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AAK1 (NM_014911) Human Tagged ORF Clone
CAT＃: RC211284
- TrueORF®
AAK1 (Myc-DDK-tagged)-Human AP2 associated kinase 1 (AAK1)
ORF Plasmid: tGFP
"NM_014911" in other vectors (6)
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CNY 10,552.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211284 representing NM_014911.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGAAGTTTTTCGACTCCCGGCGAGAGCAGGGCGGCTCTGGCCTGGGCTCCGGCTCCAGCGGAGGA GGGGGCAGCACCTCGGGCCTGGGCAGTGGCTACATCGGAAGAGTCTTCGGCATCGGGCGACAGCAGGTC ACAGTGGACGAGGTGTTGGCGGAAGGTGGATTTGCTATTGTATTTCTGGTGAGGACAAGCAATGGGATG AAATGTGCCTTGAAACGCATGTTTGTCAACAATGAGCATGATCTCCAGGTGTGCAAGAGAGAAATCCAG ATAATGAGGGATCTTTCAGGGCACAAGAATATTGTGGGTTACATTGATTCTAGTATCAACAACGTGAGT AGCGGTGATGTATGGGAAGTGCTCATTCTGATGGACTTTTGTAGAGGTGGCCAGGTGGTAAACCTGATG AACCAGCGCCTGCAAACAGGCTTTACAGAGAATGAAGTGCTCCAGATATTTTGTGATACCTGTGAAGCT GTTGCCCGCCTGCATCAGTGCAAAACTCCTATTATCCACCGGGACCTGAAGGTTGAAAACATCCTCTTG CATGACCGAGGCCACTATGTCCTGTGTGACTTTGGAAGCGCCACCAACAAATTCCAGAATCCACAAACT GAGGGAGTCAATGCAGTAGAAGATGAGATTAAGAAATACACAACGCTGTCCTATCGAGCACCAGAAATG GTCAACCTGTACAGTGGCAAAATCATCACTACGAAGGCAGACATTTGGGCTCTTGGATGTTTGTTGTAT AAATTATGCTACTTCACTTTGCCATTTGGGGAAAGTCAGGTGGCAATTTGTGATGGAAACTTCACAATT CCTGATAATTCTCGATATTCTCAAGACATGCACTGCCTAATTAGGTATATGTTGGAACCAGACCCTGAC AAAAGGCCGGATATTTACCAGGTGTCCTACTTCTCATTTAAGCTACTCAAGAAAGAGTGCCCAATTCCA AATGTACAGAACTCTCCCATTCCTGCAAAGCTTCCTGAACCAGTGAAAGCCAGTGAGGCAGCTGCAAAA AAGACCCAGCCAAAGGCCAGACTGACAGATCCCATTCCCACCACAGAGACTTCAATTGCACCCCGCCAG AGGCCTAAAGCTGGGCAGACTCAGCCGAACCCAGGAATCCTTCCCATCCAGCCAGCGCTGACACCCCGG AAGAGGGCCACTGTTCAGCCCCCACCTCAGGCTGCAGGATCCAGCAATCAGCCTGGCCTTTTAGCCAGT GTTCCCCAACCAAAACCCCAAGCCCCACCCAGCCAGCCTCTGCCGCAAACTCAGGCCAAGCAGCCACAG GCTCCTCCCACTCCACAGCAGACGCCTTCTACTCAGGCCCAGGGTCTGCCCGCTCAGGCCCAGGCCACA CCCCAGCACCAGCAGCAACTCTTCCTCAAGCAGCAACAGCAGCAGCAACAGCCACCGCCAGCACAGCAG CAGCCGGCAGGCACGTTTTACCAGCAGCAGCAGGCCCAGACTCAGCAGTTTCAGGCAGTACATCCAGCA ACCCAGCAACCAGCAATTGCTCAGTTCCCTGTGGTGTCCCAAGGAGGCTCTCAACAGCAGCTAATGCAG AATTTCTACCAGCAGCAGCAGCAGCAGCAACAACAGCAACAGCAACAGCTGGCCACAGCCCTGCATCAA CAACAGCTGATGACTCAGCAGGCTGCCTTGCAGCAAAAGCCCACTATGGCAGCAGGACAGCAGCCCCAG CCACAGCCAGCTGCAGCCCCACAGCCAGCCCCTGCCCAGGAACCAGCGATTCAAGCCCCAGTAAGACAA CAGCCAAAGGTTCAGACAACCCCACCTCCTGCCGTCCAGGGGCAGAAAGTTGGATCTCTCACTCCACCC TCATCCCCCAAAACCCAACGTGCTGGGCACAGGCGTATTCTCAGTGACGTAACCCACAGTGCAGTCTTT GGGGTCCCTGCCAGCAAATCAACCCAGCTGCTCCAGGCAGCTGCAGCTGAGGCCAGTCTCAATAAGTCC AAGTCTGCAACCACCACTCCATCAGGCTCTCCTCGGACCTCTCAACAAAACGTTTATAATCCTTCAGAA GGGTCTACGTGGAATCCCTTTGATGACGATAATTTCTCCAAACTCACAGCTGAAGAACTGCTAAACAAG GACTTTGCCAAGCTTGGGGAAGGCAAACATCCCGAGAAGCTTGGAGGCTCAGCTGAGAGTTTGATCCCA GGCTTTCAATCAACCCAAGGTGATGCTTTTGCTACGACCTCATTTTCTGCTGGAACTGCTGAAAAAAGG AAGGGTGGGCAGACTGTGGACTCTGGCCTCCCGCTTCTAAGCGTGTCTGATCCTTTCATTCCTCTTCAA GTACCTGATGCACCAGAAAAACTAATTGAGGGACTCAAATCTCCTGACACTTCTCTTCTGCTCCCTGAC CTCTTGCCTATGACAGATCCTTTTGGTAGCACTTCTGATGCTGTAATTGAAAAAGCTGATGTTGCTGTT GAGAGTCTCATACCAGGACTGGAGCCCCCAGTTCCCCAGCGCCTCCCATCTCAGACGGAATCTGTGACC TCGAATCGCACAGATTCTCTCACCGGGGAAGATTCCCTGCTTGATTGCTCTCTGCTCTCTAACCCTACT ACTGACCTTCTGGAAGAGTTTGCCCCCACAGCAATCTCTGCTCCAGTCCATAAAGCTGCAGAAGATAGT AATCTCATCTCAGGTTTTGATGTCCCTGAGGGCTCGGACAAGGTGGCTGAAGATGAGTTTGACCCTATT CCTGTATTGATAACCAAAAACCCACAAGGTGGGCACTCTAGAAACAGCAGTGGGAGCTCTGAGTCCAGT CTTCCCAACCTAGCCAGGTCTTTACTGCTGGTGGATCAGCTCATAGACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC211284
Blue=ORF Red=Cloning site Green=Tag(s) MKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGM KCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLM NQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQT EGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTI PDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPAKLPEPVKASEAAAK KTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLAS VPQPKPQAPPSQPLPQTQAKQPQAPPTPQQTPSTQAQGLPAQAQATPQHQQQLFLKQQQQQQQPPPAQQ QPAGTFYQQQQAQTQQFQAVHPATQQPAIAQFPVVSQGGSQQQLMQNFYQQQQQQQQQQQQQLATALHQ QQLMTQQAALQQKPTMAAGQQPQPQPAAAPQPAPAQEPAIQAPVRQQPKVQTTPPPAVQGQKVGSLTPP SSPKTQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRTSQQNVYNPSE GSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKHPEKLGGSAESLIPGFQSTQGDAFATTSFSAGTAEKR KGGQTVDSGLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAV ESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLLDCSLLSNPTTDLLEEFAPTAISAPVHKAAEDS NLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014911 |
| ORF Size | 2880 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 21283 bp |
| RefSeq ORF | 2886 bp |
| Locus ID | 22848 |
| UniProt ID | Q2M2I8 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 103.8 kDa |
| Gene Summary | This gene encodes a member of the SNF1 subfamily of serine/threonine protein kinases. Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. The encoded protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. This kinase has been shown to play an important role in regulating the clathrin-mediated endocytosis of the rabies virus, facilitating infection. Inhibitors of this kinase are being studied as candidate therapeutics to disrupt the entry of viruses, including SARS-CoV-2, into target cells. It is also involved in positive regulation of Notch pathway signaling in mammals. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Aug 2020] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211284L1 | Lenti-ORF clone of AAK1 (Myc-DDK-tagged)-Human AP2 associated kinase 1 (AAK1) |
CNY 12,952.00 |
|
| RC211284L2 | Lenti-ORF clone of AAK1 (mGFP-tagged)-Human AP2 associated kinase 1 (AAK1) |
CNY 9,030.00 |
|
| RC211284L3 | Lenti-ORF clone of AAK1 (Myc-DDK-tagged)-Human AP2 associated kinase 1 (AAK1) |
CNY 9,030.00 |
|
| RC211284L4 | Lenti-ORF clone of AAK1 (mGFP-tagged)-Human AP2 associated kinase 1 (AAK1) |
CNY 9,030.00 |
|
| RG211284 | AAK1 (tGFP-tagged) - Human AP2 associated kinase 1 (AAK1) |
CNY 7,890.00 |
|
| SC316852 | AAK1 (untagged)-Human AP2 associated kinase 1 (AAK1) |
CNY 10,568.00 |
