ARA9 (AIP) (NM_003977) Human Tagged ORF Clone

CAT#: RC211157

AIP (Myc-DDK-tagged)-Human aryl hydrocarbon receptor interacting protein (AIP)

ORF Plasmid: DDK tGFP



  "NM_003977" in other vectors (4)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


AIP (ARA9) mouse monoclonal antibody, clone OTI1B6 (formerly 1B6)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ARA9; FKBP16; FKBP37; PITA1; SMTPHN; XAP-2; XAP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211157 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGATATCATCGCAAGACTCCGGGAGGACGGGATCCAAAAACGTGTGATACAGGAAGGCCGAGGAG
AGCTCCCGGACTTTCAAGATGGGACCAAGGCCACGTTCCACTACCGGACGCTGCACAGTGACGACGAGGG
CACCGTGCTGGACGACAGCCGGGCTCGTGGCAAGCCCATGGAGCTCATCATTGGCAAGAAGTTCAAGCTG
CCTGTGTGGGAGACCATCGTGTGCACCATGCGAGAAGGGGAGATTGCCCAGTTCCTCTGTGACATCAAGC
ATGTGGTCCTGTACCCGCTGGTGGCCAAGAGTCTCCGCAACATCGCGGTGGGCAAGGACCCCCTGGAGGG
CCAGCGGCACTGCTGCGGTGTTGCACAGATGCGTGAACACAGCTCCCTGGGCCATGCTGACCTGGACGCC
CTGCAGCAGAACCCCCAGCCCCTCATCTTCCACATGGAGATGCTGAAGGTGGAGAGCCCTGGCACGTACC
AGCAGGACCCATGGGCCATGACAGACGAAGAGAAGGCAAAGGCAGTGCCACTTATCCACCAGGAGGGCAA
CCGGTTGTACCGCGAGGGGCATGTGAAGGAGGCTGCTGCCAAGTACTACGATGCCATTGCCTGCCTCAAG
AACCTGCAGATGAAGGAACAGCCTGGGTCCCCTGAATGGATCCAGCTGGACAAGCAGATCACGCCGCTGC
TGCTCAACTACTGCCAGTGCAAGCTGGTGGTCGAGGAGTACTACGAGGTGCTGGACCACTGCTCTTCCAT
CCTCAACAAGTACGACGACAACGTCAAGGCCTACTTCAAGCGGGGCAAGGCCCACGCGGCCGTGTGGAAT
GCCCAGGAGGCCCAGGCTGACTTTGCCAAAGTGCTGGAGCTGGACCCAGCCCTGGCGCCTGTGGTGAGCC
GAGAGCTGCGGGCCCTGGAGGCACGGATCCGGCAGAAGGACGAAGAGGACAAAGCCCGGTTCCGGGGGAT
CTTCTCCCAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211157 protein sequence
Red=Cloning site Green=Tags(s)

MADIIARLREDGIQKRVIQEGRGELPDFQDGTKATFHYRTLHSDDEGTVLDDSRARGKPMELIIGKKFKL
PVWETIVCTMREGEIAQFLCDIKHVVLYPLVAKSLRNIAVGKDPLEGQRHCCGVAQMREHSSLGHADLDA
LQQNPQPLIFHMEMLKVESPGTYQQDPWAMTDEEKAKAVPLIHQEGNRLYREGHVKEAAAKYYDAIACLK
NLQMKEQPGSPEWIQLDKQITPLLLNYCQCKLVVEEYYEVLDHCSSILNKYDDNVKAYFKRGKAHAAVWN
AQEAQADFAKVLELDPALAPVVSRELRALEARIRQKDEEDKARFRGIFSH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003977
ORF Size 990 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003977.1, NP_003968.1
RefSeq Size 1250 bp
RefSeq ORF 993 bp
Locus ID 9049
UniProt ID O00170
Protein Families Druggable Genome, Transcription Factors
MW 37.7 kDa
Gene Summary The protein encoded by this gene is a receptor for aryl hydrocarbons and a ligand-activated transcription factor. The encoded protein is found in the cytoplasm as part of a multiprotein complex, but upon binding of ligand is transported to the nucleus. This protein can regulate the expression of many xenobiotic metabolizing enzymes. Also, the encoded protein can bind specifically to and inhibit the activity of hepatitis B virus. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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