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GIRK2 (KCNJ6) (NM_002240) Human Tagged ORF Clone
CAT＃: RC210938
KCNJ6 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 6 (KCNJ6)
ORF Plasmid: tGFP
"NM_002240" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BIR1; GIRK-2; GIRK2; hiGIRK2; KATP-2; KATP2; KCNJ7; KIR3.2; KPLBS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210938 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAGCTGACAGAATCCATGACTAACGTCCTGGAGGGCGACTCCATGGATCAGGACGTCGAAAGCC CAGTGGCCATTCACCAGCCAAAGTTGCCTAAGCAGGCCAGGGATGACCTGCCAAGACACATCAGCCGAGA TCGGACCAAAAGGAAAATCCAGAGGTACGTGAGGAAAGACGGAAAGTGCAATGTTCATCACGGCAACGTG AGGGAGACCTATCGCTACCTGACCGATATCTTCACCACATTAGTGGACCTGAAGTGGAGATTCAACCTAT TGATTTTTGTCATGGTTTACACAGTGACCTGGCTCTTTTTTGGAATGATCTGGTGGTTGATCGCATACAT ACGGGGAGACATGGACCACATAGAGGACCCCTCCTGGACTCCTTGTGTTACCAACCTCAACGGGTTCGTC TCTGCTTTTTTATTCTCAATAGAGACAGAAACCACCATTGGTTATGGCTACCGGGTCATCACAGATAAAT GCCCGGAGGGAATTATTCTTCTCTTAATCCAATCTGTGTTGGGGTCCATTGTCAATGCATTCATGGTGGG ATGCATGTTTGTAAAAATCTCTCAACCCAAGAAGAGGGCAGAGACCCTGGTCTTTTCCACCCATGCAGTG ATCTCCATGCGGGATGGGAAACTGTGCCTGATGTTCCGGGTAGGGGACCTTAGGAATTCCCACATTGTGG AGGCTTCCATCAGAGCCAAGTTGATCAAATCCAAACAGACCTCGGAGGGGGAGTTCATCCCGTTGAACCA GACGGATATCAACGTAGGGTATTACACGGGGGATGACCGTCTGTTTCTGGTGTCACCGCTGATCATTAGC CATGAAATTAACCAACAGAGTCCTTTCTGGGAGATCTCCAAAGCCCAGCTGCCCAAAGAGGAACTGGAAA TTGTGGTCATCCTAGAAGGAATGGTGGAAGCCACAGGGATGACATGCCAAGCTCGAAGCTCCTACATCAC CAGTGAGATCCTGTGGGGTTACCGGTTCACACCTGTCCTGACCCTGGAGGACGGGTTCTACGAAGTTGAC TACAACAGCTTCCATGAGACCTATGAGACCAGCACCCCATCCCTTAGTGCCAAAGAGCTGGCCGAGTTAG CCAGCAGGGCAGAGCTGCCCCTGAGTTGGTCTGTATCCAGCAAACTCAACCAACATGCAGAACTGGAGAC TGAAGAGGAAGAAAAGAACCTCGAAGAGCAAACAGAAAGAAATGGTGATGTGGCAAACCTGGAGAATGAA TCCAAAGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210938 protein sequence
Red=Cloning site Green=Tags(s) MAKLTESMTNVLEGDSMDQDVESPVAIHQPKLPKQARDDLPRHISRDRTKRKIQRYVRKDGKCNVHHGNV RETYRYLTDIFTTLVDLKWRFNLLIFVMVYTVTWLFFGMIWWLIAYIRGDMDHIEDPSWTPCVTNLNGFV SAFLFSIETETTIGYGYRVITDKCPEGIILLLIQSVLGSIVNAFMVGCMFVKISQPKKRAETLVFSTHAV ISMRDGKLCLMFRVGDLRNSHIVEASIRAKLIKSKQTSEGEFIPLNQTDINVGYYTGDDRLFLVSPLIIS HEINQQSPFWEISKAQLPKEELEIVVILEGMVEATGMTCQARSSYITSEILWGYRFTPVLTLEDGFYEVD YNSFHETYETSTPSLSAKELAELASRAELPLSWSVSSKLNQHAELETEEEEKNLEEQTERNGDVANLENE SKV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002240 |
| ORF Size | 1269 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002240.5 |
| RefSeq Size | 2537 bp |
| RefSeq ORF | 1272 bp |
| Locus ID | 3763 |
| UniProt ID | P48051 |
| Domains | IRK |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 48.5 kDa |
| Gene Summary | This gene encodes a member of the G protein-coupled inwardly-rectifying potassium channel family of inward rectifier potassium channels. This type of potassium channel allows a greater flow of potassium into the cell than out of it. These proteins modulate many physiological processes, including heart rate in cardiac cells and circuit activity in neuronal cells, through G-protein coupled receptor stimulation. Mutations in this gene are associated with Keppen-Lubinsky Syndrome, a rare condition characterized by severe developmental delay, facial dysmorphism, and intellectual disability. [provided by RefSeq, Apr 2015] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Functional characterization of 5-HT1A and 5-HT1B serotonin receptor signaling through G-protein-activated inwardly rectifying K+ channels in a fluorescence-based membrane potential assay
,Gadgaard, C;Jensen, AA;,
Biochem. Pharmacol.
,PubMed ID 32088264
[GIRK2]
|
|
Discovery and Characterization of VU0529331, a Synthetic Small-Molecule Activator of Homomeric G Protein-gated, Inwardly-rectifying, Potassium (GIRK) Channels
,Kozek, KA;Du, Y;Sharma, S;Prael, FJ;Spitznagel, BD;Kharade, SV;Denton, JS;Hopkins, CR;Weaver, CD;,
ACS Chem Neurosci
,PubMed ID 30136838
[GIRK2]
|
Documents
| Product Manuals |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210938L1 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 6 (KCNJ6), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210938L2 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 6 (KCNJ6), mGFP tagged |
CNY 5,890.00 |
|
| RC210938L3 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 6 (KCNJ6), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210938L4 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 6 (KCNJ6), mGFP tagged |
CNY 5,890.00 |
|
| RG210938 | KCNJ6 (tGFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 6 (KCNJ6) |
CNY 5,256.00 |
|
| SC118770 | KCNJ6 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 6 (KCNJ6) |
CNY 3,656.00 |
