations in this gene have been associated with hereditary nonpolyposis colorectal cancer (HNPCC; also known as Lynch syndrome) and Turcot syndrome. [provided by RefSeq, Apr 2016] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Cancer Risk C (CR-C), a functional genomics test is a sensitive and rapid test for germline mismatch repair deficiency
,null,
Genetics in medicine : official journal of the American College of Medical Genetics
,PubMed ID 35616648
[PMS2]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210880L1 | Lenti ORF clone of Human PMS2 postmeiotic segregation increased 2 (S. cerevisiae) (PMS2), transcript variant 1, Myc-DDK-tagged |
CNY 11,872.00 |
|
| RC210880L2 | Lenti ORF clone of Human PMS2 postmeiotic segregation increased 2 (S. cerevisiae) (PMS2), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
| RC210880L3 | Lenti ORF clone of Human PMS2 postmeiotic segregation increased 2 (S. cerevisiae) (PMS2), transcript variant 1, Myc-DDK-tagged |
CNY 11,872.00 |
|
| RC210880L4 | Lenti ORF clone of Human PMS2 postmeiotic segregation increased 2 (S. cerevisiae) (PMS2), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
| RG210880 | PMS2 (tGFP-tagged) - Human PMS2 postmeiotic segregation increased 2 (S. cerevisiae) (PMS2), transcript variant 1 |
CNY 7,890.00 |
|
| SC119825 | PMS2 (untagged)-Human PMS2 postmeiotic segregation increased 2 (S. cerevisiae) (PMS2), transcript variant 1 |
CNY 6,320.00 |
